168,844 results
-
Plasmid#19522DepositorAvailable SinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only
-
qTAG-AAVS1-PGK-Puro-moxGFP
Plasmid#227275PurposeAAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)DepositorInsertAAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-Venus
Plasmid#22665DepositorInsertIRES-Venus
ExpressionMammalianMutationIRES is EMCV IRESAvailable SinceJan. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) mFgfr1 DN (CT#984)
Plasmid#14006DepositorAvailable SinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluu
Plasmid#106122PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR S72T variant
TagsMyc and PDGFRExpressionMammalianMutationChanged Ser 72 to ThrPromotersynapsinAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.GCaMP6m.WPRE.SV40
Plasmid#100838PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1 and AAV9InsertGCaMP6m
UseAAVExpressionMammalianMutationGCaMP3-T302L R303P M378G K379S D380Y T381R S383T …PromoterSynapsinAvailable SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ (w298-1)
Plasmid#22801DepositorTypeEmpty backboneUseRNAi and RetroviralExpressionMammalianAvailable SinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn1-DIO-VGLUT2:miniSOG
Plasmid#214200PurposeFor Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)DepositorInsertSlc17a6 tagged with miniSOG and hemagglutinin
UseAAV and Cre/LoxTagsminiSOG (tagged with HA) is fused to the first in…ExpressionMammalianAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lact-C2-GFP
Plasmid#22852DepositorAvailable SinceJan. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp MCP-mCherry IRES h2b-diRFP
Plasmid#102351PurposeLentiral expression vector bearing the MS2 Coat Protein (MCP) fused to mCherry as well as the nuclear marker histone 2b fused to two copies of irFP. Also bears the hygromycin selection marker.DepositorInsertMS2 Coat Protein
UseLentiviralTagsmCherryPromoterSFFVAvailable SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE2 EGFP
Plasmid#129435Purposeexpress GFPDepositorInsertGFP
ExpressionMammalianAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmVirD2-GFP
Plasmid#90315PurposemVirD2-GFP expression plasmidDepositorInsertA. tumefaciens VirD2 from aa 1 to 204 - GFP
Tags6xHis and GFPExpressionBacterialPromoterT7/T5 IPTG inducibleAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
BbZIP-cALFA
Plasmid#160498PurposeFor the protein expression of BbZIP with ALFA tag at C-terminus in E. coliDepositorInsertBbZIP
TagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Aquamarine-LC3B-TdLanYFP
Plasmid#228560PurposeEncodes the LC3B biosensor where LC3B is flanked by the Aquamarine/TdLanYFP donor/accpetor FRET pair and under the control of the CMV promoterDepositorAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFC331
Plasmid#87843PurposeAMA1 plasmid with Aspergillus optimized Cas9 and argB selection markerDepositorInsertsCas9
argB
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and native pro…Available SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)
Plasmid#114068PurposeT7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guidesDepositorInsertentry vector for LbCas12a crRNAs
UseIn vitro transcriptionAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas1_Cas2/3
Plasmid#89240PurposePlasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1DepositorInsertCas1 and Cas2/3
TagsStrep II tag on Cas1Available SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-haA3A-CBE-VA
Plasmid#220893PurposeExpress haA3A-CBE-VA (with SpCas9) in mammalian cellsDepositorInserthaA3A-CBE-VA
ExpressionMammalianAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDS-Red1-N1 AMPA GluR-B(flip)
Plasmid#109142PurposeThe expression vector carries the red fluorescent protein tagged at the C-terminus of GluA2 subunit flip spliced form of the AMPA receptor.DepositorInsertAMPA receptor
TagsRFPExpressionMammalianMutationGlua2 subunit flip spliced form of the AMPA recep…PromoterCMVAvailable SinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pR2Q358y
Plasmid#101028Purposefluorescent, functional, glutamate receptor subunitDepositorInsertGlutamate receptor subunit (Gria2 Rat)
Tagsfluorescent proteinExpressionMammalianMutationtagged with fluorescent proteinPromoterCMVAvailable SinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only