Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Peft-3::cas9-SV40_NLS::tbb-2 3'UTR Citations (40)

Originally described in: Heritable genome editing in C. elegans via a CRISPR-Cas9 system.
Friedland AE, Tzur YB, Esvelt KM, Colaiacovo MP, Church GM, Calarco JA Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532.
PubMed Journal

Articles Citing Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

Articles
Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design. Farboud B, Meyer BJ. Genetics. 2015 Apr;199(4):959-71. doi: 10.1534/genetics.115.175166. Epub 2015 Feb 18. PubMed
Characterization of microsporidia-induced developmental arrest and a transmembrane leucine-rich repeat protein in Caenorhabditis elegans. Luallen RJ, Bakowski MA, Troemel ER. PLoS One. 2015 Apr 13;10(4):e0124065. doi: 10.1371/journal.pone.0124065. eCollection 2015. PubMed
Polymerase Theta is a key driver of genome evolution and of CRISPR/Cas9-mediated mutagenesis. van Schendel R, Roerink SF, Portegijs V, van den Heuvel S, Tijsterman M. Nat Commun. 2015 Jun 16;6:7394. doi: 10.1038/ncomms8394. PubMed
REC-1 and HIM-5 distribute meiotic crossovers and function redundantly in meiotic double-strand break formation in Caenorhabditis elegans. Chung G, Rose AM, Petalcorin MI, Martin JS, Kessler Z, Sanchez-Pulido L, Ponting CP, Yanowitz JL, Boulton SJ. Genes Dev. 2015 Sep 15;29(18):1969-79. doi: 10.1101/gad.266056.115. PubMed
Creating Genome Modifications in C. elegans Using the CRISPR/Cas9 System. Calarco JA, Friedland AE. Methods Mol Biol. 2015;1327:59-74. doi: 10.1007/978-1-4939-2842-2_6. PubMed
TMEM107 recruits ciliopathy proteins to subdomains of the ciliary transition zone and causes Joubert syndrome. Lambacher NJ, Bruel AL, van Dam TJ, Szymanska K, Slaats GG, Kuhns S, McManus GJ, Kennedy JE, Gaff K, Wu KM, van der Lee R, Burglen L, Doummar D, Riviere JB, Faivre L, Attie-Bitach T, Saunier S, Curd A, Peckham M, Giles RH, Johnson CA, Huynen MA, Thauvin-Robinet C, Blacque OE. Nat Cell Biol. 2016 Jan;18(1):122-31. doi: 10.1038/ncb3273. Epub 2015 Nov 23. PubMed
SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans. Schwartz ML, Jorgensen EM. Genetics. 2016 Feb 2. pii: genetics.115.184275. PubMed

Associated Plasmids

CRISPR-Cas9-Guided Genome Engineering in C. elegans. Kim HM, Colaiacovo MP. Curr Protoc Mol Biol. 2016 Jul 1;115:31.7.1-31.7.18. doi: 10.1002/cpmb.7. PubMed
A tissue-specific protein purification approach in Caenorhabditis elegans identifies novel interaction partners of DLG-1/Discs large. Waaijers S, Munoz J, Berends C, Ramalho JJ, Goerdayal SS, Low TY, Zoumaro-Djayoon AD, Hoffmann M, Koorman T, Tas RP, Harterink M, Seelk S, Kerver J, Hoogenraad CC, Bossinger O, Tursun B, van den Heuvel S, Heck AJ, Boxem M. BMC Biol. 2016 Aug 9;14:66. doi: 10.1186/s12915-016-0286-x. PubMed

