166,806 results

1. pCRIS-PITChv2-Puro-dTAG (BRD4)

   Plasmid

   #91793

   Purpose

   PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   Puro-P2A-2xHA-FKBP_F36V
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promotorless

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRC0569 (parts entry vector)

   Plasmid

   #189966

   Purpose

   Cloning vector for generating part plasmids, with mNeonGreen dropout following BsmBI digestion

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Expression

   Mammalian

   Promoter

   BBa-J23100

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. EC67842

   Plasmid

   #209516

   Purpose

   Cloning in complete guide expression cassettes, with Cas12a (D156R)

   Depositor

   Wendy Harwood, Cristobal Uauy

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVS1-tet-iCas9-BFP2

   Plasmid

   #125519

   Purpose

   all-in-one-inducible Cas9

   Depositor

   Jason Moffat

   Article

   Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

   Insert

   AAVS1-TET-ON 3G-SpCas9-P2A-BFP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCherry Paxillin

   Plasmid

   #50526

   Depositor

   Kenneth Yamada

   Insert

   PXN paxillin (PXN Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 APEX2-NES

   Plasmid

   #49386

   Purpose

   Expresses APEX2 in the cytosol of mammalian cells

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   Flag - APEX2 - NES
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K14D, W41F, E112K, A134P on soybean APX; improved…

   Promoter

   CMV

   Available Since

   Nov. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1+/-FLI1-C‐(K)‐DYK

   Plasmid

   #129497

   Purpose

   Mammalian expression of human FLI1

   Depositor

   Marjorie Brand

   Article

   Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

   Insert

   FLI1 (FLI1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL-MS2-12X

   Plasmid

   #27119

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   MS2 binding sites (12)
   

   Expression

   Bacterial

   Available Since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTn Donor

   Plasmid

   #236211

   Purpose

   Arabinose-inducible Tn5 InducTn-seq plasmid

   Depositor

   Matthew Waldor

   Article

   Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

   Insert

   None
   

   Expression

   Bacterial

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIF1079

    Plasmid

    #237445

    Purpose

    Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.

    Depositor

    IIya Finkelstein

    Article

    Buffington et al bioRxiv 2024.07.21.604473; doi: 10.1101/

    Inserts

    SpCas9
    Efe1-RT
    EMX1 sgRNA
    Efe1-ncRNA targeting EMX1
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CAG, H1, and U6

    Available Since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-KRAS

    Plasmid

    #116755

    Purpose

    Lentiviral expression of KRAS

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    KRAS (KRAS Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-ExRai-AMPKAR(T/A)

    Plasmid

    #192447

    Purpose

    Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR(T/A)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Thr 6 in AMPK substrate mutated to Ala.

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTransposon.CAG.CasRX-P2A-Blast

    Plasmid

    #212965

    Purpose

    Episomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    CasRx
    

    Use

    Transposon

    Tags

    P2A-Blasticidin resistance

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. NLRP3-mNeonGreen

    Plasmid

    #200942

    Purpose

    NLRP3 fused with mNeonGreen to indicate its localization change

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV hSyn FLEx-loxp Kir2.1-2A-GFP

    Plasmid

    #161574

    Purpose

    Expression of Kir2.1-2A-GFP in a Cre-dependent fashion

    Depositor

    Kevin Beier, Robert Malenka

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    Kir2.1-2A-GFP (Kcnj2 Mouse)
    

    Use

    AAV

    Mutation

    NA

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. BiFC3

    Plasmid

    #190048

    Purpose

    BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 173–238)

    Depositor

    Patrick Van Dijck

    Article

    Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

    Insert

    YemVenus (ATG-aa173-238)
    

    Expression

    Yeast

    Promoter

    Met3

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. BiFC4

    Plasmid

    #190049

    Purpose

    BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 173–238)

    Depositor

    Patrick Van Dijck

    Article

    Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

    Insert

    YemVenus (ATG-aa173-238)
    

    Expression

    Yeast

    Promoter

    Met3

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. BiFC2

    Plasmid

    #190047

    Purpose

    BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 1–172)

    Depositor

    Patrick Van Dijck

    Article

    Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

    Insert

    YemVenus (aa1-172 - TAA)
    

    Expression

    Yeast

    Promoter

    Met3

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. BiFC1

    Plasmid

    #190046

    Purpose

    BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 1–172)

    Depositor

    Patrick Van Dijck

    Article

    Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

    Insert

    YemVenus (aa1-172)
    

    Expression

    Yeast

    Promoter

    Met3

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-R-GECO1.2

    Plasmid

    #45494

    Purpose

    Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    R-GECO1.2
    

    Expression

    Mammalian

    Mutation

    R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

    Promoter

    CMV

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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