We narrowed to 33,233 results for: eng
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Plasmid#155317PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationS135APromoterM13Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
CALID
Plasmid#169708PurposeCre-activated light-inducible Dre recombinaseDepositorInserta double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
ExpressionMammalianMutationnonePromoterCAGAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRGEB32-CrU6-2-35S
Plasmid#226706PurposeFor CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from CaMV 35S promoter.DepositorInsertCrU6-2
UseCRISPRAvailable SinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRGEB32-CrActin-35S
Plasmid#226708PurposeFor CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from CaMV 35S promoter.DepositorInsertCrActin
UseCRISPRAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRGEB32-CrActin-UBI
Plasmid#226709PurposeFor CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.DepositorInsertCrActin
UseCRISPRAvailable SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRGEB32-CrU6-2-UBI
Plasmid#226707PurposeFor CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.DepositorInsertCrU6-2
UseCRISPRAvailable SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
eNS2B47NS3 S135A
Plasmid#155318PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker S135A mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationS135APromoterM13Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF.GFP
Plasmid#17616DepositorAvailable SinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
Td-CBEmax
Plasmid#196600PurposeMammalian expression of Td-CBEmaxDepositorInsertbpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
Tags6*HisExpressionMammalianMutationTadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)Available SinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pW34
Plasmid#158210PurposeLuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites.DepositorInsertLuxABCDE
Available SinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3
Plasmid#213973PurposeAAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locusDepositorInsertShank3 sgRNA
UseAAV and CRISPRAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
eTd-CBEm
Plasmid#196602PurposeMammalian expression of eTd-CBEmDepositorInsertbpNLS-TadA-8e (N46L/A48M)-nSpCas9-bpNLS-P2A-UGI-bpNLS
Tags6*HisExpressionMammalianAvailable SinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pW5Y_Apr
Plasmid#158211PurposeBAC-based, lacUV5 regulon and Cre recombinase in loxP and lox5171 sitesDepositorTypeEmpty backboneAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pS-sg:GFP
Plasmid#196296Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSsDepositorInsertFull length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
UseCRISPRExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pM-Cas9
Plasmid#196325Purpose35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GPDepositorInsertFull length TSWV M antigenome encoding SpCas9 in place of viral GP
UseCRISPRTags3×flagExpressionPlantMutationSee depositor comments belowPromoterduplicated cauliflower mosaic virus 35S promoterAvailable SinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pM-ABE
Plasmid#196292Purpose35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GPDepositorInsertFull length TSWV M antigenome encoding ABE in place of viral GP
UseCRISPRTags3×flagExpressionPlantMutationSee depositor comments belowPromoterduplicated cauliflower mosaic virus 35S promoterAvailable SinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pM-Cas12a
Plasmid#196291Purpose35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GPDepositorInsertFull length TSWV M antigenome encoding LbCas12a in place of viral GP (cas12a Synthetic)
UseCRISPRTagsFlagExpressionPlantMutationSee depositor comments belowPromoterduplicated cauliflower mosaic virus 35S promoterAvailable SinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS-cr:GFP
Plasmid#196295Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSsDepositorInsertFull length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
UseCRISPRTagsSpeI-DR-BsaI-DR-SpeIExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTK Cas9-2A-Citrine
Plasmid#92393PurposeEnhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.DepositorInsertCas9 2A Citrine
UseCRISPRExpressionMammalianPromoterthymidine kinase promoterAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
eTd-CBE
Plasmid#196601PurposeMammalian expression of eTd-CBEDepositorInsertbpNLS-TadA-8e (N46L)-nSpCas9-bpNLS-P2A-UGI-bpNLS
Tags6*HisExpressionMammalianAvailable SinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only