163,584 results

1. pAcGFP1-N1-Dsup-C

   Plasmid

   #90022

   Purpose

   To express GFP-tagged C-terminal region of Dsup in mammalian cells transiently

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   C-terminal region alone

   Promoter

   CMV

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFLAG_attP

   Plasmid

   #110095

   Purpose

   Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBP-J23100

   Plasmid

   #72963

   Purpose

   Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   J23100 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCer-Mcm5

   Plasmid

   #211391

   Purpose

   mammalian expression of human Mcm5 N-terminally fused to mCerulean

   Depositor

   Helmut Pospiech

   Insert

   Mcm5 (MCM5 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPGK-T7/2-CD44v8-10 (fl)

   Plasmid

   #137823

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v8-10 (fl) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   full length

   Promoter

   PGK

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS2705-CMV-Nme2Smu-ABE8e-i8-L10-D56R

   Plasmid

   #228726

   Purpose

   Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-D56R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A D56R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS2704-CMV-Nme2Smu-ABE8e-i8-L10-E932R

   Plasmid

   #228725

   Purpose

   Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-E932R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEJS2708-CMV-Nme2Smu-ABE8e-i1-L10-E932R

   Plasmid

   #228729

   Purpose

   Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-E932R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEJS2706-CMV-Nme2-ABE8e-i8-L10-E932R

   Plasmid

   #228727

   Purpose

   Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2 nCas9 (D16A)-i8-L10-E932R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEJS2707-CMV-Nme2-ABE8e-i8-L10-D56R

    Plasmid

    #228728

    Purpose

    Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2 nCas9 (D16A)-i8-L10-D56R
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A D56R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEJS2709-CMV-Nme2Smu-ABE8e-i1-L10-D56R

    Plasmid

    #228730

    Purpose

    Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-D56R
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D16A D56R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEJS2712-CMV-eNme2-C-ABE8e-i1-L10

    Plasmid

    #228733

    Purpose

    Expresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid eNme-C nCas9 (D16A)-i1-L10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Stx5S-mCherry

    Plasmid

    #165087

    Purpose

    Expresses Stx5S-mCherry in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    Stx5 (Short isoform) (STX5 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Lacks amino acids 1-54, enocdes short isoform of …

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAB107a

    Plasmid

    #79218

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    May 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-Tnos

    Plasmid

    #211845

    Purpose

    Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Inserts

    LRR-NEL
    GAL4-VP16-GR
    

    Use

    Synthetic Biology

    Tags

    HA, Myc, and mCherry

    Expression

    Plant

    Mutation

    C492A

    Promoter

    6xUAS-35Smini and pUBQ10

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF1684

    Plasmid

    #143965

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HNF1B (HNF1B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. H_NES 41kDa

    Plasmid

    #201346

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    HIV_NES-EGFP-2PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. UAS-Syt::7xHalo7

    Plasmid

    #87650

    Purpose

    For creating drosophila transgenics expressing UAS-Syt::7xHalo7

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    Syt::7xHalo7
    

    Use

    Tags

    Expression

    Insect

    Mutation

    S265G in Syt (please see depositor's commen…

    Promoter

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLXI_TRC401 SMARCB1 var 2

    Plasmid

    #111182

    Purpose

    Inducible vector (untagged) with SMARCB1 variant 2

    Depositor

    William Hahn

    Article

    Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

    Insert

    BAF47 (SMARCB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBlueBac human APC2

    Plasmid

    #11425

    Depositor

    Marc Kirschner

    Article

    Yu et al Science. 1998 Feb 20. 279(5354):1219-22.

    Insert

    APC2 (ANAPC2 Human)
    

    Use

    Baculoviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only