165,666 results

1. Ubi:ccdb + mcherry

   Plasmid

   #233303

   Purpose

   GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of ccdb selection toxin and mCherry marker

   Depositor

   Thomas Baum

   Insert

   Ubi:ccdb + mcherry
   

   Use

   Gateway destination

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ubi:ccdb-3xHA + mcherry

   Plasmid

   #233302

   Purpose

   GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of 3xHA tag paired with ccdb selection toxin and mCherry marker

   Depositor

   Thomas Baum

   Insert

   Ubi:ccdb-3xHA + mcherry
   

   Use

   Gateway destination

   Tags

   3xHA

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ubi:ccdb-eGFP + mcherry

   Plasmid

   #233300

   Purpose

   GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and mCherry marker

   Depositor

   Thomas Baum

   Insert

   Ubi:ccdb-eGFP + mcherry
   

   Use

   Gateway destination

   Tags

   eGFP

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. AiP12286 - pAAV-AiE0078m-minBG-iCre-WPRE-HGHpA (Alias: CN2286)

   Plasmid

   #230360

   Purpose

   AiE0078m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre
   

   Use

   AAV

   Promoter

   minBG

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgPPP1R2

   Plasmid

   #227391

   Purpose

   Adhesion module, guide RNA recognizes PPP1R2

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   guide RNA targeting PPP1R2
   

   Use

   Lentiviral

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACYC-yhaM-RFP

   Plasmid

   #207651

   Purpose

   Overexpression of native L-cysteine desulfidase and RFP in E. coli MG1655

   Depositor

   Benjamin Woolston

   Article

   Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

   Insert

   yhaM
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUdOm7.1

   Plasmid

   #210287

   Purpose

   Empty backbone with zeocin resistance for high expression in mammalian cells

   Depositor

   Corrie daCosta

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRS-dCas9

   Plasmid

   #220193

   Purpose

   mini-Tn7 dCas9 expression vector

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Pseudomonas aeruginosa codon optimized

   Promoter

   promoterless

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4046749

   Plasmid

   #145629

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp11

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. Cytoplasmic PancACe

    Plasmid

    #215708

    Purpose

    Expresses acetyl-CoA biosensor in the cytoplasm

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and cytoplasmic localization sequence

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #71236

    Purpose

    Express sgRNA and dCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A Puro
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRIS-PITChv2-dTAG-Puro (BRD4)

    Plasmid

    #91796

    Purpose

    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    FKBP_F36V-2xHA-P2A-Puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tet-pLKO-puro-Scrambled

    Plasmid

    #47541

    Purpose

    Tet inducible scrambled shRNA.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    Scrambled shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. sortase A pentamutant (eSrtA) in pET29

    Plasmid

    #75144

    Purpose

    evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

    Depositor

    David Liu

    Article

    Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

    Insert

    sortase A pentamutant
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T. Deleted amino a…

    Promoter

    T7

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRFHUE-eGFP

    Plasmid

    #89469

    Purpose

    GFP labelling of filamentous fungi by Agrobacterium tumefaciens mediated transformation

    Depositor

    Luis González-Candelas

    Article

    Crespo-Sempere et al Int J Food Microbiol. 2011 Aug 2;148(2):

    Insert

    eGFP
    

    Use

    Binary plasmid for atmt of filamentous fungi

    Promoter

    A. nidulans gpdA

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV R-CEPIA1er

    Plasmid

    #58216

    Purpose

    Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    R-CEPIA1er
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGTM_YR375_SUMO_Protease_001

    Plasmid

    #190063

    Purpose

    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    ULP1_YEAST (ULP1 Budding Yeast)
    

    Tags

    8X His-MBP (Maltose Binding Protein)

    Expression

    Bacterial

    Mutation

    Construct contains residues 347 – 621

    Promoter

    T7 lac

    Available Since

    Jan. 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pRL056 pDESTattB_exorh:EGFP

    Plasmid

    #213510

    Purpose

    attB-containing pDEST plasmid, for use in Multisite Gateway reaction

    Depositor

    Christian Mosimann

    Article

    Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV2-SEP-GluA1 (M1)

    Plasmid

    #64942

    Purpose

    mammalian expression of rat GluA1

    Depositor

    Jeremy Henley

    Article

    Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

    Insert

    GluA1 (Gria1 Rat)
    

    Tags

    SEP (superecliptic pHluorin)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-2xFYVE

    Plasmid

    #140047

    Purpose

    Expresses GFP-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available Since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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