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(Plasmid #89368)

Full plasmid sequence is not available for this item.


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89368 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
    Christian Frøkjær-Jensen (Erik Jorgensen lab)
  • Backbone size w/o insert (bp) 7328
  • Total vector size (bp) 11594
  • Vector type
    Targeting vector for Mos1 transposon mediated single copy transgene insertion
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    C. elegans (nematode), Synthetic
  • Insert Size (bp)
  • Entrez Gene
    zif-1 (a.k.a. CELE_F59B2.6)
  • Promoter Posm-6
  • Tag / Fusion Protein
    • vhhGFP4 (GFP nanobody) (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tgtaaaacgacggccagt
  • 3′ sequencing primer CGACTCACTAGTGGGCAGAT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOD2048-csnDEG was a gift from Karen Oegema (Addgene plasmid # 89368 ; ; RRID:Addgene_89368)
  • For your References section:

    A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans. Wang S, Tang NH, Lara-Gonzalez P, Zhao Z, Cheerambathur DK, Prevo B, Chisholm AD, Desai A, Oegema K. Development. 2017 Jun 15. pii: dev.150094. doi: 10.1242/dev.150094. 10.1242/dev.150094 PubMed 28619826