165,666 results

1. pAF0466 Ef1a-Nm60_attB acceptor

   Plasmid

   #193476

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Nm60_attB
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-WASH1-N-18

   Plasmid

   #55163

   Purpose

   Localization: Cytoskeleton, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   WASH1
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pEAK10-His-Myc-Kal7

   Plasmid

   #25454

   Depositor

   Betty Eipper

   Article

   Penzes et al J Biol Chem. 2000 Mar 3. 275(9):6395-403

   Insert

   Kal7
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   July 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPR v2-sgDHODH-4

   Plasmid

   #186022

   Purpose

   knock out DHODH in mammalian cells

   Depositor

   Boyi Gan

   Article

   Mao et al Nature. 2021 May;593(7860):586-590. doi:

   Insert

   Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET30b-5M SrtA with TEV site

   Plasmid

   #86962

   Purpose

   Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

   Depositor

   Ron Bose

   Article

   Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

   Insert

   S.aureus SrtA 5M
   

   Tags

   6x Histidine tag and TEV site

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T

   Promoter

   T7

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA

   Plasmid

   #216319

   Purpose

   Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   Split Cerulean + splice acceptor site
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA

   Plasmid

   #216318

   Purpose

   Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   split cerulean fluorescent protein + splice donor site
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIA1414

   Plasmid

   #172402

   Purpose

   Expression of SARS-COV-2 Nsp9 in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

   Insert

   SARS-COV-2 Nsp9 (ORF1ab Synthetic)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ubi:ccdb-eGFP + RUBY

   Plasmid

   #233310

   Purpose

   GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and RUBY marker

   Depositor

   Thomas Baum

   Insert

   Ubi:ccdb-eGFP + RUBY
   

   Use

   Gateway destination

   Tags

   eGFP

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ubi:ccdb + RUBY

    Plasmid

    #233306

    Purpose

    GATEWAY compatible vector with soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of ccdb selection toxin and RUBY marker

    Depositor

    Thomas Baum

    Insert

    Ubi:ccdb + RUBY
    

    Use

    Gateway destination

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPR v2-sgDHODH-2

    Plasmid

    #186023

    Purpose

    knock out DHODH in mammalian cells

    Depositor

    Boyi Gan

    Article

    Mao et al Nature. 2021 May;593(7860):586-590. doi:

    Insert

    Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_GR-RE_MLPmin_BC1094-luc2

    Plasmid

    #227114

    Purpose

    Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1094

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered GR response element linked to MLP minimal promoter driving barcode 1094 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXint129

    Plasmid

    #178208

    Purpose

    Encodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.

    Depositor

    Gregory Phillips

    Article

    Platt et al Plasmid. 2000 Jan . 43(1):12-23.

    Insert

    xis excisionase, int integrase (xis, lambdap32 Bacteriophage lambda)
    

    Expression

    Bacterial

    Promoter

    lacI

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pN1-COX8-mScarletI

    Plasmid

    #129341

    Purpose

    Mitochondria matrix localization

    Depositor

    Yasushi Okada

    Insert

    mScarlet(i) (Addgene 85044)
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe zeo HMGA2

    Plasmid

    #17411

    Depositor

    Tyler Jacks

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

    Insert

    HMGA2 (Hmga2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-myc-EFNB2 (WT)

    Plasmid

    #200975

    Purpose

    Mammalian expression plasmid for wild type, myc-tagged EFNB2

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EFNB2 (EFNB2 Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW57.1_V5_APEX_FLAG_Ct

    Plasmid

    #194069

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG-APEX-V5

    Expression

    Mammalian

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.1 Bach1 S990A

    Plasmid

    #73643

    Purpose

    Mammalian expression of Bach1 S990A mutant (BRCA binding mutant).

    Depositor

    Sharon Cantor

    Insert

    Bach1 (BACH1 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    S990A (BRCA binding mutant)

    Promoter

    CMV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

19. pLB038 - PHS1∆P

    Plasmid

    #136285

    Purpose

    entry vector for GreenGate cloning method, C module, open reading frame of phosphatase-inactive PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1) (PHS1DP)

    Depositor

    Alexis Maizel

    Article

    Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

    Insert

    PHS1∆P
    

    Expression

    Bacterial

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPOTv7-blast-3HA

    Plasmid

    #221048

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    3xHA epitopes
    

    Use

    Unspecified

    Promoter

    read through

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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