We narrowed to 9,129 results for: Pol

1. L1_UBE2:mS-Lt-Ck3

   Plasmid

   #136131

   Purpose

   L1 in position 3, mark cell outline with mScarletI

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpUBE2:mScarletI-Lti6b_3tNos-35S
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pKLBac-FluB-trimer

   Plasmid

   #229859

   Purpose

   Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

   Depositor

   Stephen Cusack

   Article

   Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

   Inserts

   PA, Influenza B (B/Memphis/13/03)
   PB1, Influenza B (B/Memphis/13/03)
   PB2, Influenza B (B/Los Angeles/1/02)
   

   Tags

   2x-Streptag and 8xHis-tag

   Expression

   Insect

   Available Since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-2T-RNAPII-CTD wt #14-HA

   Plasmid

   #160688

   Purpose

   Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

   Depositor

   Jeffry Corden

   Article

   Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

   Insert

   RNA Polymerase II CTD
   

   Expression

   Bacterial

   Mutation

   C terminal domain only

   Available Since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. L1_KanR-Ck1

   Plasmid

   #136123

   Purpose

   L1 in position 1 (next to LB in L2 construct), for KanR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpEF1a:nptII_3t35S
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pCLX-M38s/TOP-dGFP

   Plasmid

   #114295

   Purpose

   Expression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoter

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   dGFP
   

   Use

   Lentiviral

   Mutation

   destabilized GFP

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. L1_UBE2:mS-Lt-Ck4

   Plasmid

   #136132

   Purpose

   L1 in position 4, mark cell outline with mScarletI

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpUBE2:mScarletI-Lti6b_3tNos-35S
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. MCV-hVam6p-

   Plasmid

   #32059

   Depositor

   Patrick Moore

   Article

   Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

   Insert

   MCV-hVam6p-
   

   Mutation

   TG to GC mutations at 3280-3281 (nt) of full cons…

   Promoter

   none from vector. Insert contains native promoter…

   Available Since

   Oct. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLS-CMV-mP-luc

   Plasmid

   #213506

   Purpose

   Positive control vector for luciferase lentivirus-based enhancer assay

   Depositor

   Nadav Ahituv

   Article

   Deng et al Science. 2024 May 24;384(6698):eadh0559.

   Insert

   Luciferase
   

   Use

   Lentiviral

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJB53

   Plasmid

   #226309

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with V(T/S)G to AAA CsoS2 MR1-3
   

   Expression

   Bacterial

   Mutation

   VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

   Promoter

   pTRC

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PROM5_Mprnpo_chlo

    Plasmid

    #163951

    Purpose

    L0 part - promoter

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Marchantia polymorpha chloroplast DNA-dependent RNA polymerase operon promoter (rpo) (genome position: 5595-5739)
    

    Expression

    Bacterial

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. L1_UBE2:eG-Lt-Ck4

    Plasmid

    #136130

    Purpose

    L1 in position 4, mark cell outline with eGFP

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpUBE2:eGFP-Lti6b_3tNos-35S
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. L1_NOP1:mS-N7-Ck2

    Plasmid

    #136126

    Purpose

    L1 in position 2, example of weak promoter

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpNOP1:mScarletI-N7_3tNos-35S
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. L1_UBE2:mT-N7-Ck2

    Plasmid

    #136127

    Purpose

    L1 in position 2, control of expression

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpUBE2:mTurquoise2-N7_3tNos-35S
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. L1_UBE2:mT-N7-Ck3

    Plasmid

    #136128

    Purpose

    L1 in position 3, control of expression

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpUBE2:mTurquoise2-N7_3tNos-35S
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. L1_UBE2:eG-Lt-Ck3

    Plasmid

    #136129

    Purpose

    L1 in position 3, mark cell outline with eGFP

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpUBE2:eGFP-Lti6b_3tNos-35S
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKAR2-Br512_Mut235

    Plasmid

    #179278

    Purpose

    Expresses Br512_Mut235 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

    Insert

    Engineered Bst DNA Polymerase I Large Fragment
    

    Tags

    8x His tag; Villin Headpiece 47 amino acid (HP47)…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQE-WT-RGVG-M6

    Plasmid

    #70172

    Purpose

    Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

    Depositor

    Andrew Ellington

    Article

    Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

    Insert

    RGVG-M6 T7 RNA polymerase
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T5/Lac

    Available Since

    Dec. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. L1_lacZgRNA-Ck3

    Plasmid

    #136136

    Purpose

    L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpU6:lacZgRNA
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. L1_Cas9-Ck4

    Plasmid

    #136135

    Purpose

    L1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR vectors

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpEF1a:Cas9_3tNos-35S
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LentiGuide-Neo

    Plasmid

    #139449

    Purpose

    Lentiviral vector with gRNA scaffold and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    no sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge