phOct4-EGFP
(Plasmid #38776)

• Depositing Lab: Wei Cui
• Publication: Gerrard et al Stem Cells. 2005;23(1):124-33.

Backbone

• Vector backbone: pEGFP-1
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Oct-4 promoter
• Species: H. sapiens (human)
• Insert Size (bp): 3950
• Entrez Gene: POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
• Promoter: human OCT4
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: TT CCC ATG TCA AGT AAG TGG GGT GG
• 3′ sequencing primer: CGA GAA GGC AAA ATC TGA AGC CAG G

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The human OCT4 promoter (hOCT4pr, from 67539 to 71490 in human DNA sequence with accession number AP000509) was amplified by PCR with primers: hOCT4pr-F (5'−TTCCCATGTCAAGTAAGTGGGGTGG-3') and hOCT4pr-R (5'-CGAGAAGGCAAAATCTGAAGCCAGG-3') using human genomic DNA (Promega G3041) as a template. The fragment was cloned into a TOPO vector and the hOCT4 promoter was subsequently cloned into vector pEGFP1 by insertion into HindIII and AgeI restriction enzyme sites upstream of EGFP.