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Plasmid#171075PurposeExpresses Gal4-vp16 fusion protein in bacteriaDepositorInsertGal4 DBD (NEWENTRY Budding Yeast)
UseTagsHis and VP16 AD (413-490)ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-Rac1-wt
Plasmid#12977DepositorInsertRac1 (RAC1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hATG7wt
Plasmid#87867PurposeExpress a wild type human Atg7/Apg7 in mammalian cellsDepositorInsertATG7 (ATG7 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)
Plasmid#160626PurposeTU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domainsDepositorInsertP35s-dCas9:TV-Tnos
UseTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF2671
Plasmid#142972PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNEUROD4 (NEUROD4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-LRPAP1
Plasmid#31327DepositorInsertlow density lipoprotein receptor-related protein associated (LRPAP1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-28:TUBA1B-mTagRFP-T
Plasmid#101785PurposeHomology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1BDepositorInsertTUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFLAG-CMV-hErk1(K71R)
Plasmid#49329PurposeExpresses kinase deficient FLAG-tagged human Erk1DepositorInsertErk1 (MAPK3 Human)
UseTagsFLAGExpressionMammalianMutationK71RPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-26:ST6GAL1-mEGFP
Plasmid#101786PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human ST6GAL1DepositorInsertST6GAL1 Homology Arms with linker-mEGFP (ST6GAL1 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMAH2-CageCys
Plasmid#71404PurposeExpresses tRNA and synthetase for mammalian encoding of a photocaged cysteineDepositorInsertaminoacyl-tRNA synthetase for a caged cysteine
UseTagsExpressionMutationPromoterCMVAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
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Cre Shine
Plasmid#37404PurposeThis is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.DepositorInsertCre Shine
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHBFD1Bad
Plasmid#53564PurposeProduces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinoseDepositorInserthbfd1 cDNA
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP2-NbALFA
Plasmid#201486PurposeBRET2 detection of ALFA-tagged proteinDepositorInsertGFP2-NbALFA
UseTagsGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
TMEM106B-EGFP
Plasmid#210366PurposeExpresses TMEM106B fused with EGFP in mammalian cellsDepositorInsertTMEM106B (TMEM106B Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-39:RAB5A-mEGFP
Plasmid#107579PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human RAB5ADepositorInsertRAB5A Homology Arms with mEGFP-linker (RAB5A Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMay 9, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTEC18
Plasmid#30177DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagsEBFP2ExpressionMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
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TFORF2886
Plasmid#143655PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGLI3 (GLI3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:YFP:Tnos (GB0209)
Plasmid#68219PurposeTranscriptional unit for Yellow Flurescent Protein plant expression driven by the 35S promoterDepositorInsertYFP
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only