We narrowed to 243 results for: Weissman Jonathan

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pMH0004

Plasmid

#174087

Purpose

UCOE-SFFV-Cas9-BFP

Depositor

Jonathan Weissman

Article

Hein et al Nat Biotechnol. 2022 Mar;40(3):391-401.

Insert

Cas9-BFP

Use

Lentiviral

Promoter

SFFV

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1663

Plasmid

#112037

Purpose

Gal-Estradiol transcription activation expression cassette marked with KanR

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

Gal DNA binding-estradiol binding-MSN2 activator

Expression

Yeast

Promoter

ADH1

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMH0007

Plasmid

#174162

Purpose

UCOE-EF1A-Cas9-BFP

Depositor

Jonathan Weissman

Article

Hein et al Nat Biotechnol. 2022 Mar;40(3):391-401.

Insert

Cas9-BFP

Use

Lentiviral

Promoter

EF1A

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1504

Plasmid

#62358

Purpose

RPS2 HA-TEV-AVI pop-in/pop-out plasmid

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

RPS2 fragment

Use

Yeast targeting

Tags

HA-TEV-AVI

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1665

Plasmid

#112039

Purpose

Integrating plasmid for expression of ER localized mCherry at TRP1 locus

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ER-mCherry

Expression

Yeast

Promoter

TPI

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1510

Plasmid

#62364

Purpose

pFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-mVenus-Ubc6 tail anchor

Expression

Yeast

Mutation

L232H mutation compared to standard mVenus

Promoter

Sec63

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1666

Plasmid

#112040

Purpose

CEN/ARS plasmid for GAL-inducible empty reporter V5-TEV-EGFP tagged expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

None

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1505

Plasmid

#62359

Purpose

RPL16a HA-TEV-AVI pop-in/pop-out plasmid

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

RPL16A

Use

Yeast tagging

Tags

HA-TEV-AVI

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1506

Plasmid

#62360

Purpose

RPL16b HA-TEV-AVI pop-in/pop-out plasmid

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

RPL16B

Use

Yeast targeting

Tags

HA-TEV-AVI

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1667

Plasmid

#112041

Purpose

CEN/ARS plasmid for GAL-inducible ERG5-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ERG5

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1660

Plasmid

#112034

Purpose

C-terminal tag with AID*(AtIAA17_71-114)-HA marked with LEU

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

IAA17

Tags

HA

Expression

Yeast

Mutation

only amino acids 71-114

Promoter

None

Available Since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1515

Plasmid

#62385

Purpose

pFA6a Osm1-yoEGFP::LEU2

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

OSM1-EGFP fusion

Tags

EGFP

Expression

Yeast

Promoter

Adh1p

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pMK1200

Plasmid

#84219

Purpose

Lentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherry

Depositor

Martin Kampmann, Jonathan Weissman

Article

Kampmann et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pENN496_miniCRISPRcharm_dSaCas9

Plasmid

#220837

Purpose

mini CRISPRcharm expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

mini CRISPRcharm

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSNR52-sgTET

Plasmid

#46923

Purpose

Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TRE elements of pTET07 promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgRNA targeting endogenous TRE elements of pTET07 promoter

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pJW1517

Plasmid

#62387

Purpose

pFA6a Osm1(M33A)-yoEGFP::LEU2

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

OSM1-EGFP fusion

Tags

EGFP

Expression

Yeast

Mutation

Methionine 33 mutated to Alanine

Promoter

Adh1p

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP

Plasmid

#188773

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pJW1514

Plasmid

#62384

Purpose

pCR2.1 linker-tev-linker-3X-FLAG::KanMX

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

tev 3x FLAG

Use

Yeast c-terminal tagging vector

Tags

linker-tev-linker-3xflag

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLG_GI4

Plasmid

#111595

Purpose

GI Library Vector

Depositor

Jonathan Weissman

Article

Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1670

Plasmid

#112044

Purpose

CEN/ARS plasmid for GAL-inducible OLE1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

OLE1

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

P319T

Promoter

GAL1

Available Since

May 28, 2022

Availability

Academic Institutions and Nonprofits only

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