Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 2,779 results for: GUS

Showing: 1181 - 1200 of 2779 results

Results per page:

Sort results by:

pEXSISERS_NLuc_FRT-PuroR

Plasmid

#177865

Purpose

Cloning Backbone for NanoLuc-luciferase-based EXSISERS containing FRT-sites flanked PuroR cassette

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

NLuc-based EXSISERS

Use

CRISPR, Synthetic Biology, and Unspecified

Tags

OLLAS

Expression

Mammalian

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEXSISERS_BSD_FRT-PuroR

Plasmid

#177867

Purpose

Cloning Backbone for Blasticidin-S-deaminase-based EXSISERS containing FRT-sites flanked PuroR cassette

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

BSD-based EXSISERS

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Tags

OLLAS

Expression

Mammalian

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEXSISERS_SurfaceHaloTag_FRT-PuroR

Plasmid

#177869

Purpose

Cloning Backbone for Surface-HaloTag-based EXSISERS containing FRT-sites flanked PuroR cassette; clone homology arms via BbsI (or BpiI).

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

Surface-HaloTag-based EXSISERS

Use

CRISPR, Synthetic Biology, and Unspecified

Expression

Mammalian

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCambia2300-Aar1-ccdB-GmCH

Plasmid

#254405

Purpose

Gateway-compatible destination vector for bifluorescence complementation (BiFC). The plasmid carries Gus-mCherry expressed from the Arabidopsis Ubiquitin 10 promoter.

Depositor

Maria Harrison

Article

Ivanov et al Science. 2024 Jan 26;383(6681):443-448.

Type

Empty backbone

Expression

Plant

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX209-neo-active WRN

Plasmid

#125788

Purpose

constitutive, ectopic expression of human active WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-active WRN (WRN Human)

Tags

5x myc

Expression

Mammalian

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX209-neo-WRN K577M

Plasmid

#125790

Purpose

constitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN K577M (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

K577M (helicase-dead)

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX209-neo-WRN E84A

Plasmid

#125789

Purpose

constitutive, ectopic expression of an exonuclease-dead mutant of human WRN (E84A)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN E84A (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

E84A (exonuclease-dead)

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSITEP-puro-shWRN2

Plasmid

#125785

Purpose

DOX-inducible expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN2 (WRN Human)

Use

RNAi

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-puro-shRPS6

Plasmid

#125780

Purpose

constitutive expression of a short-hairpin RNA targeting human RPS6 (RNAi positive control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shRPS6 (RPS6 Human)

Use

RNAi

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSITEP-puro-shWRN1

Plasmid

#125783

Purpose

DOX-inducible expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN1 (WRN Human)

Use

RNAi

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Multiplex-CRISPR-miR-200FAM-T2A-GFP

Plasmid

#179856

Purpose

to delete miR200 family genes including miR-141, miR-200c, miR-429 and miR-200b

Depositor

Brendan Jenkins

Article

Yu et al Oncogene. 2021 Oct 19. pii: 10.1038/s413

Insert

hsa-miR-200 family (miR-200b, miR-200a, miR-429, miR-141 and miR-200c ) (MIR141, MIR200B, MIR429, MIR200C, MIR200A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSITEP-puro-shWRN1-C911

Plasmid

#125784

Purpose

a seed-matched control for pRSITEP-puro-shWRN1

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN1-C911 (WRN Human)

Use

RNAi

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-puro-shWRN1

Plasmid

#125781

Purpose

constitutive expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN1 (WRN Human)

Use

RNAi

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSITEP-puro-shWRN2-C911

Plasmid

#125786

Purpose

a seed-matched control for pRSITEP-puro-shWRN2

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN2 (WRN Human)

Use

RNAi

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-puro-shWRN2

Plasmid

#125782

Purpose

constitutive expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN2 (WRN Human)

Use

RNAi

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_BRD003-sgWRN-EIJ

Plasmid

#125774

Purpose

constitutive expression of a guide RNA targeting an exon-intron junction of human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgWRN-EIJ (WRN Human)

Use

CRISPR

Available Since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_BRD003-sgWRN1

Plasmid

#125771

Purpose

constitutive expression of a guide RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgWRN1 (WRN Human)

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-puro-shPSMD2

Plasmid

#125779

Purpose

constitutive expression of a short-hairpin RNA targeting human PSMD2 (RNAi positive control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shPSMD2 (PSMD2 Human)

Use

RNAi

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_BRD003-sgWRN2

Plasmid

#125772

Purpose

constitutive expression of a guide RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgWRN2 (WRN Human)

Use

CRISPR

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_BRD051-sgCh2-2

Plasmid

#125775

Purpose

constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgCh2-2

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1181 - 1200 of 2779 results

Results per page:

Sort results by: