We narrowed to 2,508 results for: BFP

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pKLV2-U6gRNA5(Tcf7l1-g1)-PGKpuroBFP-W

Plasmid

#105015

Purpose

Lentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

gRNA targeting Tcf7l1 (Tcf7l1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Tcf7l1-g2)-PGKpuroBFP-W

Plasmid

#105016

Purpose

Lentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tcf7l1 (Tcf7l1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Kcmf1-g1)-PGKpuroBFP-W

Plasmid

#105017

Purpose

Lentiviral gRNA plasmid targeting mouse Kcmf1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Kcmf1 (Kcmf1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Pggt1b-g1)-PGKpuroBFP-W

Plasmid

#105018

Purpose

Lentiviral gRNA plasmid targeting mouse Pggt1b , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Pggt1b (Pggt1b Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Znrf1-g1)-PGKpuroBFP-W

Plasmid

#105020

Purpose

Lentiviral gRNA plasmid targeting mouse Znrf1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Znrf1 (Znrf1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Nod-BFP2-TSERex

Plasmid

#124785

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

Nod-BFP2

Expression

Mammalian

Promoter

CAG

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

Plasmid

#84257

Purpose

Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sp dCas9-ABI
GID1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJT241 Lenti pEF-dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast

Plasmid

#187326

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT342 Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

Plasmid

#195541

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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EK0387 SFFV-mTagBFP2-SGc(Bdnf.s1)cSG-mCherry (FLP-IN)

Plasmid

#191157

Purpose

Expression of a sensor for murine Bdnf 3' UTR in mammalian cells; mTagBFP2 marker, mCherry output; 90 bp long

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mTagBFP2:Bdnf.s1:mCherry

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187950

Purpose

ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TO-NFIA.1-SOX9 (bi-directional promoter)

Plasmid

#182306

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

NFIA (NFIA Human)
SOX9 (SOX9 Human)
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

TRE3G-Bi-directional

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHIE050 ZF43x0 mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138731

Purpose

Integration Vector, assembled with pHIE042 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x0

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKG01418_(TRE-TagBFP-bGH)-EF1alpha-mRuby2-SV40

Plasmid

#252543

Purpose

Inducible gene and constitutive reporter with divergent syntax, used to generate monoclonal PiggyBac-integrated lines

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

mRuby2

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), Tight TR…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pB-EFS-FastFUCCI-IRES-3xnls-mTagBFP2 (JDW 1515)

Plasmid

#242600

Purpose

A PiggyBac expression vector containing the core promoter from human EF1a driving FastFUCCI to label cell cycle state followed by an IRES-3xnls-mTagBFP2.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

human EF1a

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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VEE-mScarlet3-IRES-E3-NSs-L*-EGFP-IRES-moxBFP

Plasmid

#242410

Purpose

Self-amplifying RNA (saRNA) construct expresses dsRNA pathway inhibitors (vaccinia virus E3, Toscana virus NSs, Theiler’s virus L*) and moxBFP (in place of inflammatory signaling inhibitors).

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

mScarlet3
IRES-E3-NSs-L*-EGFP
IRES-moxBFP

Use

Mouse Targeting; Self-amplifying rna

Expression

Mammalian

Available Since

Sept. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pRhobin2-H2B-mNG3K-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239020

Purpose

Co-overexpression of Rhobin2-H2B-mNeonGreen3K fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

Rhobin2-H2B (H2BC21 Synthetic, Human)

Tags

TagBFP and mNeonGreen3K

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

Plasmid

#228252

Purpose

pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

SpCas9 P23H sgRNA, mTagBFP2

Expression

Mammalian

Mutation

n/a

Promoter

CMV and U6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Plasmid

#221076

Purpose

SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Plasmid

#221077

Purpose

SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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