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pCMV-ZFN-AAVS1

Plasmid

#89707

Purpose

Mammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locus

Depositor

Dmitriy Mazurov

Article

Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

Insert

ZFN-AAVS1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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murine TCR OTI-2A.pMIG II

Plasmid

#52111

Purpose

retroviral expression of both TCRalpha and TCRbeta using a single vector

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Inserts

T cell receptor alpha chain (Tcra Mouse)
T cell receptor beta chain (Tcrb Mouse)

Use

Retroviral

Tags

T2A (from Thosea asigna virus)

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-HP1beta

Plasmid

#17651

Depositor

Tom Misteli

Article

Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

Insert

HP1 beta (CBX1 Human)

Tags

GFP and His

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pNF02-mLemon

Plasmid

#219399

Purpose

Constitutive expression of mLemon in E. coli

Depositor

Christian Lesterlin

Article

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mLemon

Expression

Bacterial

Promoter

proDp

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pFZ19

Plasmid

#36184

Purpose

Expression of genes under control of an estrogen-inducible 35S promoter

Depositor

Daniel Voytas

Article

Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

Type

Empty backbone

Use

Plant t-dna vector

Expression

Plant

Promoter

XVE_35S (estrogen-inducible)

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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plenti6-SLC7A11/xCT-V5

Plasmid

#170427

Purpose

Eepresses SLC7A11 (also named xCT) in mammalian cells

Depositor

Boyi Gan

Article

Koppula et al J Biol Chem. 2017 Aug 25;292(34):14240-1

Insert

solute carrier family 7 member 11 (SLC7A11 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-neo largeTcDNA

Plasmid

#1780

Purpose

Retroviral expression of Large T Antigen. Used to create immortalized cell lines.

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

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pSIRV-NF-kB-eGFP

Plasmid

#118093

Purpose

Fluorescent reporter for NF-kB activity

Depositor

Peter Steinberger

Article

Jutz et al Oncotarget. 2017 May 4;8(39):64892-64906

Insert

eGFP

Use

Retroviral

Promoter

minimal promoter with NF-kB responsive element

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-GFP

Plasmid

#222996

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-EGFP

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1)

Plasmid

#17452

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_ACh3.0

Plasmid

#121923

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Promoter

hSyn

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2

Plasmid

#135504

Purpose

Mammalian expression of myc-tagged HLA-A*02:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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phCMV-coGALVenv-C4070A

Plasmid

#172217

Purpose

Expresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectors

Depositor

Boris Fehse

Article

Mirow et al Viruses. 2021 Jul 27;13(8). pii: v130814

Insert

Envelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)

Expression

Mammalian

Mutation

Carries C-terminus of amphotropic MLV strain 4070…

Promoter

CMV immediate early promoter

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD25

Plasmid

#231547

Purpose

L-arabinose inducible expression of heterologous genes, pBAD24-based backbone with KanR selective marker

Depositor

Martin Wiedmann

Article

Schumann et al J Microbiol Methods. 2025 Mar 18;232-234

Type

Empty backbone

Expression

Bacterial

Promoter

pBAD

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCuo CA Rac1

Plasmid

#84643

Purpose

constitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

Rac1 (RAC1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Constitutively Active, Q61L

Promoter

cumate-inducible promoter

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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anti-FLAG M2 heavy chain

Plasmid

#175359

Purpose

Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

Depositor

Joost Snijder

Article

Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

Insert

anti-FLAG M2 heavy chain

Tags

His8 tag and N-terminal secretion signal peptide …

Expression

Mammalian

Mutation

L51I (see paper)

Promoter

CMV

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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NLS-mCherry-LEXY (pDN122)

Plasmid

#72655

Purpose

Blue light-inducible nuclear export of mCherry

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

NLS-mCherry-LEXY

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCE-mp53DD

Plasmid

#41856

Purpose

Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

Trp53 (Trp53 Mouse)

Expression

Mammalian

Mutation

Δ40-903

Promoter

CAG

Available Since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-MITF-A

Plasmid

#38132

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

MITF-A (MITF Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only

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p53-TRE-mStrawberry reporter

Plasmid

#158684

Purpose

This plasmid is a p53 pathway reporter. It has two copies of wild-type p53 binding sites promoter driving the expression of mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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