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pGlnA-Ypet

Plasmid

#98278

Purpose

mammalian expression of GlnA-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

GlnA

Tags

Ypet

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pMUH_unc84_tdTFlag

Plasmid

#46024

Depositor

Sean Eddy

Article

Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

Insert

UNC-84 (unc-84 )

Use

10xuas-phi31-expression vector

Tags

tdTomato

Mutation

Two amino acid mutations (F279L and L500P) compar…

Promoter

10XUAS

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pOG231

Plasmid

#17736

Depositor

Geoff Wahl

Article

O'Gorman et al Proc Natl Acad Sci U S A. 1997 Dec 23;94

Insert

NLS-Cre recombinase

Use

Cre/Lox

Expression

Bacterial

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.nucGAP

Plasmid

#78736

Purpose

Expression of Ca2+ sensor GAP in the nucleus

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

nucGAP

Tags

nucleoplasmin (Xenopus)

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pJYDN2

Plasmid

#162454

Purpose

Yeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Type

Empty backbone

Use

Shuttle vector bacteria/yeast

Tags

DnbALFA nanobody and Ha and myc

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-LMP2A-IRES-GFP

Plasmid

#220352

Purpose

Expresses EBV latent gene LMP2A in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP2A (LMP-2A Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pDUAL CLDN (GFP)

Plasmid

#86981

Purpose

Lentiviral expression construct encoding Claudin-1 and GFP from separate promoters

Depositor

Joe Grove

Insert

CLDN1 (CLDN1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV/hPGK

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTK299

Plasmid

#59568

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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mEmerald-Occludin-C-14

Plasmid

#54211

Purpose

Localization: Tight Junctions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Occludin (OCLN Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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EGFP-alphasynuclein-WT

Plasmid

#40822

Depositor

David Rubinsztein

Article

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Insert

SNCA (SNCA Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pJYDN2p

Plasmid

#162455

Purpose

Positive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Insert

Aga2p-DnbALFA (AGA2 )

Use

Shuttle vector bacteria/yeast

Tags

DnbALFA nanobody and HA and myc

Promoter

GAL1

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp2

Plasmid

#163587

Purpose

Expresses BMP-2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp2 (Bmp2 Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-PFKL

Plasmid

#23729

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PFKL (PFKL Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223 CASP1[CARD]-mSG2 (inflammasome reporter)

Plasmid

#228043

Purpose

DONR vector for inflammasome speck reporter, designed from PMC9623368.

Depositor

Wade Harper

Insert

CASP1[CARD]-mSG2

Use

Gateway donr

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pTDpelB-CTwinStrep

Plasmid

#45941

Purpose

use to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequence

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Tags

PelB and Twin-Strep

Expression

Bacterial

Promoter

Trc (lactose/IPTG inducible)

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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p11U1ADb

Plasmid

#27384

Depositor

David Bartel

Article

Shechner et al Science. 2009 Nov 27. 326(5957):1271-5.

Insert

U1A A1-98 Y31H/Q36R (SNRPA Human)

Expression

Bacterial

Mutation

A1-98 Y31H/Q36R double mutant

Available Since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only
pGL2-200bp

Plasmid

#16293

Depositor

Wafik El-Deiry

Article

Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

Insert

p53 promoter fragment (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

fragment -188 to +12 relative to first transcript…

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC46A3

Plasmid

#132215

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC46A3 (SLC46A3 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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mCerulean-C1

Plasmid

#54592

Purpose

Localization: C1 Cloning Vector, Excitation: 433, Emission: 475

Depositor

Michael Davidson, Dave Piston

Article

Rizzo et al Nat Biotechnol. 2004 Apr . 22(4):445-9.

Type

Empty backbone

Tags

mCerulean

Expression

Mammalian

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
FASTHDR-Cterm-NanoLuc-Puromycin

Plasmid

#167209

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

NanoLuc

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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