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pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

Plasmid

#188768

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEG BacMam

Plasmid

#160451

Purpose

vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Tet2

Plasmid

#60939

Purpose

Expresses Tet2 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 2 (Tet2 Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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4XCLEAR-luciferase reporter

Plasmid

#66800

Purpose

Reporter for activity of the CLEAR network

Depositor

Albert La Spada

Article

Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

Insert

4XCLEAR

Use

Luciferase

Promoter

Four CLEAR elements (GTCACGTGAC) in tandem derive…

Available Since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

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Lck-GFP

Plasmid

#61099

Purpose

Expression of a membrane-targeted form of eGFP

Depositor

Steven Green

Article

Benediktsson et al J Neurosci Methods. 2005 Jan 30;141(1):4

Insert

Lck-GFP

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-flag-YTHDF2

Plasmid

#52300

Purpose

full length YTHDF2 with N-terminal flag tag for mammalian cell expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

flag

Expression

Mammalian

Promoter

T7

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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p16sheLL

Plasmid

#37320

Depositor

John Schiller

Article

Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

Insert

HPV16L1+L2

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pZW6 (CK2alpha)

Plasmid

#27086

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha (CSNK2A1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCI-SEP-NRI

Plasmid

#23999

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

NR1 (Grin1 Rat)

Tags

NR1 signal sequence (aa1-20) and SEP (supereclipt…

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pGP-FB-orig BA

Plasmid

#13420

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

orig BA

Tags

Gal11P

Expression

Bacterial

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pKH3

Plasmid

#12555

Depositor

Ian Macara

Article

Mattingly et al Nature. 1996 Jul 18. 382(6588):268-72.

Type

Empty backbone

Tags

3xHA

Expression

Mammalian

Available Since

Oct. 2, 2006

Availability

Academic Institutions and Nonprofits only

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pBABE puro SDF-1 alpha

Plasmid

#12270

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

SDF-1 alpha (CXCL12 Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pCruzHA SIRT1

Plasmid

#10962

Depositor

Toren Finkel

Article

Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

Insert

SIRT1 (Sirt1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-SARS2-B.1.617.2

Plasmid

#172320

Purpose

expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike of B.1.617.2 strain (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

Promoter

CMV-F

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSL1142 (pSPIN, pBBR1 backbone)

Plasmid

#160730

Purpose

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003-sfGFP

Plasmid

#133449

Purpose

Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003-sfGFP

Tags

His6 and TEV site

Expression

Bacterial

Promoter

T5

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-MAPPER

Plasmid

#117721

Purpose

MAPPER Selectively Labels ER-PM Junctions

Depositor

Jen Liou

Article

Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

Insert

MAPPER

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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L304-EGFP-Tubulin-WT

Plasmid

#64060

Purpose

EGFP fused at the N-terminus of tubulin; lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP Centrin2 (Nigg UK185)

Plasmid

#41147

Depositor

Erich Nigg

Article

Kleylein-Sohn et al Dev Cell. 2007 Aug;13(2):190-202.

Insert

Centrin-2 (CETN2 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-N-Flag-HA-IRES-PURO

Plasmid

#41033

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Type

Empty backbone

Use

Gateway Cloning and Retroviral

Tags

Flag, HA, and IRES Puro

Expression

Mammalian

Promoter

LTR-driven expression

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only

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