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pLenti-CRISPRv2-sgENPP1_#1-hygro

Plasmid

#254678

Purpose

knockout human ENPP1

Depositor

Brian Ruffell

Insert

sgRNA with Cas9 and hygromycin resistance (ENPP1 Human)

Use

CRISPR and Lentiviral

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pX459-Arf1 gRNA

Plasmid

#246572

Purpose

CRISPR vector co-expressing Cas9 and a mouse Arf1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Arf1 gRNA (Arf1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-9 (nonfunctional)

Plasmid

#246292

Purpose

Evaluation of AtU6.29c13 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

AtU6.29c13 promoter (nonfunctional)

Use

CRISPR

Expression

Plant

Mutation

deletions: -A (-58 from TSS), -T (-57) within USE

Promoter

AtU6.29c13 promoter (nonfunctional)

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo24

Plasmid

#203994

Purpose

Cloning plasmid containing the Hro ES5 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Hro ES5

Expression

Plant

Promoter

Hro ES5

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-CsHPAD-AE

Plasmid

#226982

Purpose

Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase activating enzyme (HPAD-AE) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

CsHPAD-AE

Tags

His6 (on backbone)

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo25

Plasmid

#203995

Purpose

Cloning plasmid containing the Hro ES6 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Hro ES6

Expression

Plant

Promoter

Hro ES6

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo23

Plasmid

#203993

Purpose

Cloning plasmid containing the Hro ES4 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Hro ES4

Expression

Plant

Promoter

Hro ES4

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo14

Plasmid

#203984

Purpose

Cloning plasmid containing the Elu ES1 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Elu ES1

Expression

Plant

Promoter

Elu ES1

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo16

Plasmid

#203986

Purpose

Cloning plasmid containing the Elu ES3 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Elu ES3

Expression

Plant

Promoter

Elu ES3

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo18

Plasmid

#203988

Purpose

Cloning plasmid containing the Ppu ES2 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Ppu ES2

Expression

Plant

Promoter

Ppu ES2

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo19

Plasmid

#203989

Purpose

Cloning plasmid containing the Ppu ES3 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Ppu ES3

Expression

Plant

Promoter

Ppu ES3

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo22

Plasmid

#203992

Purpose

Cloning plasmid containing the Hro ES3 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Hro ES3

Expression

Plant

Promoter

Hro ES3

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 pCAGGS

Plasmid

#206101

Purpose

expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-RedO-shHsp90ab1 (#a)

Plasmid

#206357

Purpose

AAV plasmid expressing HSP90AB1 shRNA in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

shRNA #a of Hsp90ab1

Use

AAV

Promoter

human red opsin

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pET3a-FruK

Plasmid

#186256

Purpose

Overexpression of FruK for purification

Depositor

Liskin Swint-Kruse, Emile Van Schaftingen

Article

Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

Insert

1-phosphofructokinase (fruK E. coli)

Expression

Bacterial

Promoter

T7

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0061

Plasmid

#177026

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

iridoid oxidase; CYP76A26

Use

Synthetic Biology

Mutation

amplified from cDNA, contains T359S relative to K…

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pJPS2954

Plasmid

#135588

Purpose

SNR6 has UAUUUCat position 102–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6Δ6 hasUAUUUCat position 102–107 deleted

Promoter

Native

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCas9n-Nanog-R

Plasmid

#122303

Purpose

Expresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Nanog (Nanog Synthetic, Mouse)

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago1_4

Plasmid

#73536

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pYSD087

Plasmid

#252977

Purpose

Encodes Myc-tagged S. cerevisiae Sed1p display anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

Myc-tagged Sed1

Expression

Bacterial

Available Since

July 1, 2026

Availability

Academic Institutions and Nonprofits only

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