Addgene

157,643 results

Showing: 1941 - 1960 of 157643 results

Results per page:

Sort results by:

pT7-7 asyn WT

Plasmid

#36046

Depositor

Hilal Lashuel

Article

Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

Insert

alpha-synuclein (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX_311-KRAB-dCas9

Plasmid

#96918

Purpose

for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

Depositor

William Hahn, David Root, John Doench

Article

Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

Insert

dCas9

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

dCas9

Promoter

EF1a

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PGK1p-Csy4-pA (Construct 2)

Plasmid

#55196

Purpose

Expresses Csy4 in Mammalian cells for site-specific RNA processing

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

Csy4/Cas6

Use

Synthetic Biology

Tags

6xHIS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Sfp pet29b C-terminal His Tag

Plasmid

#75015

Purpose

Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

Depositor

Michael Burkart

Article

Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

Insert

Sfp

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-MH6-Bsu

Plasmid

#163911

Purpose

Expresses Bsu large fragment for affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

Bsu polymerase

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mRFP-Rab7

Plasmid

#14436

Purpose

Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

Depositor

Ari Helenius

Article

Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

Insert

Rab 7 (RAB7A Canis familialis)

Use

Tags

mRFP

Expression

Mammalian

Mutation

3 silent mutations near the C-terminus

Promoter

Available since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA

Plasmid

#183776

Purpose

AAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

Depositor

Ben Deverman

Article

Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

Insert

Cre

Use

AAV

Tags

Expression

Mutation

NA

Promoter

CAG

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57-MCS1-P2A-MCS2 (Neo)

Plasmid

#89180

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-CMV-CD19 puro

Plasmid

#196634

Purpose

Express CD19

Depositor

Nicolas Manel

Article

Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

Insert

CD19 (CD19 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLAG_WT MDA5

Plasmid

#216798

Purpose

Expresses wild type (wt) melanoma differentiation-associated protein 5

Depositor

Sun Hur

Insert

melanoma differentiation-associated protein 5 (IFIH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcVpx.myc

Plasmid

#213388

Purpose

Generate lentiviral vector which increases gene delivery into primary human myeloid cells

Depositor

Nathaniel Landau

Article

Sunseri et al J Virol. 2011 Jul;85(13):6263-74. doi: 1

Insert

SIVmac vpx (vpx Simian Immunodeficiency Virus)

Use

Lentiviral

Tags

myc-6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-GFP

Plasmid

#11150

Purpose

Mammalian expression vector for expression of GFP (CAG promoter)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

EGFP from Aequorea victoria

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
human p53-(1-393)

Plasmid

#24859

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Full length.

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-FLAG-GFP-Tmem192

Plasmid

#134630

Purpose

lysosomal marker

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLViP-LifeAct-iRFP670

Plasmid

#229699

Purpose

Lentiviral expression of LifeAct-iRFP670 in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

Synthetic construct LifeAct-mTurquoise gene

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18-mini-Tn7T-Gm-lux

Plasmid

#64963

Purpose

mini-Tn7 luxCDABE transcriptional fusion vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

lux operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCMV_iPE-C_P2A_Csy4

Plasmid

#232428

Purpose

Expression plasmid for iPE-C prime editor RNPs with additional Csy4 protection

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

Insert

iPE-C–P2A–Csy4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS130

Plasmid

#26290

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Use

Drosophila transgenesis

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-CFP

Plasmid

#13030

Purpose

Mammalian expression vector for cloning your gene fused to CFP

Depositor

Doug Golenbock

Has Service

Cloning Grade DNA

Insert

cyan fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge