Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pcDNA3_NSlmb-vhhGFP4 Citations (16)

Originally described in: Fluorescent fusion protein knockout mediated by anti-GFP nanobody.
Caussinus E, Kanca O, Affolter M Nat Struct Mol Biol. 2011 Dec 11;19(1):117-21. doi: 10.1038/nsmb.2180.
PubMed Journal

Articles Citing pcDNA3_NSlmb-vhhGFP4

Articles
Dynamics of CDKN1A in Single Cells Defined by an Endogenous Fluorescent Tagging Toolkit. Stewart-Ornstein J, Lahav G. Cell Rep. 2016 Feb 10. pii: S2211-1247(16)30023-7. doi: 10.1016/j.celrep.2016.01.045. PubMed
Protein Inactivation by Optogenetic Trapping in Living Cells. Park H, Lee S, Heo WD. Methods Mol Biol. 2016;1408:363-76. doi: 10.1007/978-1-4939-3512-3_25. PubMed
A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans. Wang S, Tang NH, Lara-Gonzalez P, Zhao Z, Cheerambathur DK, Prevo B, Chisholm AD, Desai A, Oegema K. Development. 2017 Jun 15. pii: dev.150094. doi: 10.1242/dev.150094. PubMed

Associated Plasmids

A Method for the Acute and Rapid Degradation of Endogenous Proteins. Clift D, McEwan WA, Labzin LI, Konieczny V, Mogessie B, James LC, Schuh M. Cell. 2017 Nov 13. pii: S0092-8674(17)31255-2. doi: 10.1016/j.cell.2017.10.033. PubMed

Associated Plasmids

Sculpting ion channel functional expression with engineered ubiquitin ligases. Kanner SA, Morgenstern T, Colecraft HM. Elife. 2017 Dec 19;6. pii: 29744. doi: 10.7554/eLife.29744. PubMed
Conditional control of fluorescent protein degradation by an auxin-dependent nanobody. Daniel K, Icha J, Horenburg C, Muller D, Norden C, Mansfeld J. Nat Commun. 2018 Aug 17;9(1):3297. doi: 10.1038/s41467-018-05855-5. PubMed

Associated Plasmids

Delete_zGrad is a nanobody-based degron system that inactivates proteins in zebrafish. Yamaguchi N, Colak-Champollion T, Knaut H. Elife. 2019 Feb 8;8. pii: 43125. doi: 10.7554/eLife.43125. PubMed
Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins. Yu D, Lee H, Hong J, Jung H, Jo Y, Oh BH, Park BO, Heo WD. Nat Methods. 2019 Nov;16(11):1095-1100. doi: 10.1038/s41592-019-0592-7. Epub 2019 Oct 14. PubMed
Optogenetic regulation of endogenous proteins. Redchuk TA, Karasev MM, Verkhusha PV, Donnelly SK, Hulsemann M, Virtanen J, Moore HM, Vartiainen MK, Hodgson L, Verkhusha VV. Nat Commun. 2020 Jan 30;11(1):605. doi: 10.1038/s41467-020-14460-4. PubMed

Associated Plasmids

Collectively stabilizing and orienting posterior migratory forces disperses cell clusters in vivo. Lin B, Luo J, Lehmann R. Nat Commun. 2020 Sep 8;11(1):4477. doi: 10.1038/s41467-020-18185-2. PubMed
Optogenetic Activation of Intracellular Nanobodies. Yu D, Won Do H. Methods Mol Biol. 2022;2446:595-606. doi: 10.1007/978-1-0716-2075-5_31. PubMed
A Nanobody-Based Toolset to Monitor and Modify the Mitochondrial GTPase Miro1. Fagbadebo FO, Kaiser PD, Zittlau K, Bartlick N, Wagner TR, Froehlich T, Jarjour G, Nueske S, Scholz A, Traenkle B, Macek B, Rothbauer U. Front Mol Biosci. 2022 Mar 10;9:835302. doi: 10.3389/fmolb.2022.835302. eCollection 2022. PubMed
Single-domain near-infrared protein provides a scaffold for antigen-dependent fluorescent nanobodies. Oliinyk OS, Baloban M, Clark CL, Carey E, Pletnev S, Nimmerjahn A, Verkhusha VV. Nat Methods. 2022 May 23. pii: 10.1038/s41592-022-01467-6. doi: 10.1038/s41592-022-01467-6. PubMed

Associated Plasmids

An AMPK phosphoregulated RhoGEF feedback loop tunes cortical flow-driven amoeboid migration in vivo. Lin B, Luo J, Lehmann R. Sci Adv. 2022 Sep 16;8(37):eabo0323. doi: 10.1126/sciadv.abo0323. Epub 2022 Sep 14. PubMed
gamma-2 and GSG1L bind with comparable affinities to the tetrameric GluA1 core. Yu C, Runge HFP, Mukhopadhyay A, Zolles G, Ulbrich MH. Cell Mol Biol Lett. 2023 Jul 10;28(1):54. doi: 10.1186/s11658-023-00470-9. PubMed
A TRIM21-based bioPROTAC highlights the therapeutic benefit of HuR degradation. Fletcher A, Clift D, de Vries E, Martinez Cuesta S, Malcolm T, Meghini F, Chaerkady R, Wang J, Chiang A, Weng SHS, Tart J, Wong E, Donohoe G, Rawlins P, Gordon E, Taylor JD, James L, Hunt J. Nat Commun. 2023 Nov 4;14(1):7093. doi: 10.1038/s41467-023-42546-2. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.