|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47684||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerdescribed in Kato, et al. 1997 PMID 9374409
Vector typeNotch reporter construct
Growth in Bacteria
Gene/Insert nameTP-1 promoter
Insert Size (bp)300
Mutationhexamerized 50-bp EBNA2 response element of the TP-1 promoter (ERE-TP1)
- Promoter NA
/ Fusion Protein
- d1EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer NA
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Article Citing this Plasmid
This plasmid was constructed from the pGa981-6 plasmid by replacing the luciferase gene in the original pGA981-6 plasmid with d1EGFP using XhoI/SmaI sites.
The construction of pGA981-6 was described as: "Six copies of this oligonucleotide were cloned in the BamHI site:
5' strand GATCCCGACT CGTGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA............
3' strand ............GGCTGA GCACCCTTTT ACCCGCCTTC CCGTGGCACC CTTTTATCAT CTAG"
Please note that Addgene's sequencing results indicate that the first and fourth ERE-TP1 repeats differ from the above oligo sequence at the twelfth nucleotide (G->A). These differences may have occurred during construction of the original pGA981-6 plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:12XCSL-d1EGFP was a gift from Urban Lendahl (Addgene plasmid # 47684 ; http://n2t.net/addgene:47684 ; RRID:Addgene_47684)
For your References section:Recording Notch signaling in real time. Hansson EM, Teixeira AI, Gustafsson MV, Dohda T, Chapman G, Meletis K, Muhr J, Lendahl U. Dev Neurosci. 2006;28(1-2):118-27. 10.1159/000090758 PubMed 16508309