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pQCXIP-GFP1-10
(Plasmid #68715)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68715 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pQCXIP
  • Backbone manufacturer
    Clontech
  • Modifications to backbone
    GFP1-10 was amplified by PCR. The PCR product was ligated into BamHI/EcoRI site of pQCXIP-POU5 (created by insertion of POU5 into NotI/BamHI sites of pQCXIP). pQCXIP-POU5-GFP1-10 was digested with NotI/BamHI, filled in, and religated to remove POU5 resulting in pQCXIP-GFP1-10.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP1-10
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

GFP1-10 covers the first to the 10th beta strands of GFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIP-GFP1-10 was a gift from Yutaka Hata (Addgene plasmid # 68715 ; http://n2t.net/addgene:68715 ; RRID:Addgene_68715)
  • For your References section:

    A new cell-based assay to evaluate myogenesis in mouse myoblast C2C12 cells. Kodaka M, Yang Z, Nakagawa K, Maruyama J, Xu X, Sarkar A, Ichimura A, Nasu Y, Ozawa T, Iwasa H, Ishigami-Yuasa M, Ito S, Kagechika H, Hata Y. Exp Cell Res. 2015 Aug 15;336(2):171-81. doi: 10.1016/j.yexcr.2015.06.015. Epub 2015 Jun 24. 10.1016/j.yexcr.2015.06.015 PubMed 26116467