162,717 results

1. pDONR223_RAF1_WT

   Plasmid

   #82317

   Purpose

   Gateway Donor vector containing RAF1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   RAF1 (RAF1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHiUGE GS-gRNA (no Cre)

   Plasmid

   #200370

   Purpose

   Backbone plasmid for cloning HiUGE GS-gRNA (without Cre)

   Depositor

   Scott Soderling

   Article

   Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-SFFV-FLAG-TurboID-SQSTM1

   Plasmid

   #223714

   Purpose

   Expresses Flag-tagged TurboID SQSTM1

   Depositor

   Benjamin Wolozin

   Article

   Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

   Insert

   SQSTM1 (SQSTM1 Human)
   

   Use

   Lentiviral

   Tags

   TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLgw EcoDam-V5-RFC1

   Plasmid

   #59209

   Purpose

   Mammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloning

   Depositor

   Bas van Steensel

   Article

   Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

   Type

   Empty backbone

   Use

   Lentiviral; Damid

   Tags

   Dam and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Heat Shock Minimal Promoter

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. FERM domain of Ezrin

   Plasmid

   #62380

   Purpose

   Expresses the FERM domain of Ezrin (N terminal portion of the protein)

   Depositor

   Guillaume Charras

   Article

   Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

   Insert

   Ezrin aa1-309 (EZR Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains FERM domain of Ezrin (N terminal portion…

   Promoter

   CMV

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDC191

   Plasmid

   #115792

   Purpose

   Expresses Rat synaptobrevin 2

   Depositor

   Axel Brunger

   Article

   Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

   Insert

   Synaptobrevin2 (Vamp2 Rat)
   

   Use

   Tags

   hexa histidine

   Expression

   Bacterial

   Mutation

   S28C and C103S (please see comments below)

   Promoter

   T7

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDC158

   Plasmid

   #115793

   Purpose

   Expresses Rat SNAP25

   Depositor

   Axel Brunger

   Article

   Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

   Insert

   SNAP25 (Snap25 Rat)
   

   Use

   Tags

   hexa histidine

   Expression

   Bacterial

   Mutation

   c84s, c85s, c90s, c92s

   Promoter

   T7

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRVL-3

   Plasmid

   #104581

   Purpose

   Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions

   Depositor

   Daniel Christ

   Article

   Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

   Insert

   Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L234A/L235E/G237A and E318A/K320A/K322A

   Promoter

   CMV

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTMB352_ZFcharm_Kv1_split-inteins

   Plasmid

   #220848

   Purpose

   Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   ZFcharm Kv2
   

   Use

   Lentiviral

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHiUGE GS-gRNA (hSyn-Cre) mTubb3

    Plasmid

    #200369

    Purpose

    Positive control plasmid expressing mouse Tubb3 GS-gRNA (with hSyn-Cre)

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    Mouse Tubb3 gRNA and Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHiUGE smFP-V5 donor ORF2

    Plasmid

    #200382

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. ePB-PURO-TT-PA-CRE

    Plasmid

    #179584

    Purpose

    ePiggyBac vector to conditionally express using DOX a light-inducible Cre-recombinase enzyme that takes advantage of the Magnets dimerization system (Magnet-CRE)

    Depositor

    Ali Hemmati Brivanlou

    Article

    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

    Insert

    PA-MAG-CRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTalpha1-Dre

    Plasmid

    #133925

    Purpose

    Neuron-specific expression of Dre

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    HA-Dre
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. 401 pSG5L

    Plasmid

    #10737

    Depositor

    William Sellers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pSLIK-PUM1-rABE-Flag

    Plasmid

    #191385

    Purpose

    Doxycycline inducible expression of PUM1-rABE

    Depositor

    Stephen Floor

    Article

    Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

    Insert

    PUM1-rABE (PUM1 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    tet-inducible promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-6011nes

    Plasmid

    #108652

    Purpose

    An ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-ERK-EV
    

    Use

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TGFbetaR1 (T204D)-pcw107-V5

    Plasmid

    #64629

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    TGFBR1 (transcript variant 1) (TGFBR1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    T204D

    Promoter

    PGK

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pLENTI EF1alpha FLAG/HA-PALI1

    Plasmid

    #114445

    Purpose

    Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human PALI1 coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    PALI1 (LCOR Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDC216

    Plasmid

    #115791

    Purpose

    Expresses rat Syntaxin 1

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    Syntaxin1 (Stx1a Rat)
    

    Use

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    C145S S193C C271S C272S

    Promoter

    T7

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. epi-BE4max-NG

    Plasmid

    #135977

    Purpose

    Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

    Depositor

    Yongming Wang

    Article

    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only