165,688 results

1. pDD112

   Plasmid

   #119893

   Purpose

   pNG2 backbone with an optimized chloramphenicol resitance marker for Rhodococcus opacus PD630

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ubc.Luc.IRES.Puro

   Plasmid

   #33307

   Depositor

   Linzhao Cheng

   Article

   Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   Ubc

   Available Since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUBC-SV40NLS-Pfv-Sapphire-PURO

   Plasmid

   #203654

   Purpose

   Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Lentiviral

   Tags

   Sapphire

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBtet-GH

   Plasmid

   #60498

   Purpose

   SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and hygromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   TCE & RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

   Plasmid

   #179105

   Purpose

   pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRL-TK CXCR4 2x

   Plasmid

   #11306

   Depositor

   Phil Sharp

   Article

   Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

   Insert

   2 bulged bind sites for CXCR4 siRNA antisense
   

   Use

   Luciferase

   Tags

   Rr-luc

   Available Since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMini-CMV-NLS-dead R-IscB (D60A, H269A)-NLS_T2A_mCherry_U6-ωRNA

   Plasmid

   #246430

   Purpose

   All-in-one plasmid. Expresses R-IscB in mammalian cells. Dead mutations included.

   Depositor

   Ailong Ke

   Article

   Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

   Inserts

   O.gue IscB with dead mutations (D60A, H269A) and delta-TID
   mCherry
   ωRNA
   

   Tags

   SV40 NLS, Thosea asigna virus 2A peptide, and nuc…

   Expression

   Mammalian

   Mutation

   changed Aspartic Acid 60 to Alanine, changed Hist…

   Promoter

   CMV and U6

   Available Since

   Oct. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry- SEC61B

   Plasmid

   #121160

   Purpose

   Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   SEC61B (SEC61B )
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

   Plasmid

   #134867

   Purpose

   Expresses EGFP-tagged nuclear envelope marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Lamin A (LMNA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE2-TetOminiCMV-SV40LT

    Plasmid

    #136616

    Purpose

    Dox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cells

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    SV40 large T antigen
    

    Use

    Lentiviral

    Expression

    Bacterial

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmiRFP670-N1

    Plasmid

    #79987

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670 in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Has Service

    Cloning Grade DNA

    Insert

    miRFP670
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid

    #140003

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tet-on circRNA Expression Vector

    Plasmid

    #92351

    Purpose

    The vector can ef_ciently produce a circRNA (circmCherry) from a split EGFP gene after Dox treatment.

    Depositor

    Ling-Ling Chen

    Article

    Zhang et al Cell Rep. 2016 Apr 19;15(3):611-24. doi:

    Insert

    mCherry and split EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    pTet

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-mCherry-CNA35

    Plasmid

    #61607

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with mCherry

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mCherry and CNA35
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLv-HSA-uDys/eGFP

    Plasmid

    #26810

    Depositor

    Jeffrey Chamberlain

    Article

    Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

    Insert

    dystrophin (DMD Human)
    

    Use

    Lentiviral; Lentiviral plasmid backbone from l. n…

    Tags

    eGFP

    Mutation

    deleted R4-R23 deleted exons 71-78

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMT525

    Plasmid

    #131392

    Purpose

    L1RP retrotransposition reporter

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    L1RP GFP-AI
    

    Expression

    Mammalian

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHSN6I01

    Plasmid

    #50587

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-KRAB
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Mutation

    dCas9 that is defective in DNA cleavage; the maiz…

    Promoter

    2×35Sp and AtU6-26p

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCI-His-hUbi

    Plasmid

    #31815

    Depositor

    Astar Winoto

    Article

    Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31.

    Insert

    Ubiquitin (UBB Human)
    

    Tags

    HIS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRXIP TMEM41B-3xFLAG

    Plasmid

    #117415

    Purpose

    Expresses TMEM41B tagged with 3xFLAG in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    TMEM41B (TMEM41B Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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