We narrowed to 4,504 results for: pcr

1. human PNP non-tagged

   Plasmid

   #64199

   Purpose

   Expression of native human PNP (non-tagged)

   Depositor

   Vern Schramm

   Article

   Murkin et al Biochemistry. 2007 May 1;46(17):5038-49.

   Insert

   Purine Nucleoside Phosphorylase (PNP Human)
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA_GFP-T2

   Plasmid

   #41820

   Purpose

   Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_GFP-T2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-GBP-mCherry-kanMX6

   Plasmid

   #89068

   Purpose

   genomic GBP-mCherry epitope C-terminal tagging in S. pombe

   Depositor

   Quanwen Jin

   Article

   Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   GBP-mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. A1-20B1(P004)

   Plasmid

   #184056

   Purpose

   This plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.

   Depositor

   C. Ron Yu

   Article

   Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

   Insert

   A1-20B1 preamplifier
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSN007

   Plasmid

   #102440

   Purpose

   For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

   Depositor

   Alejandro Chavez, George Church

   Article

   Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

   Insert

   7SK paired gRNA insert
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPOTv6-puro-3Ty:mNG:3Ty

   Plasmid

   #179798

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Samuel Dean, Keith Gull

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   mNG
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPOTv7-blast-mNG2(1-10)

   Plasmid

   #190209

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Samuel Dean

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   mNeonGreen2(1-10)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. M-ST1-sgRNA

   Plasmid

   #48672

   Purpose

   Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   hU

   Available since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPL5618_pUG_MtxR

   Plasmid

   #60929

   Purpose

   PCR template vector for generating methotrexate resistance cassette.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Dihydrofolate reductase (DHFR Human)
   

   Use

   Loxp flanked deletion cassette template

   Tags

   Expression

   Mutation

   L22F F31S

   Promoter

   TEF1

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPOTv6-puro-3Myc:mScarletI:3Myc

    Plasmid

    #179804

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean, Keith Gull

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    mScarlet-I
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pADH119

    Plasmid

    #90985

    Purpose

    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    gRNA conserved, C. albicans LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJW1354

    Plasmid

    #69490

    Purpose

    Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms

    Depositor

    Jordan Ward

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
    

    Use

    CRISPR; Template to generate cassettes for -media…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pADH147

    Plasmid

    #90991

    Purpose

    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    gRNA conserved, FRT, DS_HIS1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPOTv7-hygro-3HaloTag

    Plasmid

    #179828

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean, Keith Gull

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    3x HaloTag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCCM935

    Plasmid

    #58202

    Purpose

    Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    unc-22 sgRNA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pBx1-VHH template1-3XMyc-Spacer

    Plasmid

    #176208

    Purpose

    PCR template for generating CeVICA VHH/nanobody library

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    VHH template1-3XMyc-Spacer
    

    Use

    Tags

    Myc tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Fret-Stop-Fret TOPO

    Plasmid

    #22774

    Depositor

    Tyler Jacks

    Article

    Jackson et al Genes Dev. 2001 Dec 15. 15(24):3243-8.

    Insert

    fret-STOP-fret
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pgRNA-non-target

    Plasmid

    #129465

    Purpose

    Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Non-target/random gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBx1-VHH template3-3XMyc-Spacer

    Plasmid

    #176210

    Purpose

    PCR template for generating CeVICA VHH/nanobody library

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    VHH template3-3XMyc-Spacer
    

    Use

    Tags

    Myc tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBx1-VHH template2-3XMyc-Spacer

    Plasmid

    #176209

    Purpose

    PCR template for generating CeVICA VHH/nanobody library

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    VHH template2-3XMyc-Spacer
    

    Use

    Tags

    Myc tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge