163,606 results

1. pAAV-CAG-ConVERGD-eGFP-W3SL

   Plasmid

   #218748

   Purpose

   Provides AND intersectional (Cre+Flp) expression of eGFP

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AA184

   Plasmid

   #216047

   Purpose

   Fragmid fragment: (C' terminus) for prime editing; using PEmax

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   SV40NLS_v1.2; MMLV-RT_v1.2; SV40NLS_v1.3; c-mycNLS_v1.1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AA063

   Plasmid

   #216020

   Purpose

   Fragmid fragment: (Cas protein) nickase Cas for prime editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   nCas9-SpRY (H840A)_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AA061

   Plasmid

   #216018

   Purpose

   Fragmid fragment: (Cas protein) nickase Cas for prime editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   nCas9-NG (H840A)_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AA058

   Plasmid

   #216040

   Purpose

   Fragmid fragment: (C' terminus) for prime editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   MMLV-RT_v1.1; SV40NLS_v1.3
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AA183

   Plasmid

   #216025

   Purpose

   Fragmid fragment: (Cas protein) for prime editing; R221K;N394K for increased efficiency

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   nCas9 (H840A)_v2.1 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AA062

   Plasmid

   #216019

   Purpose

   Fragmid fragment: (Cas protein) nickase Cas for prime editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   nCas9-SpG (H840A)_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AA060

   Plasmid

   #216017

   Purpose

   Fragmid fragment: (Cas protein) nickase Cas for prime editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   nCas9 (H840A)_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFlare9A-Plekha5E10 WT

   Plasmid

   #209576

   Purpose

   minigene construct for Plekha5 E10 alternative splicing

   Depositor

   Sika Zheng

   Article

   Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

   Insert

   Plekha5 (Plekha5 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRIPZ-NUDT18-C-FLAG-Puro

    Plasmid

    #195181

    Purpose

    Lentiviral vector for DOX-inducible NUDT18 gene expression with C-terminal FLAG tag. Gateway expression system. Selection by puromycin.

    Depositor

    Stephanie Roessler

    Insert

    NUDT18 (NUDT18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF1074

    Plasmid

    #143753

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFATC4 (NFATC4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBetaActin-TUBB2a-mScarlet

    Plasmid

    #191324

    Purpose

    Expresses fluorescent labeled beta Tubulin to measure microtubule density

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    TUBB2a-mScarlet (Tubb2a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter

    Plasmid

    #172597

    Purpose

    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.

    Depositor

    Daniel Kiss

    Article

    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

    Insert

    myc-PDGFRA-K627R (PDGFRA Human, Synthetic)
    

    Use

    Egfp-pest_reporter

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    PDGFRA-K627R

    Promoter

    CMV

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-sgTp53#3/Cre

    Plasmid

    #173622

    Purpose

    Expresses a Tp53-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Tp53 (Trp53 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-sgBrca2#2/Cre

    Plasmid

    #173628

    Purpose

    Expresses a Brca2-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Brca2 (Brca2 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-sgPten#1/Cre

    Plasmid

    #173645

    Purpose

    Expresses a Pten-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Pten (Pten Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLyGo-Bs-1

    Plasmid

    #163137

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACYCDuet1-tglB

    Plasmid

    #134676

    Purpose

    Expresses His6-TglBin E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Insert

    TglB
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-UbC-mNeonGreen

    Plasmid

    #124102

    Purpose

    Expression of the fluorescent protein mNeonGreen in mammalian cells

    Depositor

    Pantelis Tsoulfas

    Insert

    mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Codon usage was optimized for Homo Sapiens

    Promoter

    UbC

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSGNluc

    Plasmid

    #186705

    Purpose

    A high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real time

    Depositor

    Maria Leptin, Baubak Bajoghli

    Article

    Kuri et al J Cell Sci. 2017 Feb 1;130(3):648-657. d

    Inserts

    GFP
    luciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge