165,904 results

1. pRK5_mCherry_P2AT2A_GFP-nb-KS-FtoA

   Plasmid

   #238234

   Purpose

   For overexpression of mCherry_P2AT2A_GFP-nb-KS-FtoA

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   mCherry_P2AT2A_GFP-nb-KS-FtoA
   

   Expression

   Mammalian

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDB4581

   Plasmid

   #171124

   Purpose

   A kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

   Insert

   3_sAID
   

   Use

   Other

   Mutation

   non applicable

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-GFP11-Clathrin light chain

   Plasmid

   #70217

   Purpose

   Expresses GFP11-clathrin light chain in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP11-Clathrin light chain (CLTA Human)
   

   Tags

   GFP11

   Expression

   Mammalian

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pICH-roGFP2-ORP1

   Plasmid

   #191677

   Purpose

   Ti plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   redox(ro)GFP2-ORP1
   

   Use

   Binary agrobacterium ti plasmid

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti-iCas9-neo

   Plasmid

   #85400

   Purpose

   Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence

   Depositor

   Qin Yan

   Article

   Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

   Insert

   Flag-iCas9-P2A-GFP
   

   Use

   Lentiviral

   Tags

   Flag-iCas9-P2A-GFP

   Available Since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDH-hMALAT1

   Plasmid

   #118580

   Purpose

   to overexpress human MALAT1 in mammalian cells

   Depositor

   Jianjun Zhao

   Article

   Hu et al Leukemia. 2018 Mar 22. pii: 10.1038/s413

   Insert

   MALAT1 (MALAT1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTet-O-Ngn2-puro

   Plasmid

   #52047

   Purpose

   3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

   Depositor

   Marius Wernig

   Article

   Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

   Insert

   Ngn2 (Neurog2 Mouse)
   

   Use

   Lentiviral

   Tags

   T2A-and puromycin resistant cassette

   Expression

   Mammalian

   Promoter

   tet-on

   Available Since

   April 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. v5 eVLP gag-pro-pol

   Plasmid

   #228466

   Purpose

   Expresses MMLVgag-pro-pol(Q226P) for producing v5 eVLPs

   Depositor

   David Liu

   Article

   Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

   Insert

   MMLVgag-pro-pol(Q226P)
   

   Expression

   Mammalian

   Mutation

   Q226P

   Promoter

   CMV

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC19 Giles integrase

   Plasmid

   #170049

   Purpose

   pUC19 with Giles integrase

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Insert

   GIles Integrase
   

   Expression

   Bacterial

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2-MT GLI2 delta N

    Plasmid

    #17649

    Depositor

    Erich Roessler

    Article

    Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

    Insert

    GLI2 (GLI2 Human)
    

    Tags

    6x Myc

    Expression

    Mammalian

    Mutation

    Missing 328 N-terminal amino acids

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMK243 (Tet-OsTIR1-PURO)

    Plasmid

    #72835

    Purpose

    Expresses OsTIR1 under the control of the Tet promoter

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    AAVS1-Tet-OsTIR1-PURO-AAVS1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3 FLAG FBXW7alpha

    Plasmid

    #236470

    Purpose

    transient overexpression of FBXW7alpha in mammalian cells

    Depositor

    Michele Pagano

    Article

    Busino et al Nat Cell Biol. 2012 Mar 4;14(4):375-85.

    Insert

    FBXW7alpha (FBXW7 Human)
    

    Expression

    Mammalian

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid

    #221004

    Purpose

    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    CasRx 30nt processed direct repeat with SD1 mutation
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    DR1 A>T

    Promoter

    U6

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pY010 (pcDNA3.1-hAsCpf1)

    Plasmid

    #69982

    Purpose

    Expresses humanized AsCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hAsCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HyPer7.2DAAO-CAV

    Plasmid

    #168303

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the caveolae

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-CAV
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHCMV-VSVgSS-pAG-TM

    Plasmid

    #207318

    Purpose

    Functional incorporation of protein AG (pAG) fusion protein into envelope of virions

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    protein AG
    

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GW1-PercevalHR

    Plasmid

    #49082

    Purpose

    Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

    Depositor

    Gary Yellen

    Article

    Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

    Insert

    PercevalHR
    

    Expression

    Mammalian

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. tau/pET29b

    Plasmid

    #16316

    Depositor

    Peter Klein

    Article

    Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

    Insert

    tau (MAPT Human)
    

    Expression

    Bacterial

    Available Since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDAC439

    Plasmid

    #195237

    Purpose

    Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
    

    Expression

    Mammalian

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmScarlet-I3_C1

    Plasmid

    #189756

    Purpose

    C1 Cloning vector for mammalian cells incorporating mScarlet-I3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge