163,486 results

1. CMV-Mito4x-GCaMP6f

   Plasmid

   #127870

   Purpose

   Fluorescent reporter for mitochondrial matrix Ca2+

   Depositor

   Timothy Ryan

   Article

   Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

   Insert

   4 repeats of COX8 signal peptide plus GCaMP6f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. N1-Tq-Ca-FLITS

   Plasmid

   #191454

   Purpose

   Genetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   Tq-Ca-FLITS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSP2N2

   Plasmid

   #29520

   Depositor

   Stephen Sligar

   Article

   Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

   Insert

   MSP2N2
   

   Use

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   tandem MSP; His-tag on N-terminus followed by spa…

   Promoter

   Available Since

   May 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-GFP-LC3-RFP-LC3ΔG

   Plasmid

   #168997

   Purpose

   Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

   Plasmid

   #210525

   Purpose

   AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   mtagBFP-KASH
   U6-gRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p10xUAS-luc2

   Plasmid

   #173006

   Purpose

   firefly luciferase reporter vector with 10x clustered UAS binding sites

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   10xUAS-luc2
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-NLS-HA-Bxb1

   Plasmid

   #51271

   Purpose

   Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   NLS-HA-Bxb1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX_307

   Plasmid

   #41392

   Purpose

   Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

   Depositor

   David Root

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTargetF

   Plasmid

   #62226

   Purpose

   Constitutive expression of sgRNA without donor editing template DNA

   Depositor

   Sheng Yang

   Article

   Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pij23119

   Available Since

   March 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. JAK2 (V617F)-pcw107-V5

    Plasmid

    #64610

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    JAK2 (JAK2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    V617F

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. CROPseq-Guide-Puro

    Plasmid

    #86708

    Purpose

    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

    Depositor

    Christoph Bock

    Article

    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n

    Insert

    EF1a-Puro-WPRE-hU6-gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-Gsdmd-NT

    Plasmid

    #80951

    Purpose

    Expresses Gsdmd-NT in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

    Insert

    Gsdmd-NT (Gsdmd Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. AICSDP-15:ACTB-mEGFP

    Plasmid

    #87425

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    ACTB Homology Arms with mEGFP-linker (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    March 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. iCas

    Plasmid

    #84232

    Purpose

    Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

    Depositor

    Meng How Tan

    Article

    Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    2A-OFP co-expression and ERT2-ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDIRECT_22C

    Plasmid

    #91135

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-ExRai-AMPKAR

    Plasmid

    #192446

    Purpose

    Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. SF-SON IRES Puro

    Plasmid

    #214402

    Purpose

    Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)

    Depositor

    Tanja Kuhlmann

    Article

    Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

    Plasmid

    #60903

    Purpose

    Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    Cas9 dead
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. psfaMs-Tn5

    Plasmid

    #183637

    Purpose

    Expresses anti-mouse nanobody fused with Tn5

    Depositor

    Goncalo Castelo-Branco

    Article

    Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

    Insert

    anti mouse secondary nanobody fused to Tn5
    

    Use

    Tags

    anti-mouse nanobody

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW57-MCS1-P2A-MCS2 (Hygro)

    Plasmid

    #80922

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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