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170,465 results

Showing: 1961 - 1980 of 170465 results

  5. pET28-MBP-super TEV protease

    Plasmid
    #171782
    Purpose
    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    MBP-sTEV
    Tags
    Arg6 tag and Internal His6 tag
    Expression
    Bacterial
    Mutation
    No internal TEV cleavage site between the MBP and…
    Promoter
    T7
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171782-plasmid-map-sequence-id-443047

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  8. AAVS1_CAG_OsTIR1_F74G_S210A

    Plasmid
    #242189
    Purpose
    AID 2.0's higher degradation efficiency came with target-specific basal degradation and slower recovery rates. To address these limitations, we employed base-editing-mediated mutagenesis followed by s
    Depositor
    Mazhar Adli
    Article
    Xing et al Nat Commun. 2025 Jul 18;16(1):6631. doi:
    Insert
    CAG_OsTIR1_F74G_S210A
    Expression
    Mammalian
    Mutation
    S210A
    Promoter
    CAG
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    242189-plasmid-map-sequence-id-495018

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  12. pSynSRE-T-Luc

    Plasmid
    #60444
    Purpose
    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.
    Depositor
    Timothy Osborne
    Article
    Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.
    Insert
    HMG-CoA synthase promoter (-324/-225)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Promoter
    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)
    Available Since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    60444-plasmid-map-sequence-id-266122

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  14. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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  17. SMARCA4-NanoLuc Fusion Vector

    Plasmid
    #238638
    Purpose
    Express SMARCA4-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    SMARCA4 (SMARCA4 Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238638-plasmid-map-sequence-id-478498

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  18. ePE6d

    Plasmid
    #229029
    Purpose
    Expresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in rice
    Depositor
    Li Juan
    Article
    Xu et al Plant Biotechnol J. 2024 Sep 27. doi: 10
    Insert
    Cas9
    Use
    CRISPR
    Available Since
    Feb. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    229029-plasmid-map-sequence-id-462729

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  19. pLGB36

    Plasmid
    #135621
    Purpose
    Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection
    Depositor
    Laurie Comstock
    Article
    Ito et al mBio. 2020 Feb 4;11(1). pii: mBio.03238-
    Insert
    bfe3
    Use
    Bacteroides suicide vector with inducible counter…
    Tags
    signal sequence for periplasmic targeting
    Promoter
    Bacteroides promoter regualted by TetR transcript…
    Available Since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    135621-plasmid-map-sequence-id-267063

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Showing: 1961 - 1980 of 170465 results