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pcDNA3-EGFP-Cdc42-wt

Plasmid

#12975

Depositor

Gary Bokoch

Publication

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pIS0

Plasmid

#12178

Purpose

3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production

Depositor

David Bartel

Publication

Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

33 Articles

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SpyCatcher003-sfGFP

Plasmid

#133449

Purpose

Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003-sfGFP

Tags

His6 and TEV site

Expression

Bacterial

Promoter

T5

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pMA3790

Plasmid

#70110

Purpose

mtDNA elimination/production of rho-0 cells

Depositor

Mikhail Alexeyev

Publication

Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

Inserts

EGFP
hUNG1 Y147A
Neo

Tags

None, human OTC mitochondrial targeting sequence,…

Expression

Mammalian

Mutation

Y147A, aa's 1-77 removed

Promoter

CMV, EF1a, and SV40 and native

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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L304-EGFP-Tubulin-WT

Plasmid

#64060

Purpose

EGFP fused at the N-terminus of tubulin; lentiviral construct

Depositor

Weiping Han

Publication

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

Plasmid

#51509

Purpose

Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Publication

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has service

AAV1

Insert

tdTomato-T2A-SypEGFP

Use

AAV

Promoter

phSyn1

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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pK7WGF2::hCas9

Plasmid

#46965

Purpose

Expresses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

Depositor

Sophien Kamoun

Publication

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

hCas9

Use

CRISPR; Plant expression

Tags

eGFP

Promoter

35S

Available since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pZW6 (CK2alpha)

Plasmid

#27086

Depositor

David Litchfield

Publication

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha (CSNK2A1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pCXLE-hMLN

Plasmid

#27079

Purpose

Non-integrating (episomal) expression of human C-MYC, LIN28 and NANOG

Depositor

Shinya Yamanaka

Publication

Okita et al Nat Methods. 2011 May;8(5):409-12

Insert

C-MYC, LIN28, NANOG (MYC Human)

Expression

Mammalian

Available since

May 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pMXs-gw

Plasmid

#18656

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Gateway cassette A

Use

Retroviral

Expression

Mammalian

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pCAGGS-ChR2-Venus

Plasmid

#15753

Purpose

Expresses ChR2-Venus for neuronal excitation.

Depositor

Karel Svoboda

Publication

Petreanu et al Nat Neurosci. 2007 May . 10(5):663-8.

Insert

Channelrhodopsin2-Venus

Tags

Venus

Expression

Mammalian

Promoter

CAG

Available since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pIRESneo-FLAG/HA Ago4

Plasmid

#10824

Depositor

Thomas Tuschl

Publication

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

Argonaute 4 (AGO4 Human)

Tags

FLAG and HA

Expression

Mammalian

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pcDNA3.1_FASN

Plasmid

#107138

Purpose

His tagged human FASN cloned in pcDNA3.1 vector for expression in mammalian cells

Depositor

Brijesh Kumar Singh

Insert

Fatty acid synthase (FASN Human)

Tags

His

Expression

Mammalian

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHaloTag-EGFP

Plasmid

#86629

Purpose

Expresses HaloTag-eGFP tandem (cytosolic)

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

HaloTag

Expression

Mammalian

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pRL443

Plasmid

#70261

Purpose

Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.

Depositor

Peter Wolk

Publication

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Type

Empty backbone

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-Tet2

Plasmid

#60939

Purpose

Expresses Tet2 in mammalian cells

Depositor

Yi Zhang

Publication

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 2 (Tet2 Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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Lck-GFP

Plasmid

#61099

Purpose

Expression of a membrane-targeted form of eGFP

Depositor

Steven Green

Publication

Benediktsson et al J Neurosci Methods. 2005 Jan 30;141(1):4

Insert

Lck-GFP

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pcDNA3-flag-YTHDF2

Plasmid

#52300

Purpose

full length YTHDF2 with N-terminal flag tag for mammalian cell expression

Depositor

Chuan He

Publication

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

flag

Expression

Mammalian

Promoter

T7

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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p16sheLL

Plasmid

#37320

Depositor

John Schiller

Publication

Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

Insert

HPV16L1+L2

Expression

Mammalian

Promoter

CMV

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pSLIK-Venus

Plasmid

#25734

Purpose

Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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