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Puro-Cas9 donor Citations (13)

Originally described in: An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells.
Gonzalez F, Zhu Z, Shi ZD, Lelli K, Verma N, Li QV, Huangfu D Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018. PubMed Journal

Articles Citing Puro-Cas9 donor

Articles
Generation and characterization of a human iPSC cell line expressing inducible Cas9 in the "safe harbor" AAVS1 locus. Castano J, Bueno C, Jimenez-Delgado S, Roca-Ho H, Fraga MF, Fernandez AF, Nakanishi M, Torres-Ruiz R, Rodriguez-Perales S, Menendez P. Stem Cell Res. 2017 May;21:137-140. doi: 10.1016/j.scr.2017.04.011. Epub 2017 Apr 22. PubMed

Associated Plasmids
Nanoporous Immunoprotective Device for Stem-Cell-Derived beta-Cell Replacement Therapy. Chang R, Faleo G, Russ HA, Parent AV, Elledge SK, Bernards DA, Allen JL, Villanueva K, Hebrok M, Tang Q, Desai TA. ACS Nano. 2017 Aug 22;11(8):7747-7757. doi: 10.1021/acsnano.7b01239. Epub 2017 Aug 7. PubMed
Mitigating Ischemic Injury of Stem Cell-Derived Insulin-Producing Cells after Transplant. Faleo G, Russ HA, Wisel S, Parent AV, Nguyen V, Nair GG, Freise JE, Villanueva KE, Szot GL, Hebrok M, Tang Q. Stem Cell Reports. 2017 Sep 12;9(3):807-819. doi: 10.1016/j.stemcr.2017.07.012. Epub 2017 Aug 10. PubMed
Inducible CRISPR genome editing platform in naive human embryonic stem cells reveals JARID2 function in self-renewal. Ferreccio A, Mathieu J, Detraux D, Somasundaram L, Cavanaugh C, Sopher B, Fischer K, Bello T, M Hussein A, Levy S, Cook S, Sidhu SB, Artoni F, Palpant NJ, Reinecke H, Wang Y, Paddison P, Murry C, Jayadev S, Ware C, Ruohola-Baker H. Cell Cycle. 2018;17(5):535-549. doi: 10.1080/15384101.2018.1442621. Epub 2018 Apr 5. PubMed
Conditional gene knockout and reconstitution in human iPSCs with an inducible Cas9 system. Wu M, Liu S, Gao Y, Bai H, Machairaki V, Li G, Chen T, Cheng L. Stem Cell Res. 2018 May;29:6-14. doi: 10.1016/j.scr.2018.03.003. Epub 2018 Mar 10. PubMed

Associated Plasmids
A Platform for Studying Neurodegeneration Mechanisms Using Genetically Encoded Biosensors. Ustyantseva EI, Medvedev SP, Vetchinova AS, Minina JM, Illarioshkin SN, Zakian SM. Biochemistry (Mosc). 2019 Mar;84(3):299-309. doi: 10.1134/S000629791903012X. PubMed
Tau interactome analyses in CRISPR-Cas9 engineered neuronal cells reveal ATPase-dependent binding of wild-type but not P301L Tau to non-muscle myosins. Wang X, Williams D, Muller I, Lemieux M, Dukart R, Maia IBL, Wang H, Woerman AL, Schmitt-Ulms G. Sci Rep. 2019 Nov 7;9(1):16238. doi: 10.1038/s41598-019-52543-5. PubMed
Loss of the transcription factor MAFB limits beta-cell derivation from human PSCs. Russell R, Carnese PP, Hennings TG, Walker EM, Russ HA, Liu JS, Giacometti S, Stein R, Hebrok M. Nat Commun. 2020 Jun 2;11(1):2742. doi: 10.1038/s41467-020-16550-9. PubMed
Engineering of human mesenchymal stem cells resistant to multiple natural killer subtypes. Zheng D, Wang X, Zhang Z, Li E, Yeung C, Borkar R, Qin G, Wu Y, Xu RH. Int J Biol Sci. 2022 Jan 1;18(1):426-440. doi: 10.7150/ijbs.64640. eCollection 2022. PubMed
Generation of a human extended pluripotent stem cell line (SKLRMe002-A) carrying a doxycycline-inducible Cas9 expression cassette. He Z, Feng K, Sun H, Yu T, Zhu D, Yang Y. Stem Cell Res. 2022 Jul;62:102816. doi: 10.1016/j.scr.2022.102816. Epub 2022 May 17. PubMed
A Tet-Inducible CRISPR Platform for High-Fidelity Editing of Human Pluripotent Stem Cells. Jurlina SL, Jones MK, Agarwal D, De La Toba DV, Kambli N, Su F, Martin HM, Anderson R, Wong RM, Seid J, Attaluri SV, Chow M, Wahlin KJ. Genes (Basel). 2022 Dec 14;13(12):2363. doi: 10.3390/genes13122363. PubMed
Discovery of Competent Chromatin Regions in Human Embryonic Stem Cells. Pulecio J, Tayyebi Z, Liu D, Wong W, Luo R, Damodaran JR, Kaplan S, Cho H, Yan J, Murphy D, Rickert RW, Shukla A, Zhong A, Gonzalez F, Yang D, Li W, Zhou T, Apostolou E, Leslie CS, Huangfu D. bioRxiv. 2023 Jun 14:2023.06.14.544990. doi: 10.1101/2023.06.14.544990. Preprint. PubMed
Genome-wide CRISPRi Screen in Human iNeurons to Identify Novel Focal Cortical Dysplasia Genes. Tidball AM, Luo J, Walker JC, Takla TN, Carvill GL, Parent JM. bioRxiv [Preprint]. 2023 Dec 13:2023.12.13.571474. doi: 10.1101/2023.12.13.571474. PubMed

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