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pQCXI Puro DsRed-LC3-GFP
(Plasmid #31182)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31182 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pQCXIP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DsRed-LC3-GFP
  • Insert Size (bp)
    1850

Cloning Information

Resource Information

Depositor Comments

DsRed-LC3-GFP
autophagy reporter plasmid. The LC3 gene is from rat.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXI Puro DsRed-LC3-GFP was a gift from David Sabatini (Addgene plasmid # 31182 ; http://n2t.net/addgene:31182 ; RRID:Addgene_31182)
  • For your References section:

    Defective Regulation of Autophagy upon Leucine Deprivation Reveals a Targetable Liability of Human Melanoma Cells In Vitro and In Vivo. Sheen JH, Zoncu R, Kim D, Sabatini DM. Cancer Cell. 2011 May 17. 19(5):613-28. 10.1016/j.ccr.2011.03.012 PubMed 21575862