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pCMV-PuroR-T2A-eGFP

Plasmid

#234734

Purpose

Puromycin resistance/eGFP cassette in mammalian cells (transfection marker)

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PuroR-T2A-eGFP

Expression

Mammalian

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAGM47523

Plasmid

#153221

Purpose

Level 0 zCas9i vector

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

SpCas9 with introns and 2 NLS

Use

Synthetic Biology

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6-H2B-mCherry

Plasmid

#89766

Purpose

Lentivirus destination vector for stable expression of mCherry-tagged histone H2B

Depositor

Torsten Wittmann

Article

Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

Insert

Histone H2B (H2BC11 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCW-Tet-Cas9-BFP-PGK-Blast

Plasmid

#196720

Purpose

Tet-inducible expression of Cas9-T2A-TagBFP

Depositor

Bernhard Schmierer

Insert

Cas9-T2A-TagBFP

Use

CRISPR and Lentiviral

Tags

3xFLAG-SV40NLS and Nucleoplasmin NLS

Promoter

TRE, PGK

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-YAP1-shRNA1

Plasmid

#176846

Purpose

lentiviral vector for doxycycline-inducible human YAP1 knockdown

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

YAP1-Sh1 (YAP1 Human)

Use

Lentiviral

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-hYAP1

Plasmid

#17843

Depositor

Marius Sudol

Article

Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

Insert

YAP (YAP1 Human)

Tags

EGFP

Expression

Bacterial and Mammalian

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

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pscAAV-CAG-GFP

Plasmid

#83279

Purpose

recombinant AAV vector packaging self-complementary GFP under the CAG promoter

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

enhanced green fluorescent protein

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pNP2390 [pNP2322 - ISCro4 Donor + ISCro4 Target-RTG-Matched]

Plasmid

#247252

Purpose

Measure ISCro4 activity via mCherry expression

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

pNP2322 - ISCro4_Donor, ISCro4_Target_RTG-Matched

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1 - TRC control

Plasmid

#10879

Purpose

Negative control shRNA vector containing non-hairpin insert.

Depositor

David Root

Article

Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

Insert

non-hairpin 18bp

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pBAD-ApplePy1Lowest

Plasmid

#239057

Purpose

Bacterial expression of lowest affinity red fluorescent pyruvate biosensor ApplePy1Lowest

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

ApplePy1Lowest

Expression

Bacterial

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD42-BtuCEDFB

Plasmid

#237541

Purpose

plasmid encodes BtuC, BtuD, BtuE, BtuF, BtuB for enhanced solubilization of cobalamin proteins

Depositor

Squire Booker

Article

Lanz et al Biochemistry. 2018 Mar 6;57(9):1475-1490

Inserts

btuB
btuF
btuCED

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Hygro

Plasmid

#139462

Purpose

Lentiviral vector with gRNA scaffold and hygromycin selectable marker

Depositor

Caroline Goujon

Insert

no sgRNA inserted; resistance gene: hygroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-GfaABC1D-MCS--4x6T-WPRE

Plasmid

#196417

Purpose

Empty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV; Astrocyte-selective

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau

Plasmid

#46904

Purpose

expresses EGFP tagged Tau in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

MAPT (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

This WT htau construct contains human four-repeat…

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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GALT-SEP-mRuby3

Plasmid

#200938

Purpose

trans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

Depositor

Nir Hacohen

Article

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Inserts

GalT (B4GALT1 Human)
SEP-mRuby3

Use

Lentiviral

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-tdTomato

Plasmid

#83029

Purpose

expresses tdTomato fluorescent protein under the CAG promoter

Depositor

Angelique Bordey

Article

Pathania et al PLoS One. 2012;7(5):e38174. doi: 10.1371

Insert

tdTomato

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCW57-MCS1-P2A-MCS2 (Hygro)

Plasmid

#80922

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-ABE N-terminal

Plasmid

#137177

Purpose

AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-ABE N-terminal

Use

AAV

Mutation

Cas9 D10A

Promoter

Cbh

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX_DSB_Spectrum-V3

Plasmid

#192476

Purpose

Lentiviral DSB Spectrum V3 Reporter System

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, mCherry, incompleteGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8s

Plasmid

#162371

Purpose

Mammalian expression of ultrafast protein calcium sensor

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8s

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

CMV

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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