Associated Plasmids

Multisite Phosphorylation of NuMA-Related LIN-5 Controls Mitotic Spindle Positioning in C. elegans. Portegijs V, Fielmich LE, Galli M, Schmidt R, Munoz J, van Mourik T, Akhmanova A, Heck AJ, Boxem M, van den Heuvel S. PLoS Genet. 2016 Oct 6;12(10):e1006291. doi: 10.1371/journal.pgen.1006291. eCollection 2016 Oct. PubMed
Removing bias against short sequences enables northern blotting to better complement RNA-seq for the study of small RNAs. Choi YS, Edwards LO, DiBello A, Jose AM. Nucleic Acids Res. 2017 Jun 2;45(10):e87. doi: 10.1093/nar/gkx091. PubMed
Two populations of cytoplasmic dynein contribute to spindle positioning in C. elegans embryos. Schmidt R, Fielmich LE, Grigoriev I, Katrukha EA, Akhmanova A, van den Heuvel S. J Cell Biol. 2017 Sep 4;216(9):2777-2793. doi: 10.1083/jcb.201607038. Epub 2017 Jul 24. PubMed
Targeted genome editing in Caenorhabditis elegans using CRISPR/Cas9. Farboud B. Wiley Interdiscip Rev Dev Biol. 2017 Nov;6(6). doi: 10.1002/wdev.287. Epub 2017 Aug 15. PubMed
Caenorhabditis elegans ATPase inhibitor factor 1 (IF1) MAI-2 preserves the mitochondrial membrane potential (Deltapsim) and is important to induce germ cell apoptosis. Fernandez-Cardenas LP, Villanueva-Chimal E, Salinas LS, Jose-Nunez C, Tuena de Gomez Puyou M, Navarro RE. PLoS One. 2017 Aug 22;12(8):e0181984. doi: 10.1371/journal.pone.0181984. eCollection 2017. PubMed
AIP limits neurotransmitter release by inhibiting calcium bursts from the ryanodine receptor. Chen B, Liu P, Hujber EJ, Li Y, Jorgensen EM, Wang ZW. Nat Commun. 2017 Nov 9;8(1):1380. doi: 10.1038/s41467-017-01704-z. PubMed
Tumor suppressor APC is an attenuator of spindle-pulling forces during C. elegans asymmetric cell division. Sugioka K, Fielmich LE, Mizumoto K, Bowerman B, van den Heuvel S, Kimura A, Sawa H. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):E954-E963. doi: 10.1073/pnas.1712052115. Epub 2018 Jan 18. PubMed
Transient and Partial Nuclear Lamina Disruption Promotes Chromosome Movement in Early Meiotic Prophase. Link J, Paouneskou D, Velkova M, Daryabeigi A, Laos T, Labella S, Barroso C, Pacheco Pinol S, Montoya A, Kramer H, Woglar A, Baudrimont A, Markert SM, Stigloher C, Martinez-Perez E, Dammermann A, Alsheimer M, Zetka M, Jantsch V. Dev Cell. 2018 Apr 23;45(2):212-225.e7. doi: 10.1016/j.devcel.2018.03.018. PubMed
Targeted Chromosomal Rearrangements via Combinatorial Use of CRISPR/Cas9 and Cre/LoxP Technologies in Caenorhabditis elegans. Chen X, Liao S, Huang X, Xu T, Feng X, Guang S. G3 (Bethesda). 2018 Jul 31;8(8):2697-2707. doi: 10.1534/g3.118.200473. PubMed
Distinct regions of the intrinsically disordered protein MUT-16 mediate assembly of a small RNA amplification complex and promote phase separation of Mutator foci. Uebel CJ, Anderson DC, Mandarino LM, Manage KI, Aynaszyan S, Phillips CM. PLoS Genet. 2018 Jul 23;14(7):e1007542. doi: 10.1371/journal.pgen.1007542. eCollection 2018 Jul. PubMed
Evolutionary plasticity in the innate immune function of Akirin. Polanowska J, Chen JX, Soule J, Omi S, Belougne J, Taffoni C, Pujol N, Selbach M, Zugasti O, Ewbank JJ. PLoS Genet. 2018 Jul 23;14(7):e1007494. doi: 10.1371/journal.pgen.1007494. eCollection 2018 Jul. PubMed
Rap2 and TNIK control Plexin-dependent tiled synaptic innervation in C. elegans. Chen X, Shibata AC, Hendi A, Kurashina M, Fortes E, Weilinger NL, MacVicar BA, Murakoshi H, Mizumoto K. Elife. 2018 Jul 31;7. pii: 38801. doi: 10.7554/eLife.38801. PubMed
FACT Sets a Barrier for Cell Fate Reprogramming in Caenorhabditis elegans and Human Cells. Kolundzic E, Ofenbauer A, Bulut SI, Uyar B, Baytek G, Sommermeier A, Seelk S, He M, Hirsekorn A, Vucicevic D, Akalin A, Diecke S, Lacadie SA, Tursun B. Dev Cell. 2018 Sep 10;46(5):611-626.e12. doi: 10.1016/j.devcel.2018.07.006. Epub 2018 Aug 2. PubMed
Optogenetic dissection of mitotic spindle positioning in vivo. Fielmich LE, Schmidt R, Dickinson DJ, Goldstein B, Akhmanova A, van den Heuvel S. Elife. 2018 Aug 15;7. pii: 38198. doi: 10.7554/eLife.38198. PubMed
SLO potassium channels antagonize premature decision making in C. elegans. Aoki I, Tateyama M, Shimomura T, Ihara K, Kubo Y, Nakano S, Mori I. Commun Biol. 2018 Aug 24;1:123. doi: 10.1038/s42003-018-0124-5. eCollection 2018. PubMed
