161,674 results

1. pRGEN-CMV-CjCas9

   Plasmid

   #89752

   Purpose

   Expression of CjCas9 in mammalian cells

   Depositor

   Seokjoong Kim

   Article

   Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTrEno

   Plasmid

   #115474

   Purpose

   Eno-promoter driven expression vector for protein production in Trichoderma reesei

   Depositor

   Steve Decker

   Article

   Linger et al Biotechnol Biofuels. 2015 Mar 18;8:45. d

   Insert

   Cel7A
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Introns have been removed

   Promoter

   Trichoderma reesei Enolase (Eno) promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBPp65ADZpUw

   Plasmid

   #26234

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Aug 9. ():.

   Insert

   p65AD-Zip (RELA Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. paGFP-Omp25

   Plasmid

   #69598

   Purpose

   expresses GFP-labeled mitochondrial outer membrane protein 25

   Depositor

   David Sabatini

   Article

   Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

   Insert

   paGFP-Omp25 (Synj2bp Rat)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   photoactivatable GFP fused to rat Omp25 C-terminus

   Promoter

   CMV

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. ADRB2-Tango

   Plasmid

   #66220

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ADRB2 (ADRB2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-Tet

   Plasmid

   #138086

   Purpose

   Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles

   Depositor

   Eva Frickel

   Article

   Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV promoter with heptamerized tet‐operat…

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-Nluc

   Plasmid

   #180501

   Purpose

   Mammalian expression of Nluc

   Depositor

   Masaharu Somiya

   Article

   Somiya et al bioRxiv 2022.01.25.477620

   Insert

   Nluc
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-UbiC-GFP-ps

   Plasmid

   #183712

   Purpose

   GFP with palmitoylation signal

   Depositor

   Jeffrey Gerst

   Article

   Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

   Insert

   GFP with palmitoylation signal
   

   Use

   Lentiviral

   Tags

   palmitoylation signal

   Expression

   Mutation

   Promoter

   UbiC

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACYC-T7

   Plasmid

   #41187

   Depositor

   Dan Bolon

   Article

   Wayne et al J Biol Chem. 2007 Nov 30;282(48):35386-9

   Insert

   S. cerevisiae Hsp82
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid

    #217808

    Purpose

    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-PKI-GFP

    Plasmid

    #118482

    Purpose

    GFP-tagged Protein Kinase A inhibitor peptide.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    PKI-GFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABE puro K-Ras V12

    Plasmid

    #9052

    Purpose

    Retroviral expression of a constitutively active form of K-Ras

    Depositor

    William Hahn

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V. Constitutively active.

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. Halo-MTS

    Plasmid

    #124315

    Purpose

    Expresses mitochondria specific HaloTag protein

    Depositor

    Jin Wang

    Article

    Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

    Insert

    Halo tag
    

    Use

    Tags

    Mito specific seq

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Syn-CaM-V5-sTb(N)

    Plasmid

    #219783

    Purpose

    sTb(N) half of CaST for neuron expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CaM-V5-sTb(N)
    

    Use

    AAV

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. HTR1A-Tango

    Plasmid

    #66404

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    HTR1A (HTR1A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Beta1-GFP in pHcgreen

    Plasmid

    #69804

    Purpose

    Expresses Beta1-GFP integrin for normal cell transfection

    Depositor

    Martin Humphries

    Article

    Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71.

    Insert

    Beta1A integrin (ITGB1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-Con/Fon-mCherry

    Plasmid

    #137132

    Purpose

    Intersectional viral expression of mCherry in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR_5xUAS-BFP-WPRE_PGK-HygR-bGHpolyA

    Plasmid

    #216665

    Purpose

    Expresses Gal4 inducible BFP, with hygromycin resistance

    Depositor

    Leonardo Morsut

    Inserts

    BFP
    HygromycinR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. gCH27 (crCD55-4)

    Plasmid

    #217343

    Purpose

    crRNA targeting CD55

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    crCD55-4 (CD55 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.hSyn.iGluSnFR3.v857.IgK-NGR

    Plasmid

    #196227

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn iGluSnFR3 v857.NGR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and NGR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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