163,606 results

1. pNCS YFP

   Plasmid

   #91759

   Purpose

   Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

   Insert

   Venus YFP (A206K)
   

   Use

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. amilCP_Pink chromoprotein

   Plasmid

   #117851

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Pink chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amilCP_Pink
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   64A,65C, BioBrick sites removed

   Promoter

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PxylA

   Plasmid

   #55178

   Purpose

   Xylose-inducible promoter

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   xylA promoter
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   xylA

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIG-602_NY-ESO-1_TCR_RFP

   Plasmid

   #207483

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   RFP, NY-ESO-1_TCR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO V5 HSPA8

   Plasmid

   #19514

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 8 (HSPA8 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. hFerritinL

   Plasmid

   #122653

   Purpose

   Expression of hFerritinL in bacterial cells

   Depositor

   Trevor Douglas

   Insert

   light chain ferritin, human (FTL Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-DIO-GRAB-gDA3m

   Plasmid

   #208707

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_gDA3m
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-IP-mVenus-TOSI

   Plasmid

   #172491

   Purpose

   mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

   Depositor

   David Scadden

   Article

   Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

   Insert

   Pdcd4 (Pdcd4 Mouse)
   

   Use

   Retroviral

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   MLV LTR

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-IRES-GFP/CREB3L1

   Plasmid

   #214767

   Purpose

   Mammalian expression of human CREB3L1

   Depositor

   Norio Komatsu

   Article

   De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

   Insert

   CREB3L1 (CREB3L1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   MSCV

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-TRE-eYFP

    Plasmid

    #104056

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein eYFP

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACYCDuet-1:ChrA

    Plasmid

    #225079

    Purpose

    Expresses precursor peptide ChrA in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrA
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAW014-2

    Plasmid

    #85612

    Purpose

    Multi-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilis

    Depositor

    Perry Chou

    Article

    Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

    Insert

    ugtP-gRNA.P395T
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEIAV-SIN6.1 CGFPW

    Plasmid

    #44171

    Depositor

    John Olsen

    Article

    McKay et al Gene Ther. 2006 Apr;13(8):715-24.

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    chromophore mutations

    Promoter

    CMV

    Available Since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPICZalphaB-SapL3

    Plasmid

    #78171

    Purpose

    Expression of Saporin L3 in pichia

    Depositor

    Vern Schramm

    Article

    Yuan et al Biochemistry. 2015 Jul 28;54(29):4565-74

    Insert

    Saporin L3
    

    Use

    Tags

    alpha factor

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pK27Sumo_His-SUMO-PLpro (nsp3) (SARS-CoV-2)

    Plasmid

    #169193

    Purpose

    To express SARS-CoV-2 Papain-like protease in E. coli

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_BiPVe4_Gq_dTomato

    Plasmid

    #213938

    Purpose

    AAV construct expressing HA-hM3Dq-dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_Gq_dTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    HA-GqDREADD-dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACλCI-β-flap (831-1057)

    Plasmid

    #53733

    Purpose

    Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    λCI-β-flap fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac UV5

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBRα-β flap (831-1057)

    Plasmid

    #53734

    Purpose

    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    alpha NTD-Beta flap fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET41b+_Npl4

    Plasmid

    #117108

    Purpose

    Production of recombinant Npl4 in E.coli

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    Npl4 (NPLOC4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDRF1-GW ymNeongreen-T2A-mCherry

    Plasmid

    #125700

    Purpose

    Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymNeongreen-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge