162,717 results

1. p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

   Plasmid

   #224536

   Purpose

   A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   IRES-FLAG- NLS-mScarlet-I3
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSWAP_Entry_H2_beta_CM

   Plasmid

   #184870

   Purpose

   pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 2, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pHiUGE GS-gRNA (hSyn-Cre) backbone

   Plasmid

   #200368

   Purpose

   Backbone plasmid for cloning HiUGE GS-gRNA (with hSyn-Cre)

   Depositor

   Scott Soderling

   Article

   Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3xFLAG-CMV10-hUVRAG

   Plasmid

   #24292

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

   Insert

   UV irradiation resistance-associated gene (UVRAG Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pET-51b(+)_NAD-Snifit

   Plasmid

   #113658

   Purpose

   Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NAD-Snifit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWZL Neo Myr Flag DLG5

   Plasmid

   #20477

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   DLG5 (DLG5 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pHiUGE smFP-V5 donor ORF0

   Plasmid

   #200380

   Purpose

   Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

   Insert

   spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHiUGE smFP-V5 donor ORF1

   Plasmid

   #200381

   Purpose

   Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

   Insert

   spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTol1:uas-myc-act-map2k2b-2a-tdTomato

   Plasmid

   #104721

   Purpose

   UAS-driven expression of activated MEK

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   activated map2k2b (map2k2b Zebrafish)
   

   Use

   Zebrafish transposon

   Tags

   2A-tdTomato and myc tag

   Expression

   Mutation

   serines 221 and 225 to aspartic acid

   Promoter

   10xUAS

   Available since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1 mTagBFP2 rat PGK1 shRNA

    Plasmid

    #222869

    Purpose

    Expresses mTagBFP2 along with an shRNA against rat PGK1

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PGK1 shRNA (Pgk1, Pgk1 Mouse, Rat)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSWAP_Entry_H1_alpha

    Plasmid

    #184865

    Purpose

    pSwap plasmid part with H1_alpha homology arms

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pET-29b(+)_NSP4ss_SerM_NucA-myc

    Plasmid

    #218191

    Purpose

    Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Use

    Tags

    NSP4 signal secretion peptide and myc

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-HA-NLGN3 R451C

    Plasmid

    #59319

    Purpose

    Expression plasmid of HA-tagged human Neuroligin 3 harboring an R451C mutation

    Depositor

    Peter Scheiffele

    Article

    Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

    Insert

    NLGN3 R451C (NLGN3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Arginine 451 to Cysteine (R451C mutation)

    Promoter

    CAG

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-Spy-CD86

    Plasmid

    #223598

    Purpose

    Lentiviral expression of human CD86 with SpyTag003 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD86 (CD86 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    Spytag003

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-ARAF

    Plasmid

    #23725

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ARAF (ARAF Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pASKt15C+SrtA7

    Plasmid

    #64986

    Purpose

    Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, E105K, E108A, D16…

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCk2_spacer

    Plasmid

    #136072

    Purpose

    Use when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. CMV-mEGFP(A206K)-paCaMKII(T286A)

    Plasmid

    #165439

    Purpose

    Expresses photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(T286A)
    

    Use

    Tags

    mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    T286A: Autophosphorylation deficient mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti Vp64-dCas9 T2A mTagBFP2

    Plasmid

    #171052

    Purpose

    Expression of Vp64-dCas9 activation system

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    Vp64-dCas9 T2A mTagBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-51b(+)_NADP-Snifit

    Plasmid

    #113913

    Purpose

    Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge