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pOD2087-trnDEG
(Plasmid #89369)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89369 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCFJ151
  • Backbone manufacturer
    Christian Frøkjær-Jensen (Erik Jorgensen lab)
  • Backbone size w/o insert (bp) 7328
  • Total vector size (bp) 11150
  • Vector type
    Targeting vector for Mos1 transposon mediated single copy transgene insertion
  • Selectable markers
    Cb-unc-119(+)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    vhhGFP4-ZIF-1
  • Species
    C. elegans (nematode), Synthetic
  • Insert Size (bp)
    2098
  • Entrez Gene
    zif-1 (a.k.a. CELE_F59B2.6)
  • Promoter Pmec-18
  • Tag / Fusion Protein
    • vhhGFP4 (GFP nanobody) (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tgtaaaacgacggccagt
  • 3′ sequencing primer CGACTCACTAGTGGGCAGAT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOD2087-trnDEG was a gift from Karen Oegema (Addgene plasmid # 89369 ; http://n2t.net/addgene:89369 ; RRID:Addgene_89369)
  • For your References section:

    A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans. Wang S, Tang NH, Lara-Gonzalez P, Zhao Z, Cheerambathur DK, Prevo B, Chisholm AD, Desai A, Oegema K. Development. 2017 Jun 15. pii: dev.150094. doi: 10.1242/dev.150094. 10.1242/dev.150094 PubMed 28619826