165,655 results

1. pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST

   Plasmid

   #49771

   Purpose

   Level 1 hCas9 module

   Depositor

   Sophien Kamoun

   Article

   Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

   Insert

   35Sp::hCas9
   

   Use

   CRISPR

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRosetta_v2

   Plasmid

   #136477

   Purpose

   Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
   

   Use

   Lentiviral

   Promoter

   PGK

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC.HA Vav2

   Plasmid

   #14554

   Depositor

   Joan Brugge

   Article

   Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

   Insert

   Vav2 (VAV2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-IRES-YFP II (pMIY II)

   Plasmid

   #52108

   Purpose

   a bicistronic IRES-YFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-EYFP

   Expression

   Mammalian

   Available Since

   March 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. 12xMoonTag-12xSunTag-kif18b-24xPP7

   Plasmid

   #128606

   Purpose

   Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Inserts

   12xMoonTag-12xSunTag
   kinesin family member 18b (KIF18B Human)
   

   Expression

   Mammalian

   Promoter

   Pcmv

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-DIO-GRAB_g5-HT3.0

   Plasmid

   #208726

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinase

   Depositor

   Yulong Li

   Article

   Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmNeonGreenHO-G

   Plasmid

   #127912

   Purpose

   a mammalian expression backbone vector for humanized mNeonGreen

   Depositor

   Isei Tanida

   Article

   Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

   Insert

   humanized mNeonGreen
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP/golgin-160

   Plasmid

   #180630

   Purpose

   Expression of WT golgin-160

   Depositor

   Carolyn Machamer

   Article

   Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

   Insert

   golgin-160 (GOLGA3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-As-crRNA

   Plasmid

   #78956

   Purpose

   Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-EGFP-AID-BubR1

    Plasmid

    #47330

    Purpose

    for plasmid integration using the Flp-In system. AID at N-terminal

    Depositor

    Don Cleveland

    Article

    Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

    Insert

    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    

    Tags

    AID and GFP

    Expression

    Mammalian

    Mutation

    siRNA resistant

    Available Since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUGW-Golgi-TurboID

    Plasmid

    #200964

    Purpose

    3rd gen lentiviral plasmid for expressing golgi localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Golgi-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLPT41

    Plasmid

    #85524

    Purpose

    Titration sponge for TetR

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Promoter

    PLtet

    Available Since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40

    Plasmid

    #100054

    Purpose

    AAV expression of humanized ChR2 with H134R mutation fused to mCherry driven by CAG promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV1, AAV2, AAV5, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 Myc2- Hs TTP WT

    Plasmid

    #107008

    Purpose

    Expresses epitope-tagged wild-type human TTP

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    tristetraprolin (ZFP36 Human)
    

    Tags

    Double Myc peptide epitope tag

    Expression

    Mammalian

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Casp2-Flag

    Plasmid

    #11811

    Depositor

    Guy Salvesen

    Insert

    Caspase-2 (CASP2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC57 (Gent)

    Plasmid

    #54338

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV_Zika_NS5_Flag

    Plasmid

    #79639

    Purpose

    Third generation Self-inactivating lentivector expressing NS5 from Zika virus

    Depositor

    Vaithi Arumugaswami

    Insert

    NS5
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTipi2.1-AZU_SP

    Plasmid

    #229144

    Purpose

    Allows for expression of recombinant antibodies. Includes human azurocidin signal peptide.

    Depositor

    Institute for Protein Innovation (IPI)

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Jan. 9, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. GFP-Tollip

    Plasmid

    #178050

    Purpose

    GFP fused to human Tollip cDNA. Localisation and expression in mammalian cells.

    Depositor

    David Tumbarello

    Article

    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

    Insert

    Toll-Interacting Protein (TOLLIP Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

    Plasmid

    #101209

    Purpose

    Bacterial expression plasmid for SpCas9-HF1 variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N497A/R661A/Q695A/Q926A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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