170,492 results
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Plasmid#250354PurposeExpresses tandem BRCT domain from MCPH1 fused to eGFP. Can be used to detect yH2AX at DNA damage sites.DepositorInsertMCPH1 tandem BRCT
TagsNLS, eGFP and biotintag, TEV cleavageExpressionMammalianPromoterCAGGSAvailable SinceFeb. 10, 2026AvailabilityAcademic Institutions and Nonprofits only -
LSL-Cas9-Rosa26TV
Plasmid#61408PurposeCre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.DepositorInsertsCas9
EGFP
UseCRISPR, Cre/Lox, and Mouse TargetingTags3xFLAG and P2AExpressionMammalianMutationhuman codon optimizedPromoterCAGAvailable SinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNK3071
Plasmid#219755PurposeMoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.DepositorInsertmcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
ExpressionPlantAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4-UPRE-luc2P-Hygro
Plasmid#101788PurposeLuciferase reporter plasmid containing three tandem repeats of the unfolded protein response element (UPRE)DepositorInsert3xUPRE
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterminimal TATA-box promoter with low basal activityAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRL_SRSF2_WT_mCherry
Plasmid#84020Purposeexpression of SRSF2 in mammalian cellsDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTags2TA-mCherry and FlagExpressionMammalianPromoterPGKAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLCAR-CD19-28z
Plasmid#135991PurposeModular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralExpressionMammalianPromoterEF1a-shortAvailable SinceJune 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
CLYBL_Bxb1-GA_LP_Dual_TC
Plasmid#229776PurposeCLYBL targeting construct with the Bxb1-GA landing pad cassette.DepositorInsertFRT-mScarlet-attP(GA)-PuroR-F3
UseSynthetic BiologyExpressionMammalianPromoterpGKAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMV_AA050
Plasmid#216107PurposegDNA expression for Cas9 with ChenF+E trRNA (guide only)DepositorInsertgDNA expression for Cas9 with ChenF+E trRNA
UseCRISPR and Lentiviral; Assembled vectorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-DTR-GFP
Plasmid#124364Purposeconditional expression of a diphtheria toxin receptor (DTR)–GFP fusion proteinDepositorHas ServiceAAV2InsertDTR-GFP
UseAAVTagsGFPPromoterChicken B-actinAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2
Plasmid#132775PurposePrime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3bDepositorInsertPE2
TagsSV40 NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
ABE8e-N108Q
Plasmid#194157PurposeMammalian expression of ABE8e-N108QDepositorInsertTadA-8e (N108Q)-nSpCas9
UseCRISPRTags6*HisExpressionMammalianAvailable SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VSVG
Plasmid#64084PurposeExpression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein geneDepositorInsertVSV glycoprotein
ExpressionMammalianPromoterCAGAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-CRBN-midi
Plasmid#215330PurposeCRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader designDepositorInsertCRBN (CRBN Human)
Tags6xHis and TEVExpressionBacterialMutationcontains amino acids 41-187 followed by a Gly-Ser…PromoterlacIAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-hVAP-B
Plasmid#104448PurposeExpression of human VAP-B fused to GFP in mammalian cellsDepositorInsertvesicle-associated membrane protein-associated protein B (VAPB Human)
TagsEGFP and HAExpressionMammalianAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
L4552 x6NFATRE (NFAT) Ben DsRed-Express2 in TUPV1
Plasmid#244201PurposeExpression of a DsRed-Express2 reporter under an NFAT-responsive promoterDepositorInsertNFAT-responsive promoter (6 NFAT binding sites + YB_TATA), dsRed-Express2 reporter
UseSynthetic BiologyExpressionMammalianAvailable SinceOct. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDonor MCS Rosa26
Plasmid#37200DepositorInsertsRosa26 left targeting arm
MCS
Rosa26 right targeting arm
UseGenomic targeting - zinc fingerAvailable SinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMRX-No-HaloTag7-mGFP-LC3-mRFP
Plasmid#184902PurposeStable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic fluxDepositorInsertLC3B
TagsHaloTag7, monomeric EGFP, and monomeric RFPExpressionMammalianAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX333
Plasmid#64073PurposeVector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneTags3XFLAG-Cas9ExpressionMammalianPromoterCBh; U6Available SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Puro-P2A-EGFP
Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCh-Rab7A
Plasmid#61804PurposeHuman Rab7A fluorescently tagged with mCh on the N-terminus for mammalian expressionDepositorAvailable SinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only