EFHC1, implicated in juvenile myoclonic epilepsy, functions at the cilium and synapse to modulate dopamine signaling. Loucks CM, Park K, Walker DS, McEwan AH, Timbers TA, Ardiel EL, Grundy LJ, Li C, Johnson JL, Kennedy J, Blacque OE, Schafer W, Rankin CH, Leroux MR. Elife. 2019 Feb 27;8. pii: 37271. doi: 10.7554/eLife.37271. PubMed
A CCRK and a MAK Kinase Modulate Cilia Branching and Length via Regulation of Axonemal Microtubule Dynamics in Caenorhabditis elegans. Maurya AK, Rogers T, Sengupta P. Curr Biol. 2019 Apr 22;29(8):1286-1300.e4. doi: 10.1016/j.cub.2019.02.062. Epub 2019 Apr 4. PubMed
Differential Requirements for Centrioles in Mitotic Centrosome Growth and Maintenance. Cabral G, Laos T, Dumont J, Dammermann A. Dev Cell. 2019 Aug 5;50(3):355-366.e6. doi: 10.1016/j.devcel.2019.06.004. Epub 2019 Jul 11. PubMed
C. elegans Runx/CBFbeta suppresses POP-1 TCF to convert asymmetric to proliferative division of stem cell-like seam cells. van der Horst SEM, Cravo J, Woollard A, Teapal J, van den Heuvel S. Development. 2019 Nov 18;146(22). pii: dev.180034. doi: 10.1242/dev.180034. PubMed
Gradient-independent Wnt signaling instructs asymmetric neurite pruning in C. elegans. Lu M, Mizumoto K. Elife. 2019 Dec 5;8. pii: 50583. doi: 10.7554/eLife.50583. PubMed
A tudor domain protein, SIMR-1, promotes siRNA production at piRNA-targeted mRNAs in C. elegans. Manage KI, Rogers AK, Wallis DC, Uebel CJ, Anderson DC, Nguyen DAH, Arca K, Brown KC, Cordeiro Rodrigues RJ, de Albuquerque BF, Ketting RF, Montgomery TA, Phillips CM. Elife. 2020 Apr 27;9. pii: 56731. doi: 10.7554/eLife.56731. PubMed
Genome editing in the nematode Caenorhabditis briggsae using the CRISPR/Cas9 system. Culp E, Richman C, Sharanya D, Jhaveri N, van den Berg W, Gupta BP. Biol Methods Protoc. 2020 Feb 10;5(1):bpaa003. doi: 10.1093/biomethods/bpaa003. eCollection 2020. PubMed
Dose-dependent functions of SWI/SNF BAF in permitting and inhibiting cell proliferation in vivo. van der Vaart A, Godfrey M, Portegijs V, van den Heuvel S. Sci Adv. 2020 May 20;6(21):eaay3823. doi: 10.1126/sciadv.aay3823. eCollection 2020 May. PubMed
MCTP-1 modulates neurotransmitter release in C. elegans. Tellez-Arreola JL, Silva M, Martinez-Torres A. Mol Cell Neurosci. 2020 Jul 7;107:103528. doi: 10.1016/j.mcn.2020.103528. PubMed
Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules. Castiglioni VG, Pires HR, Rosas Bertolini R, Riga A, Kerver J, Boxem M. Elife. 2020 Dec 10;9. pii: 62067. doi: 10.7554/eLife.62067. PubMed
BBLN-1 is essential for intermediate filament organization and apical membrane morphology. Remmelzwaal S, Geisler F, Stucchi R, van der Horst S, Pasolli M, Kroll JR, Jarosinska OD, Akhmanova A, Richardson CA, Altelaar M, Leube RE, Ramalho JJ, Boxem M. Curr Biol. 2021 Jun 7;31(11):2334-2346.e9. doi: 10.1016/j.cub.2021.03.069. Epub 2021 Apr 14. PubMed
Arginine methylation promotes siRNA-binding specificity for a spermatogenesis-specific isoform of the Argonaute protein CSR-1. Nguyen DAH, Phillips CM. Nat Commun. 2021 Jul 9;12(1):4212. doi: 10.1038/s41467-021-24526-6. PubMed
Sustained expression of unc-4 homeobox gene and unc-37/Groucho in postmitotic neurons specifies the spatial organization of the cholinergic synapses in C. elegans. Kurashina M, Wang J, Lin J, Lee KK, Johal A, Mizumoto K. Elife. 2021 Aug 13;10. pii: 66011. doi: 10.7554/eLife.66011. PubMed
Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells. Riga A, Cravo J, Schmidt R, Pires HR, Castiglioni VG, van den Heuvel S, Boxem M. PLoS Genet. 2021 Oct 21;17(10):e1009856. doi: 10.1371/journal.pgen.1009856. eCollection 2021 Oct. PubMed
ERM-1 Phosphorylation and NRFL-1 Redundantly Control Lumen Formation in the C. elegans Intestine. Sepers JJ, Ramalho JJ, Kroll JR, Schmidt R, Boxem M. Front Cell Dev Biol. 2022 Feb 7;10:769862. doi: 10.3389/fcell.2022.769862. eCollection 2022. PubMed
Targeting endogenous proteins for spatial and temporal knockdown using auxin-inducible degron in Caenorhabditis elegans. Kurashina M, Mizumoto K. STAR Protoc. 2023 Jan 13;4(1):102028. doi: 10.1016/j.xpro.2022.102028. PubMed

Associated Plasmids

If you have published an article using this material, please email us at [email protected] to have your article added to this page.