|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11925||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3806
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
pBS302 carries two directly repeated loxP sites flanking a synthetic DNA sequence designated "STOP." The lox-square STOP cassette sits on a NotI fragment that can excised and gel-purified for injection into fertilized zygotes. The STOP sequence is designed to thwart productive expression of a downstream gene under the control of an upstream promoter (to be inserted in the SfiI-SpeI polylinker region). It will have been removed in cells expressing Cre, or in descendants of cells that previously had expressed Cre, because of Cre-mediated recombination at the loxP sites. The STOP sequence is the same as used to regulate T-Ag expression in pBS241.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS302 was a gift from Brian Sauer (Addgene plasmid # 11925 ; http://n2t.net/addgene:11925 ; RRID:Addgene_11925)
For your References section:Manipulation of transgenes by site-specific recombination: use of Cre recombinase. Sauer B. Methods Enzymol. 1993 . 225():890-900. PubMed 8231893