163,445 results

1. TFORF2593

   Plasmid

   #142336

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   BHLHE40 (BHLHE40 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6xHis-MBP-huMb3Cas12a

   Plasmid

   #174677

   Purpose

   Expresses 6xHis-MBP tagged Mb3Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huMb3Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6xHis-MBP-huMb2Cas12a

   Plasmid

   #174676

   Purpose

   Expresses 6xHis-MBP tagged Mb2Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huMb2Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDA_256

   Plasmid

   #158581

   Purpose

   Lentiviral expression of BE3.9max and customizable gRNA

   Depositor

   John Doench, David Root

   Article

   Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 D10A

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. GZnP3 in pcDNA3.1+

   Plasmid

   #161738

   Purpose

   Cytosolic fluorescent reporter for Zn²⁺ (Kd = 1.3 nM, ex/em = 488 nm/515 nm)

   Depositor

   Yan Qin

   Article

   Minckley et al Nat Commun. 2019 Oct 22;10(1):4806. doi:

   Insert

   GZnP3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIS1

   Plasmid

   #12179

   Depositor

   David Bartel

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBoBi-hTRPV4-C-GC6s

   Plasmid

   #154150

   Purpose

   To detect the calcium ion releases from TRPV4 channel

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

   Insert

   TRPV4 and GCamp6s (TRPV4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLXRN-EGFP

   Plasmid

   #99207

   Purpose

   EGFP expressing bicistronic retroviral vector

   Depositor

   Peter Pedersen

   Insert

   EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   A Kozak sequence was incorporated to enhance tran…

   Promoter

   LTR

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF5-FRT-TagRFP-T-IFT88

   Plasmid

   #61684

   Purpose

   Mammalian expression of IFT88 fused with N terminal TagRFP.T tag

   Depositor

   Maxence Nachury

   Article

   Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

   Insert

   IFT88 (Ift88 Mouse)
   

   Use

   Tags

   TagRFP.T

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXsIP GFP-VPS33A

    Plasmid

    #67022

    Purpose

    human VPS33A ORF was inserted into pMXsIP SPGFP-GS Ci2 vector

    Depositor

    Noboru Mizushima

    Article

    Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

    Insert

    Vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A) (VPS33A Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pLenti-Paxillin EGFP

    Plasmid

    #187688

    Purpose

    In vivo visualization of Paxillin

    Depositor

    Mary Beckerle

    Article

    Smith et al PLoS One. 2013 Aug 21;8(8):e69378. doi:

    Insert

    Paxillin (PXN Chicken)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

    Plasmid

    #176836

    Purpose

    Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    See Depositor Comments Below

    Promoter

    Tet operator (tetracycline inducible expression)

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST47-ARL8B-GFP

    Plasmid

    #67404

    Purpose

    Mammalian expression of hArl8b with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8B (ARL8B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZ M2rtTA_CAGG TetON-Sox10

    Plasmid

    #115240

    Purpose

    RMCE donor vector for inducible expression of SOX10 constitutive expression of m2rtTA (generated from plasmid #112668)

    Depositor

    Catherine Verfaillie

    Article

    Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MiniCoopR mitfa:EGFP

    Plasmid

    #118850

    Purpose

    Expresses EGFP and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    mitfa: EGFP (mitfa Synthetic, Zebrafish)
    

    Use

    Zebrafish expression, tol6

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. human p53-(82-393)

    Plasmid

    #24867

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 82-393

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-tadDE-PcrA M6-UGI

    Plasmid

    #221236

    Purpose

    Express HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    tadDE-PcrA M6-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. FKBP-C-TEV 219

    Plasmid

    #100096

    Purpose

    SNIPer plasmid, C terminal TEV fused to FKBP

    Depositor

    Jim Wells

    Article

    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

    Insert

    C-TEV
    

    Use

    Retroviral

    Tags

    FKBP and Myc

    Expression

    Mammalian

    Mutation

    C terminal domain

    Promoter

    CMV

    Available since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-EF1a-mCherry-MinD-IRES-Puro

    Plasmid

    #213112

    Purpose

    Expression of mCherry-MinD in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    mCherry-MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LRG2.1-BFP

    Plasmid

    #120577

    Purpose

    Lentiviral plasmid to co-express a guide RNA and EBFP.

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge