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Showing: 11961 - 11980 of 14795 results

  4. SpCas9-HF1-plus

    Plasmid
    #126768
    Purpose
    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126768-plasmid-map-sequence-id-410966

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  8. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid
    #71828
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    BACH2-sgRNA8 (BACH2 Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71828-plasmid-map-sequence-id-131023

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  9. MSP2283

    Plasmid
    #70702
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70702-plasmid-map-sequence-id-127769

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  10. pLenti EF1a VP64-NtcA-2xNLS_SV40

    Plasmid
    #254308
    Purpose
    Constitutive expression of VP64-NtcA-2xNLS_SV40 for use in the functional aKG-ON biosensor. Requires pLenti Neo glnA PCC 6803 reporter gene plasmid.
    Depositor
    Samuel McBrayer
    Article
    Sternisha et al Science. 2026 Jul 16;393(6808):eadx8675.
    Insert
    VP64-NtcA-2xNLS_SV40
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    NtcA R90E
    Promoter
    EF1a
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    254308-plasmid-map-sequence-id-508527

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  11. pLenti EF1a VP64-NtcA_R90E-2xNLS_SV40

    Plasmid
    #254309
    Purpose
    Constitutive expression of VP64- NtcA_R90E-2xNLS_SV40 for use in the inactive aKG-ON biosensor. Requires pLenti Neo glnA PCC 6803 reporter gene plasmid.
    Depositor
    Samuel McBrayer
    Article
    Sternisha et al Science. 2026 Jul 16;393(6808):eadx8675.
    Insert
    VP64-NtcA_R90E-2xNLS_SV40
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    NtcA R90E
    Promoter
    EF1a
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    254309-plasmid-map-sequence-id-508533

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  12. pLenti EF1a mCherry-T2A-VP64-NtcA-2xNLS_SV40

    Plasmid
    #254310
    Purpose
    Constitutive expression of VP64-NtcA-2xNLS_SV40 for use in the functional aKG-ON biosensor. Requires pLenti Neo glnA PCC 6803 reporter gene plasmid. mCherry to monitor NtcA chimera expression.
    Depositor
    Samuel McBrayer
    Article
    Sternisha et al Science. 2026 Jul 16;393(6808):eadx8675.
    Insert
    VP64-NtcA-2xNLS_SV40
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG and FLAG-mCherry-T2A
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    254310-plasmid-map-sequence-id-508529

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  13. pLenti EF1a mCherry-T2A-VP64-NtcA_R90E-2xNLS_SV40

    Plasmid
    #254311
    Purpose
    Constitutive expression of VP64- NtcA_R90E-2xNLS_SV40 for use in the inactive aKG-ON biosensor. Requires pLenti Neo glnA PCC 6803 reporter gene plasmid. mCherry to monitor NtcA fusion expression.
    Depositor
    Samuel McBrayer
    Article
    Sternisha et al Science. 2026 Jul 16;393(6808):eadx8675.
    Insert
    VP64-NtcA_R90E-2xNLS_SV40
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG and FLAG-mCherry-T2A
    Expression
    Mammalian
    Mutation
    NtcA R90E
    Promoter
    EF1a
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    254311-plasmid-map-sequence-id-508538

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  14. Flowcode_42

    Plasmid
    #231472
    Purpose
    Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    FLAG, OLLAS, and V5
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    231472-plasmid-map-sequence-id-461552

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  15. Flowcode_43

    Plasmid
    #231473
    Purpose
    Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    FLAG, S, and VSVg
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    231473-plasmid-map-sequence-id-461436

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  16. Flowcode_44

    Plasmid
    #231474
    Purpose
    Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    FLAG, S, and V5
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    231474-plasmid-map-sequence-id-461434

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  17. Flowcode_45

    Plasmid
    #231475
    Purpose
    Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    FLAG, V5, and VSVg
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    231475-plasmid-map-sequence-id-461383

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  18. Flowcode_56

    Plasmid
    #231486
    Purpose
    Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    FLAG, OLLAS, and S
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    231486-plasmid-map-sequence-id-461393

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  19. Flowcode_26

    Plasmid
    #231450
    Purpose
    Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    C, FLAG, and V5
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    231450-plasmid-map-sequence-id-461356

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  20. Flowcode_37

    Plasmid
    #231461
    Purpose
    Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    FLAG, HA, and OLLAS
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    231461-plasmid-map-sequence-id-461380

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Showing: 11961 - 11980 of 14795 results