Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pcDNA3_NSlmb-vhhGFP4 Citations (10)

Plasmid Article: Fluorescent fusion protein knockout mediated by anti-GFP nanobody.
Caussinus E, Kanca O, Affolter M Nat Struct Mol Biol. 2011 Dec 11;19(1):117-21. doi: 10.1038/nsmb.2180.
PubMed Journal

Articles Citing pcDNA3_NSlmb-vhhGFP4

Articles
Dynamics of CDKN1A in Single Cells Defined by an Endogenous Fluorescent Tagging Toolkit. Stewart-Ornstein J, Lahav G. Cell Rep. 2016 Feb 10. pii: S2211-1247(16)30023-7. doi: 10.1016/j.celrep.2016.01.045. PubMed
Protein Inactivation by Optogenetic Trapping in Living Cells. Park H, Lee S, Heo WD. Methods Mol Biol. 2016;1408:363-76. doi: 10.1007/978-1-4939-3512-3_25. PubMed
A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans. Wang S, Tang NH, Lara-Gonzalez P, Zhao Z, Cheerambathur DK, Prevo B, Chisholm AD, Desai A, Oegema K. Development. 2017 Jun 15. pii: dev.150094. doi: 10.1242/dev.150094. PubMed

Associated Plasmids

A Method for the Acute and Rapid Degradation of Endogenous Proteins. Clift D, McEwan WA, Labzin LI, Konieczny V, Mogessie B, James LC, Schuh M. Cell. 2017 Nov 13. pii: S0092-8674(17)31255-2. doi: 10.1016/j.cell.2017.10.033. PubMed

Associated Plasmids

Sculpting ion channel functional expression with engineered ubiquitin ligases. Kanner SA, Morgenstern T, Colecraft HM. Elife. 2017 Dec 19;6. pii: 29744. doi: 10.7554/eLife.29744. PubMed
Conditional control of fluorescent protein degradation by an auxin-dependent nanobody. Daniel K, Icha J, Horenburg C, Muller D, Norden C, Mansfeld J. Nat Commun. 2018 Aug 17;9(1):3297. doi: 10.1038/s41467-018-05855-5. PubMed

Associated Plasmids

zGrad is a nanobody-based degron system that inactivates proteins in zebrafish. Yamaguchi N, Colak-Champollion T, Knaut H. Elife. 2019 Feb 8;8. pii: 43125. doi: 10.7554/eLife.43125. PubMed
Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins. Yu D, Lee H, Hong J, Jung H, Jo Y, Oh BH, Park BO, Heo WD. Nat Methods. 2019 Nov;16(11):1095-1100. doi: 10.1038/s41592-019-0592-7. Epub 2019 Oct 14. PubMed
Optogenetic regulation of endogenous proteins. Redchuk TA, Karasev MM, Verkhusha PV, Donnelly SK, Hulsemann M, Virtanen J, Moore HM, Vartiainen MK, Hodgson L, Verkhusha VV. Nat Commun. 2020 Jan 30;11(1):605. doi: 10.1038/s41467-020-14460-4. PubMed

Associated Plasmids

Collectively stabilizing and orienting posterior migratory forces disperses cell clusters in vivo. Lin B, Luo J, Lehmann R. Nat Commun. 2020 Sep 8;11(1):4477. doi: 10.1038/s41467-020-18185-2. PubMed

If you have published an article using this plasmid, please email us at [email protected] to have your article added to this page.