We narrowed to 4,627 results for: pcr
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Plasmid#174026PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertp2A-QF2
UseZebrafish knock-in taggingAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnma1b
Plasmid#164952PurposeZebrafish kcnma1b gene CDSDepositorInsertkcnma1b (kcnma1b Zebrafish)
UsePcr cloning vectorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnmb2a
Plasmid#164953PurposeZebrafish kcnmb2a gene CDSDepositorInsertkcnmb2a (kcnmb2 Zebrafish)
UsePcr cloning vectrorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnmb2b
Plasmid#164954PurposeZebrafish kcnmb2b gene CDSDepositorInsertkcnmb2b (si:ch211-247n2.1 Zebrafish)
UsePcr cloning vectorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnmb3
Plasmid#164955PurposeZebrafish kcnmb3 gene CDSDepositorInsertkcnmb3 (si:ch211-38m6.7 Zebrafish)
UsePcr cloning vectorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnt1b
Plasmid#164962PurposeZebrafish kcnt1b gene CDSDepositorInsertkcnt1b (si:dkey-21e5.1 Zebrafish)
UsePcr cloning vectorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCCG 306
Plasmid#131167PurposePCR template for genomic C-terminal Cub-R-URA3 tag, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutantDepositorTypeEmpty backboneTagsCCGExpressionYeastAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBALU3
Plasmid#69318PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of cfpDepositorInsertCFPint-FgF
ExpressionBacterialAvailable SinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBALU2
Plasmid#69317PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of yfpDepositorInsertYFPint-FgF
ExpressionBacterialAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCY 3120-01
Plasmid#36237DepositorInsertyECitrine-7Myc
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yECitrine-7Myc-kanMXAvailable SinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCY 3120-04
Plasmid#36238DepositorInsertTRP1
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yECitrine-13Myc-TRP1Available SinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
gRNA_GFP-T1
Plasmid#41819PurposeExpresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineeringDepositorInsertgRNA_GFP-T1
UseCRISPRExpressionMammalianAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
gRNA_AAVS1-T2
Plasmid#41818PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineeringDepositorInsertgRNA_AAVS1-T2
UseCRISPRExpressionMammalianAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7RNAP
Plasmid#74096PurposeT7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNADepositorInsertT7 RNAP
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pADH147
Plasmid#90991PurposeNAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.DepositorInsertgRNA conserved, FRT, DS_HIS1
UseCRISPRAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH119
Plasmid#90985PurposeNAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.DepositorInsertgRNA conserved, C. albicans LEU2 2 of 2
UseCRISPRAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-g418-3HA::FRB::3HA
Plasmid#247588PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert3xHA
UseUnspecifiedPromoterread throughAvailable SinceDec. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-g418-3Ty:mNG:3Ty
Plasmid#179800PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNG
UseUnspecifiedPromoterread throughAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
gRNA_AAVS1-T1
Plasmid#41817PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineeringDepositorInsertgRNA_AAVS1-T1
UseCRISPRExpressionMammalianAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template3-3XMyc-Spacer
Plasmid#176210PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template3-3XMyc-Spacer
TagsMyc tagExpressionBacterialPromoterT7Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only