166,806 results

1. pHAGE TRE dCas9-KRAB

   Plasmid

   #50917

   Purpose

   Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and KRAB

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   TRE

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MSCVneo-HA-ER-Hoxb8

   Plasmid

   #222291

   Purpose

   The most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.

   Depositor

   Mark Kamps, David Sykes

   Article

   Wang et al Nat Methods. 2006 Apr;3(4):287-93.

   Insert

   ER-Hoxb8 (ESR1, Hoxb8 Human, Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The estrogen receptor hormone binding domain cont…

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p3xnls-mScarlet3_N1

   Plasmid

   #189775

   Purpose

   Construct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   3xnls-mScarlet3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Hsp90 HA

   Plasmid

   #22487

   Depositor

   William Sessa

   Article

   Garcia-Cardena et al Nature. 1998 Apr 23. 392(6678):821-4.

   Insert

   Hsp90 HA (HSP90AB1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBOB-CAG-SARS-CoV2-Spike-HA

   Plasmid

   #141347

   Purpose

   3rd Generation lentiviral vector expressing the codon-optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

   Depositor

   Gerald Pao

   Article

   Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

   Insert

   SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   resynthesized with human codon optimization nucle…

   Promoter

   CAG

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. eeBxb1_IVT

   Plasmid

   #222342

   Purpose

   Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized evolved and engineered Bxb1
   

   Use

   Template for in vitro transcription

   Promoter

   T7 (inactivated)

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2+HyPer7-NLS

   Plasmid

   #136468

   Purpose

   Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Tags

   Triple nuclear localization signal of SV40 (simia…

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmGFP-P2A-K0-P2A-RFP

   Plasmid

   #105686

   Purpose

   Double fluorescent stalling reporter K0

   Depositor

   Ramanujan Hegde

   Article

   Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

   Inserts

   P2A-3xFLAG-VHPbeta
   K0
   P2A-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV (from vector)

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mTFP1-N1

   Plasmid

   #54521

   Purpose

   Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

   Depositor

   Robert Campbell, Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Type

   Empty backbone

   Tags

   mTFP1

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lifeact-3xmNG::LEU2

    Plasmid

    #227594

    Purpose

    Integration vector for expressing Lifeact-3xmNeonGreen

    Depositor

    Bruce Goode

    Article

    Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

    Insert

    Lifeact-3xmNeonGreen::LEU2
    

    Tags

    3xmNeonGreen

    Expression

    Yeast

    Mutation

    Truncated ABP140 (aa 1-17 only)

    Promoter

    Abp140

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGBKCg

    Plasmid

    #20162

    Depositor

    Peter Uetz

    Article

    Stellberger et al Proteome Sci. 2010 Feb 15;8:8. doi: 10.1

    Type

    Empty backbone

    Tags

    Gal4 DBD

    Expression

    Yeast

    Available Since

    May 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDonor_e-attP-H1-g3-Ef1a-Puro-P2A-mCherry

    Plasmid

    #247160

    Purpose

    Engineered e-attP donor plasmid expressing puromycin resistance and mCherry with donor binding sgRNA H1-g3

    Depositor

    Patrick Hsu

    Article

    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

    Insert

    Puromycin resistance, mCherry
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pXPR_050

    Plasmid

    #96925

    Purpose

    lentiviral expression of gRNA scaffold

    Depositor

    John Doench, David Root

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGFP-Cytochrome C

    Plasmid

    #41181

    Depositor

    Douglas Green

    Article

    Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

    Insert

    Cytochrome C (CYCS Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL4-AARE-luc2P-Hygro

    Plasmid

    #101787

    Purpose

    Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    3xAARE
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    minimal TATA-box promoter with low basal activity

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDOXOFF 99xGly

    Plasmid

    #211346

    Purpose

    DOX-regulated lentiviral expression of 99xGly-EGFP-3xHA

    Depositor

    Raghu Chivukula

    Article

    Yang et al Science. 2025 Jul 17;389(6757):eado2403.

    Insert

    99xGly-EGFP-3xHA
    

    Use

    Lentiviral

    Tags

    EGFP-3xHA

    Available Since

    Oct. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGADCg

    Plasmid

    #20161

    Depositor

    Peter Uetz

    Article

    Stellberger et al Proteome Sci. 2010 Feb 15;8:8. doi: 10.1

    Type

    Empty backbone

    Tags

    Gal4 AD and SV40 NLS

    Expression

    Yeast

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZR071_SFFV-dCas9-mCherry-KRAB

    Plasmid

    #180264

    Purpose

    Lentiviral expression vector for dCas9-KRAB in mammalian cells

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    KRAB and mCherry

    Expression

    Mammalian

    Mutation

    Nuclease dead Cas9

    Promoter

    SFFV

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKD4

    Plasmid

    #45605

    Depositor

    Barry L. Wanner

    Article

    Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

    Insert

    FRT-kan-FRT
    

    Expression

    Bacterial

    Promoter

    R6K

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ABE8e-SpRY

    Plasmid

    #226858

    Purpose

    Expresses ABE8e-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8e-SpRY
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge