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pLX304_zeo_mmFbxw7(res)

Plasmid

#160106

Purpose

Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7alpha (Fbxw7 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF2F04

Plasmid

#70901

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

mod(mdg4) (mod(mdg4) Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pCambia-loxKan

Plasmid

#199590

Purpose

basic plant vector with a plant-selectable kanamycin resistance cassette flanked by both loxP and FRT sites for sustainable plant engineering with subsequent marker removal possibility

Depositor

Andreas Stuermer

Insert

FRT-lox66-NosP-KanR-NosT-lox71-FRT

Use

Cre/Lox

Expression

Plant

Promoter

Nos

Available Since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pET21b-C-MBP

Plasmid

#214304

Purpose

Provide a bacterial expression vector that encodes C-terminus MBP tag

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Type

Empty backbone

Expression

Bacterial

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJK295

Plasmid

#71561

Purpose

Production of Acetobacter aceti 1023 thioredoxin (AaTrx)

Depositor

T. J. Kappock

Article

Starks et al Protein Sci. 2007 Jan;16(1):92-8.

Insert

thioredoxin

Expression

Bacterial

Promoter

T7 promoter

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PE-SUMO CRYGA

Plasmid

#217672

Purpose

For expression of SUMO tagged human gamma A crystallin

Depositor

Larry David

Insert

CRYGA (CRYGA Human)

Tags

6XHis and Smt3

Expression

Bacterial

Promoter

T7

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETB BS2mT4L1

Plasmid

#141203

Purpose

Chimeric protein of inactive T4L lysozyme and an estarase from Bacillus subtilis.TEV cleaving site after the N-terminal Histag.

Depositor

Francesca Paradisi

Article

Planchestainer et al Catalysts 2018, 8(1), 40

Insert

BS2m-T4L1

Expression

Bacterial

Mutation

E11Q

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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pTORGm43GW

Plasmid

#112902

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-IcaR-e

Plasmid

#51176

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

IcaR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pRL-ubi63E

Plasmid

#74280

Purpose

Renilla control plasmid driven by the ubiquitin 63E promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Insert

Renilla luciferase

Expression

Insect

Promoter

ubiquitin 63E promoter

Available Since

April 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWY

Plasmid

#112906

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mCitrine.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAMS366-4xCDRE-GFP

Plasmid

#138657

Purpose

stable GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiae

Depositor

Sabrina Büttner

Article

Diessl et al Microbial Cell, 2020: in press

Insert

4xCDRE-CYC1(PM)-yeGFP

Use

Yeast reporter plasmid

Promoter

CYC1

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWR

Plasmid

#112905

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of tdTomato.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWC

Plasmid

#112903

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mTFP1.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWF

Plasmid

#112904

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mWasabi.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRF-QacR

Plasmid

#49369

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

QacR repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-ClfB_N2N3-HIS-ybbr

Plasmid

#101717

Purpose

ClfB N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

ClfB_N2N3

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pRF-IcaR

Plasmid

#49362

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

IcaR repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pGGF001

Plasmid

#48842

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::BastaR::tMAS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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SaMscL (CDelta26)

Plasmid

#79027

Purpose

Staphylococcus aureus MscL with C Terminal Truncation of 26 amino acids

Depositor

Douglas Rees

Article

Liu et al Nature. 2009 Sep 3;461(7260):120-4. doi:

Insert

MscL

Tags

6x Histidine Tag

Mutation

Stop codon placed before 26 amino acids at C Term…

Promoter

T7 Promoter

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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