165,214 results

1. pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

   Plasmid

   #188768

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Promoter

   SFFV

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. L4440

   Plasmid

   #1654

   Depositor

   Andrew Fire

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   RNAi

   Tags

   T7p, T7p, lacZN, OriF1>>, OriF1<<

   Expression

   Worm

   Available Since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. PCS2+Cas9-mSA

   Plasmid

   #103882

   Purpose

   Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   CAS9-MSA
   

   Use

   CRISPR

   Tags

   MSA (monomeric streptavidin

   Mutation

   none

   Promoter

   SP6

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIJ8

   Plasmid

   #68122

   Purpose

   Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

   Depositor

   Alex Nielsen

   Article

   Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

   Inserts

   flp recombinase
   rhaS
   rhaR
   

   Expression

   Bacterial

   Promoter

   rhamnose

   Available Since

   Dec. 8, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo

   Plasmid

   #221899

   Purpose

   Expresses alpha tubulin fused to mStayGold

   Depositor

   Janelia Integrative Imaging

   Insert

   mStayGold-aTubulin
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. His-lsMSP1D1

   Plasmid

   #240151

   Purpose

   Expression of the MSP 'His-lsMSP1D1'

   Depositor

   Lise Arleth

   Article

   Tidemand et al Biochim Biophys Acta Biomembr. 2022 Jun

   Insert

   His-lsMSP1D1
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB_EF1a_tNGFR_ZeoR

   Plasmid

   #212648

   Purpose

   piggyBac vector to express tNGFR with zeocin resistance

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   tNGFR
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Mm.cargo(Cas9)

   Plasmid

   #174865

   Purpose

   Expresses mouse Peg10 cargoRNA encoding SpCas9

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   SpCas9 flanked by MmPeg10 UTRs
   

   Tags

   HA-SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRE recombinase

   Plasmid

   #62730

   Purpose

   Expresses Cre recombinase protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   Cre recombinase
   

   Use

   Cre/Lox

   Tags

   His tag and NLS

   Expression

   Bacterial

   Mutation

   Codon optimazed for e.coli

   Promoter

   T7

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-mRFP

    Plasmid

    #13032

    Purpose

    Mammalian expression vector for cloning your gene fused to mRFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Insert

    Monomer Red Fluorescent Protein
    

    Expression

    Mammalian

    Available Since

    Nov. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCE-mp53DD

    Plasmid

    #41856

    Purpose

    Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    Trp53 (Trp53 Mouse)
    

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    CAG

    Available Since

    July 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZT-C13-L1

    Plasmid

    #62196

    Purpose

    Chr.13/CLYBL TALEN expression vector

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    Left CLYBL TALEN
    

    Use

    TALEN

    Promoter

    CMV

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. FRB-mCherry-FTH1

    Plasmid

    #100749

    Purpose

    Part of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tag

    Depositor

    Stephen Royle

    Article

    Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

    Insert

    ferritin heavy chain 1 (FTH1 Human)
    

    Tags

    FRB and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-15F11-HA-mEGFP

    Plasmid

    #129590

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-2xFLAG-2xSTREP-CDK6

    Plasmid

    #172614

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK6 (CDK6 Human)
    

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Red Glifon 300

    Plasmid

    #163115

    Purpose

    Red fluorescent protein-based glucose sensor for mammalian cells with higer affinity

    Depositor

    Takashi Tsuboi

    Article

    Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

    Insert

    Red Glifon 300
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. OpenCRISPR-1

    Plasmid

    #221565

    Purpose

    Express OpenCRISPR-1 nuclease in human cells using a CMV promoter

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1
    

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCDNA3.1-pCMV-PfV-GS-Sapphire

    Plasmid

    #116933

    Purpose

    Mammalian vector for expression of the 40nm GEM

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    Pfv
    

    Tags

    Sapphire

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

    Plasmid

    #71237

    Purpose

    Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A GFP
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSIN-TRE-H2BeGFP-rtTA2

    Plasmid

    #165494

    Purpose

    Doxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.

    Depositor

    Hector G. Palmer

    Article

    Puig et al J Clin Invest. 2018 Aug 31;128(9):3887-3

    Insert

    Human histone H2B (H2BC21 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Mutation

    -

    Promoter

    TetO-CMV

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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