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A Transcription Factor Addiction in Leukemia Imposed by the MLL Promoter Sequence.
Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G, Mackay JP, Kinney JB, Shi J, Vakoc CR
Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
118636Lenti_SaCRISPR_GFPTo clone sgRNA for SaCas9
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118650Lenti_sgRNA_MS2_neoTo clone sgRNA for activation dCas9
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118661LentiV_Neo_WT_ZFP64Express human wide type ZFP64 gene
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118692pHAGE_puroEmpty vector to express overexpression cDNA
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118693pHAGE_puro_ZFP64_N_3XFLAGExpress 3xflag tagged ZFP64 for flag ChIP experiments
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118694Lenti_Neo_WT_ZFP64_CRExpress sgZFP64e2 resistant cDNA
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118695pGL421_MLL_promoterExpress MLL/KMT2A promoter for luciferase assay
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118696pGL421_MLL2_promoterExpress MLL2/KMT2B promoter for luciferase assay
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118698LentiV_Neo_ZFP64_Delta_TADExpress ZFP64 without transcription activation domain(TAD)
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118699Lenti_MS2_P65_HSF1_2A_puroTo express MS2-P65-HSF1
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118700Lenti_Neo_ZFP64_N_3XFLAGExpress 3xflag tagged ZFP64
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Recombinant Antibodies from Article