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p-eGFP-beta 2 CP

Plasmid

#13298

Depositor

John Cooper

Article

Schafer et al J Cell Biol. 1998 Dec 28. 143(7):1919-30

Insert

capping protein beta 2 subunit (Capzb Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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MAC3-tag-C

Plasmid

#185481

Purpose

Gateway compatible destination vectors containing StrepIII/HA/UltralD

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Type

Empty backbone

Tags

StrepIII-HA-UltraID

Expression

Bacterial and Mammalian

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pHIV-NAT-FLAG-CIP2A FL (sg2R)

Plasmid

#222624

Purpose

Lentiviral vector that expresses Flag-tagged sgRNA resistant CIP2A in mammalian cells

Depositor

Daniel Durocher

Article

Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

Insert

CIP2A (CIP2A Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF-1-alpha promoter

Available Since

July 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MSCVhygro-F-G9a

Plasmid

#41721

Purpose

Retroviral expression of human G9a in mammalian cells

Depositor

Kai Ge

Article

Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

Insert

G9a (EHMT2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-LCK

Plasmid

#23890

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LCK (LCK Human)

Use

Gateway donor vector

Mutation

V93I

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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mNeongreen-PXN

Plasmid

#129604

Purpose

In vivo visualization of focal adhesions (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Paxillin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGBSedit Sham

Plasmid

#223200

Purpose

Group B Streptococcus Efficient Genome Editing with Modular crRNA

Depositor

Thomas Hooven

Article

Hillebrand et al Mol Microbiol. 2025 Nov;124(5):449-461.

Insert

N/A

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFp10

Plasmid

#170460

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Article

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

none

Expression

Insect

Promoter

dHsp70

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF2058

Plasmid

#141898

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NKX2-2 (NKX2-2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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ancBE4-SpRY

Plasmid

#226855

Purpose

Expresses ancBE4max-SpRY base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

ancBE4max-SpRY

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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sFlt1-e15a V5

Plasmid

#86045

Purpose

human Soluble Flt1 isoform e15a (terminates in exon 15a)

Depositor

Christie Thomas

Article

Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

Insert

human soluble Flt1 isoform e15a (FLT1 Human)

Tags

6xHis and V5

Expression

Mammalian

Mutation

isoform e15a (terminates in exon 15a)

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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MIP 227 CoMiP 4in1 wo shRNA p53

Plasmid

#63727

Purpose

To express Oct4, Klf4, Sox2, and c-Myc. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

Depositor

Mark Kay, Joseph Wu

Article

Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

Insert

Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC Human)

Tags

IRES-dtomato

Expression

Mammalian

Mutation

codon-optimised

Promoter

SFFV

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI

Plasmid

#117006

Purpose

Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMXSIP-FLAG-ATPIF1

Plasmid

#204703

Purpose

expresses FLAG-ATPIF1 in mammalian cells

Depositor

William Kaelin

Article

Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

Insert

ATP synthase inhibitory factor subunit 1 (ATP5IF1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAVEC2_C-TurboID

Plasmid

#231301

Purpose

For transiently expressing fusion proteins with C terminal TurboID for proximity labeling in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

TurboID

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-hXRCC1-mCherry-Hygro

Plasmid

#176532

Purpose

mCherry fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.

Insert

X-Ray Repair Cross Complementing 1 (XRCC1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-eDHFR-MCS

Plasmid

#172108

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR

Depositor

Shinya Tsukiji

Article

Ishida et al J Am Chem Soc. 2013 Aug 28;135(34):12684

Insert

EGFP-eDHFR-20aa (folA E. coli)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF0490

Plasmid

#143030

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZSCAN4 (ZSCAN4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-mCherry-P2A-Cre-WPRE

Plasmid

#107312

Purpose

Encoding hSyn-mCherry-Cre

Depositor

Hui Yang

Article

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Has Service

AAV1

Insert

hSyn-mCherry-P2A-Cre-WPRE

Use

AAV

Expression

Mammalian

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-P2A-HSA

Plasmid

#121936

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

dCas9-P2A-HSA

Use

CRISPR and Lentiviral

Tags

HSA

Expression

Mammalian

Promoter

CMV-TO

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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