Addgene

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pEERM4 Cm

Plasmid

#64026

Purpose

Plasmid for integration into neutral site 2 of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PnrsB

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pEERM3 Km

Plasmid

#64025

Purpose

Plasmid for integration into neutral site of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PnrsB

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pEERM1 Km

Plasmid

#64024

Purpose

Plasmid for integration into psbA2 site of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PpsbA2

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pzk384-pmax-PUFc-FseI-RESCUE-SalI-NES

Plasmid

#196831

Purpose

Express PUFc-RESCUE-S

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

PUFc-RESCUE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEERM6 Km

Plasmid

#64027

Purpose

Plasmid for integration into sqs site of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PnrsB

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-Aa-artsg13 (BsaI)

Plasmid

#188279

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

Insert

Aa-artsg13

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

U6

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pmax-hcoRBFOX1N-PUFc-RBFOX1C

Plasmid

#196830

Purpose

Express hcoRBFOX1N-PUFc-RBFOX1C(190-397)

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

hcoRBFOX1N-PUFc-RBFOX1C(190-397)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pmax-hcoRBFOX1N-PCP-RBFOX1C

Plasmid

#196838

Purpose

Express hcoRBFOX1N-PCP-RBFOX1C(190-397)

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

hcoRBFOX1N-PCP-RBFOX1C(190-397)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pmax-hcoRBFOX1N-MCP-RBFOX1C

Plasmid

#196839

Purpose

Express hcoRBFOX1N-MCP-RBFOX1C(190-397)

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

hcoRBFOX1N-MCP-RBFOX1C(190-397)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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EF.CMV.RFP

Plasmid

#17619

Depositor

Linzhao Cheng

Article

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pZZMP122

Plasmid

#231756

Purpose

Mammalian expression of SPO11-YF

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

SPO11-YF (Spo11 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Y138F

Promoter

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pDA303

Plasmid

#195199

Purpose

Overexpressing 6His-TetR in E. coli.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

TetR

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T5 promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-tRNAx2

Plasmid

#123641

Purpose

Mammalian expression, Genome editing, backbone

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pOG2003

Plasmid

#49516

Purpose

TALEN backbone for expression of TALE(deltaRDV)-FokI RR in C. elegans

Depositor

Guangshuo Ou

Article

Cheng et al Nat Biotechnol. 2013 Oct;31(10):934-7. d

Type

Empty backbone

Use

Tags

FokI-RR, HA, and SV40NLS

Expression

Worm

Mutation

Promoter

hsp-16.2

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pOG2002

Plasmid

#49514

Purpose

TALEN backbone for expression of TALE(deltaRDV)-FokI AS in C. elegans

Depositor

Guangshuo Ou

Article

Cheng et al Nat Biotechnol. 2013 Oct;31(10):934-7. d

Type

Empty backbone

Use

Tags

3xFLAG, FokI-AS, and SV40 NLS

Expression

Worm

Mutation

Promoter

hsp-16.2

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pZZMP132

Plasmid

#231757

Purpose

Mammalian expression of SPO11-EA

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

SPO11-EA (Spo11 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

E224A

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-Bs3-sgRNA (BsaI)

Plasmid

#188280

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

Insert

Bs3-sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

U6

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-NE2h

Plasmid

#208687

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neurons

Depositor

Yulong Li

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2h

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-NE2m

Plasmid

#208686

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neurons

Depositor

Yulong Li

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2m

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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mIL12-N1

Plasmid

#123139

Purpose

Mammalian expression of murine cytokine IL-12 under CMV promoter

Depositor

Jordan Green

Article

Tzeng et al Proc Natl Acad Sci U S A. 2020 Feb 7. pi

Insert

Murine IL12 tandem p40 p35

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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