165,744 results

1. pdCas9_CL

   Plasmid

   #166245

   Purpose

   regulated dCas9 generator

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

   Insert

   P112-sgRNA-term-P104-RBS1-dCas9-B15
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ec-TTL-P112 and Ec-TTL-P104

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHBS1392 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaNTD]

   Plasmid

   #118807

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of NTD in full-length TDP43

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 4QOX

   Plasmid

   #61959

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PP-CDPK4
   

   Use

   Baculovirus expression

   Tags

   MHHHHHHSSGRENLYFQG

   Mutation

   See comments

   Promoter

   Polyhedrin

   Available Since

   May 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. RP185 pUAST EGFP:Or47b

   Plasmid

   #59525

   Purpose

   N-terminal fusion of GFP to Drosophila melanogaster Or47b

   Depositor

   Leslie Vosshall

   Article

   Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

   Insert

   EGFP:Or47b (Or47b Fly)
   

   Tags

   EGFP

   Expression

   Insect

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

5. RP132 pUAST EGFP:Or43a

   Plasmid

   #59523

   Purpose

   N-terminal fusion of EGFP to Drosophila melanogaster Or43a

   Depositor

   Leslie Vosshall

   Article

   Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

   Insert

   EGFP:Or43a (Or43a Fly)
   

   Tags

   EGFP

   Expression

   Insect

   Available Since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pMax_GFP

   Plasmid

   #177825

   Purpose

   Host Cell Reactivation (HCR) control GFP plasmid (pMax backbone)

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   GFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCCL-CellCycle

   Plasmid

   #132429

   Purpose

   Expressing Fucci cell cycle indicator in mammalian cells

   Depositor

   John M. Greally

   Article

   Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

   Insert

   hCdt, hGeminin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-GRAB-gDA3m

   Plasmid

   #208698

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Has Service

   AAV1

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_gDA3m
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1-G3BP1-WT

   Plasmid

   #135997

   Purpose

   WT G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJYS3_ΔcrtYf

    Plasmid

    #85542

    Purpose

    Constitutive transcription of FnCpf1 and crRNA of crtYf

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Expression

    Bacterial

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBbi-RB

    Plasmid

    #60522

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpRY Cas9

    Plasmid

    #188491

    Purpose

    Expresses FLAG tagged SpRY Cas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAF0035 Ef1a-Nm60-2a-EGFP

    Plasmid

    #193464

    Purpose

    Expression of Nm60 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Nm60
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJYS1Ptac

    Plasmid

    #85545

    Purpose

    Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    recT
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Expression

    Bacterial

    Promoter

    tac and unknown

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-TRE-EGFP-EF1a-rtTA

    Plasmid

    #104454

    Purpose

    PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

    Depositor

    Volker Busskamp

    Article

    Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

    Inserts

    enhanced green fluorescent protein
    reverse tetracycline-controlled transactivator 3
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEM705 Gsq

    Plasmid

    #109356

    Purpose

    GsX chimera with Gαq C-terminus

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gsq chimera
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAF0084 Ef1a-Cp36-2a-EGFP

    Plasmid

    #193462

    Purpose

    Expression of Cp36 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-BRCA1

    Plasmid

    #115491

    Purpose

    EGFP-BRCA1 expression vector. Parton clone BXY

    Depositor

    Rob Parton

    Article

    McMahon et al Elife. 2021 Jun 18;10. pii: 61407. doi:

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S1009R (See depositor comments)

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSIN-PAmCherry-KFERQ-NE

    Plasmid

    #102365

    Purpose

    Lentiviral overexpression of PA-mCherry-KFERQ fusion

    Depositor

    Shu Leong Ho

    Article

    Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

    Inserts

    PA-mCherry
    KFERQ peptide
    

    Use

    Lentiviral

    Tags

    NE

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid

    #138147

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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