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A Transcription Factor Addiction in Leukemia Imposed by the MLL Promoter Sequence.
Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G, Mackay JP, Kinney JB, Shi J, Vakoc CR
Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
118636Lenti_SaCRISPR_GFPTo clone sgRNA for SaCas9 (SauCas9)
118650Lenti_sgRNA_MS2_neoTo clone sgRNA for activation dCas9
118661LentiV_Neo_WT_ZFP64Express human wild type ZFP64 gene
118692pHAGE_puroEmpty vector to express overexpression cDNA
118693pHAGE_puro_ZFP64_N_3XFLAGExpress 3xflag tagged ZFP64 for flag ChIP experiments
118694Lenti_Neo_WT_ZFP64_CRExpress sgZFP64e2 resistant cDNA
118695pGL421_MLL_promoterExpress MLL/KMT2A promoter for luciferase assay
118696pGL421_MLL2_promoterExpress MLL2/KMT2B promoter for luciferase assay
118698LentiV_Neo_ZFP64_Delta_TADExpress ZFP64 without transcription activation domain(TAD)
118699Lenti_MS2_P65_HSF1_2A_puroTo express MS2-P65-HSF1
118700Lenti_Neo_ZFP64_N_3XFLAGExpress 3xflag tagged ZFP64

Antibodies from Article