|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26745||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAddgene plasmid 13793
- Backbone size w/o insert (bp) 5200
Vector typeMammalian Expression, Cre/Lox ; FLP/FRT
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameiCre expression cassette
Speciesoptimized iCre expression cassette; optimized Flpo expression cassette
Insert Size (bp)2500
- Cloning method Restriction Enzyme
- 5′ cloning site PsiI (destroyed during cloning)
- 3′ cloning site PsiI (destroyed during cloning)
- 5′ sequencing primer mPGK-F, EF-1a Forward
- 3′ sequencing primer BGH-rev, SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The pDIRE plasmid is designed to express the optimized versions of the Cre (iCre) and Flp (Flpo) recombinases and is required for dual RMCE (dRMCE) as described in Osterwalder et al. "Dual RMCE for efficient re-engineering of mouse mutant alleles" Nature Methods (2010) DOI 10.1038/nmeth.1521
Protocol for using these plasmids can be found here: http://www.nature.com/protocolexchange/protocols/1906
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDIRE was a gift from Rolf Zeller (Addgene plasmid # 26745 ; http://n2t.net/addgene:26745 ; RRID:Addgene_26745)
For your References section:Dual RMCE for efficient re-engineering of mouse mutant alleles. Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J.. Nat Methods. 2010 Oct 17 10.1038/nmeth.1521 PubMed 20953177