165,744 results

1. pAF0474 Ef1a-Si74_attB acceptor

   Plasmid

   #193475

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Si74_attB
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p16sheLL

   Plasmid

   #37320

   Depositor

   John Schiller

   Article

   Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

   Insert

   HPV16L1+L2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pgRNA-humanized

   Plasmid

   #44248

   Purpose

   Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   guide RNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

   Plasmid

   #139989

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. FTL

   Plasmid

   #100750

   Purpose

   Part of FerriTag expression system. Untagged Ferritin light chain in mammalian expression vector.

   Depositor

   Stephen Royle

   Article

   Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

   Insert

   FTL (FTL Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Nb-gp41-Halo (MoonTag-Nb-Halo)

   Plasmid

   #128603

   Purpose

   Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Insert

   Nanobody_gp41
   

   Use

   Lentiviral

   Tags

   GB1 and Halo

   Promoter

   SFFV

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PCDNA3-GFP1-9

   Plasmid

   #121684

   Purpose

   PPI reporter

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

   Insert

   GFP1-9
   

   Expression

   Mammalian

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

   Plasmid

   #219537

   Purpose

   a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al bioRxiv 2024.11.06.622258

   Inserts

   Cre recombinase (cre )
   NLS-tdMCP-GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLIB

   Plasmid

   #80610

   Purpose

   pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4 [luc2P/GAS-RE/Hygro] Vector

    Plasmid

    #236813

    Purpose

    Express GAS response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    GAS (GAST Human)
    

    Use

    Luciferase

    Mutation

    None

    Promoter

    minP

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pSicoR-eIF1a-GFP-RPL10a

    Plasmid

    #159890

    Purpose

    Expresses GFP-RPL10a for ribosomal immunoprecipitation

    Depositor

    James Ellis

    Article

    Rodrigues et al Cell Rep. 2020 Mar 24;30(12):4179-4196.e

    Insert

    RPL10a (RPL10A Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Promoter

    eIF1a

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTripZ-Tet-ON mCherry

    Plasmid

    #187262

    Purpose

    Doxycyclin inducible expression of mCherry

    Depositor

    Alpha Yap

    Article

    Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

    Insert

    mCherry-NLS
    

    Use

    Lentiviral

    Tags

    mCherry

    Promoter

    CMV

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-P4M-SidMx2

    Plasmid

    #51472

    Purpose

    Biosensor for PtdIns4P

    Depositor

    Tamas Balla

    Article

    Hammond et al J Cell Biol. 2014 Apr 7.

    Inserts

    P4M-SidM(546-647)
    P4M-SidM(546-647)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-Sec16A

    Plasmid

    #36155

    Purpose

    Expresses Sec16A as a GFP fusion

    Depositor

    David Stephens

    Article

    Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

    Insert

    Sec16A (SEC16A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pH2B-mNG2(11)

    Plasmid

    #206043

    Purpose

    Plasmid expressing a H2B-mNG2(11) fusion in mammalian cells for complementation with the mNG2(1-10) fragment.

    Depositor

    Alisa Piekny

    Article

    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

    Insert

    H2B gene fused to the mNG2(11) fragment
    

    Use

    Synthetic Biology

    Tags

    H2B-mNG2(11)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-TRE-dCas9-Zim3-MeCP2

    Plasmid

    #220143

    Purpose

    doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

    Depositor

    Frank Buchholz

    Article

    Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

    Insert

    dCas9-Zim3-MeCP2
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    Tre-3G

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. TurboID-His6_pET21a

    Plasmid

    #107177

    Purpose

    expresses His6-tagged TurboID in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Bacterial

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAGM4723

    Plasmid

    #48015

    Purpose

    Please see depositor comments section.

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCS2FA CO Tol2 TPase

    Plasmid

    #133032

    Purpose

    Expression vector for production of codon optimized Tol2 transposase

    Depositor

    Charles Nathan Hancock

    Article

    Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

    Insert

    Codon optimized Tol2 Transposase
    

    Use

    In vitro expression

    Mutation

    Codon Optimized

    Available Since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSGDlucV3.0

    Plasmid

    #119760

    Purpose

    dual luciferase with FMDV StopGo sequences flanking MCS

    Depositor

    John Atkins

    Article

    Loughran et al Mol Cell. 2022 Oct 6;82(19):3745-3749.e2

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV40 and T7

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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