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12XCSL-d1EGFP
(Plasmid #47684)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47684 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGA981-6
  • Backbone manufacturer
    described in Kato, et al. 1997 PMID 9374409
  • Vector type
    Notch reporter construct

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TP-1 promoter
  • Insert Size (bp)
    300
  • Mutation
    hexamerized 50-bp EBNA2 response element of the TP-1 promoter (ERE-TP1)
  • Promoter NA
  • Tag / Fusion Protein
    • d1EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid was constructed from the pGa981-6 plasmid by replacing the luciferase gene in the original pGA981-6 plasmid with d1EGFP using XhoI/SmaI sites.

The construction of pGA981-6 was described as: "Six copies of this oligonucleotide were cloned in the BamHI site:
5' strand GATCCCGACT CGTGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA............
3' strand ............GGCTGA GCACCCTTTT ACCCGCCTTC CCGTGGCACC CTTTTATCAT CTAG"

Please note that Addgene's sequencing results indicate that the first and fourth ERE-TP1 repeats differ from the above oligo sequence at the twelfth nucleotide (G->A). These differences may have occurred during construction of the original pGA981-6 plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    12XCSL-d1EGFP was a gift from Urban Lendahl (Addgene plasmid # 47684 ; http://n2t.net/addgene:47684 ; RRID:Addgene_47684)
  • For your References section:

    Recording Notch signaling in real time. Hansson EM, Teixeira AI, Gustafsson MV, Dohda T, Chapman G, Meletis K, Muhr J, Lendahl U. Dev Neurosci. 2006;28(1-2):118-27. 10.1159/000090758 PubMed 16508309