163,486 results

1. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138150

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR1_BsmBI
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   DR1 A>T

   Promoter

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-DDX3X

   Plasmid

   #116730

   Purpose

   Lentiviral expression of DDX3X

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   DDX3X (DDX3X Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57.1 CMYC

   Plasmid

   #164145

   Purpose

   Doxycycline inducible expression of c-MYC

   Depositor

   Richard Possemato

   Insert

   MYC (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Doxycycline inducible promoter

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. dCas9-KRAB

   Plasmid

   #110820

   Purpose

   Conventional dCas9 repressor-dCas9-KRAB

   Depositor

   George Church, Alejandro Chavez

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ef1a_Large T-antigen_Ires_Puro

   Plasmid

   #18922

   Depositor

   Linzhao Cheng

   Article

   Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

   Insert

   Large T Antigen (SV40gp6 Simian virus 40)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. FU-tetO-Gateway

   Plasmid

   #43914

   Depositor

   John Gearhart

   Article

   Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. rtTA-N144

   Plasmid

   #66810

   Purpose

   2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance

   Depositor

   Andrew Yoo

   Article

   Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

   Insert

   Reverse tetracycline-controlled transactivator
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a

   Available Since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBR322-PVCpnf17-22

   Plasmid

   #198272

   Purpose

   Unmodified pPayload; contains WT PVC payloads/regulatory genes

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf17-22
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-SFFV-FLAG-SQSTM1

   Plasmid

   #223715

   Purpose

   Expresses Flag-tagged SQSTM1

   Depositor

   Benjamin Wolozin

   Article

   Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

   Insert

   SQSTM1 (SQSTM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. CMV-Mito4x-GCaMP6f

    Plasmid

    #127870

    Purpose

    Fluorescent reporter for mitochondrial matrix Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    4 repeats of COX8 signal peptide plus GCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR_SFFV

    Plasmid

    #79121

    Purpose

    Lentiviral vector for constitutive expression of genes (SFFV promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX_lenti_osTir1_9Myc_P2A_Bsd

    Plasmid

    #139746

    Purpose

    lentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection marker

    Depositor

    Robert Tjian

    Article

    Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

    Insert

    rice E3 ligase Tir1 (osTir1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 MDM2 WT

    Plasmid

    #16233

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA-HIF2alpha-P405A/P531A-pcDNA3

    Plasmid

    #18956

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-U6/sgClta-sfGFP-Clta

    Plasmid

    #200261

    Purpose

    U6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

    Depositor

    Justin Eyquem

    Article

    Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

    Insert

    sfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-CD14

    Plasmid

    #13645

    Depositor

    Doug Golenbock

    Insert

    CD14 (CD14 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. 4xNFkB Luc

    Plasmid

    #111216

    Purpose

    reporter gene

    Depositor

    Johannes A. Schmid

    Insert

    firefly Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPSU150

    Plasmid

    #173836

    Purpose

    Expresses 150 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABMBPpepN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLEX_307

    Plasmid

    #41392

    Purpose

    Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lact-C2-GFP

    Plasmid

    #22852

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

    Insert

    Lactadherin-C2 Domain (MFGE8 Bovine)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge