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pcDNA3-SARS-CoV-2-S-RBD-8his Citations (13)

Originally described in: Engineering human ACE2 to optimize binding to the spike protein of SARS coronavirus 2.
Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert AS, Procko E Science. 2020 Sep 4;369(6508):1261-1265. doi: 10.1126/science.abc0870. Epub 2020 Aug 4.
PubMed Journal

Articles Citing pcDNA3-SARS-CoV-2-S-RBD-8his

Articles
The sequence of human ACE2 is suboptimal for binding the S spike protein of SARS coronavirus 2. Procko E. bioRxiv. 2020 May 11. doi: 10.1101/2020.03.16.994236. PubMed
Heterogeneous antibodies against SARS-CoV-2 spike receptor binding domain and nucleocapsid with implications for COVID-19 immunity. McAndrews KM, Dowlatshahi DP, Dai J, Becker LM, Hensel J, Snowden LM, Leveille JM, Brunner MR, Holden KW, Hopkins NS, Harris AM, Kumpati J, Whitt MA, Lee JJ, Ostrosky-Zeichner LL, Papanna R, LeBleu VS, Allison JP, Kalluri R. JCI Insight. 2020 Sep 17;5(18). pii: 142386. doi: 10.1172/jci.insight.142386. PubMed
An engineered decoy receptor for SARS-CoV-2 broadly binds protein S sequence variants. Chan KK, Tan TJC, Narayanan KK, Procko E. Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf1738. doi: 10.1126/sciadv.abf1738. Print 2021 Feb. PubMed

Associated Plasmids

Structures of synthetic nanobody-SARS-CoV-2-RBD complexes reveal distinct sites of interaction and recognition of variants. Margulies D, Ahmad J, Jiang J, Boyd L, Zeher A, Huang R, Xia D, Natarajan K. Res Sq. 2021 Jun 16. doi: 10.21203/rs.3.rs-625642/v1. PubMed
Computationally Designed ACE2 Decoy Receptor Binds SARS-CoV-2 Spike (S) Protein with Tight Nanomolar Affinity. Havranek B, Chan KK, Wu A, Procko E, Islam SM. J Chem Inf Model. 2021 Sep 27;61(9):4656-4669. doi: 10.1021/acs.jcim.1c00783. Epub 2021 Aug 24. PubMed
Structures of synthetic nanobody-SARS-CoV-2 receptor-binding domain complexes reveal distinct sites of interaction. Ahmad J, Jiang J, Boyd LF, Zeher A, Huang R, Xia D, Natarajan K, Margulies DH. J Biol Chem. 2021 Oct;297(4):101202. doi: 10.1016/j.jbc.2021.101202. Epub 2021 Sep 16. PubMed
Sustained Delivery of SARS-CoV-2 RBD Subunit Vaccine Using a High Affinity Injectable Hydrogel Scaffold. Chen J, Wang B, Caserto JS, Shariati K, Cao P, Pan Y, Xu Q, Ma M. Adv Healthc Mater. 2022 Jan;11(2):e2101714. doi: 10.1002/adhm.202101714. Epub 2021 Nov 16. PubMed
A Mycobacteriophage-Based Vaccine Platform: SARS-CoV-2 Antigen Expression and Display. Freeman KG, Wetzel KS, Zhang Y, Zack KM, Jacobs-Sera D, Walters SM, Barbeau DJ, McElroy AK, Williams JV, Hatfull GF. Microorganisms. 2021 Nov 23;9(12). pii: microorganisms9122414. doi: 10.3390/microorganisms9122414. PubMed
An engineered ACE2 decoy receptor can be administered by inhalation and potently targets the BA.1 and BA.2 omicron variants of SARS-CoV-2. Zhang L, Narayanan KK, Cooper L, Chan KK, Devlin CA, Aguhob A, Shirley K, Rong L, Rehman J, Malik AB, Procko E. bioRxiv. 2022 Mar 28. doi: 10.1101/2022.03.28.486075. PubMed
A rapid bead-based assay for screening of SARS-CoV-2 neutralizing antibodies. Lupitha SS, Darvin P, Chandrasekharan A, Varadarajan SN, Divakaran SJ, Easwaran S, Nelson-Sathi S, Umasankar PK, Jones S, Joseph I, Pillai MR, Santhoshkumar TR. Antib Ther. 2022 Mar 17;5(2):100-110. doi: 10.1093/abt/tbac007. eCollection 2022 Apr. PubMed
Maximization of the Minicircle DNA Vaccine Production Expressing SARS-CoV-2 RBD. Ventura C, Eusebio D, Goncalves AM, Barroca-Ferreira J, Costa D, Cui Z, Passarinha LA, Sousa A. Biomedicines. 2022 Apr 25;10(5):990. doi: 10.3390/biomedicines10050990. PubMed
Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination. Fertig TE, Chitoiu L, Marta DS, Ionescu VS, Cismasiu VB, Radu E, Angheluta G, Dobre M, Serbanescu A, Hinescu ME, Gherghiceanu M. Biomedicines. 2022 Jun 28;10(7):1538. doi: 10.3390/biomedicines10071538. PubMed
Efficient Purification of Polyhistidine-Tagged Recombinant Proteins Using Functionalized Corundum Particles. Volzke JL, Smatty S, Doring S, Ewald S, Oelze M, Fratzke F, Flemig S, Konthur Z, Weller MG. BioTech (Basel). 2023 May 3;12(2):31. doi: 10.3390/biotech12020031. PubMed

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