161,641 results
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Plasmid#103274PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-17-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-17-5p target (MIR17 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSABAD14-172
Plasmid#61976PurposePhoRadAC6 inteinDepositorInsertPhoRadAC6 intein
UseTagsGB1-H6ExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-17-3p
Plasmid#103273PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-17-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-17-3p target (MIR17 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Dectin1A-C-10
Plasmid#57367PurposeLocalization: Membrane, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertDectin1A (CLEC7A Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSARSF53-165
Plasmid#61817PurposePhoRadAdC6 inteinDepositorInsertPhoRadAdC6 intein
UseTagsH6-Smt3ExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-Ubiquitin Fusion Vector
Plasmid#236943PurposeExpress HaloTag(R)-Ubiquitin Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsHaloTag (R)ExpressionMammalianMutationNonePromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CAG-FLEX-ArchT
Plasmid#209779PurposeCre recombinase-dependent expression of ArchT without a fluorophore tag.DepositorInserttdTomato removed
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFUW mCherry
Plasmid#239564PurposeCytosolic expression of mCherryDepositorInsertmCherry
UseLentiviralTagsExpressionMutationPromoterUbCAvailable sinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRhobin2-SEC61B-IRES-NLS-mTagBFP-2xNLS
Plasmid#239024PurposeCo-overexpression of Rhobin2-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling markerDepositorInsertRhobin2-SEC61B (SEC61B Synthetic, Human)
UseTagsTagBFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB_TetON_mCherry_P2AT2A_GFP-nb-2xKS_FtoG
Plasmid#238244PurposeFor integration of mCherry_P2AT2A_GFP-nb-2xKS_FtoGDepositorInsertmCherry_P2AT2A_GFP-nb-2xKS_FtoG
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-NF2-CFP
Plasmid#235687PurposeExpress CFP tagged NF2 gene in mammalian cells using the CMV promoterDepositorInsertNF2 (CA12 Human)
UseLentiviralTagsCFPExpressionMammalianMutationPromoterAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
Poliovirus 1 PV1 isolate 09_05_19 3C protease
Plasmid#228647PurposeBacterial expression of the Polio 3C proteaseDepositorInsertPolio virus 3C protease
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry
Plasmid#231007PurposeProtein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.DepositorInsertE4F1 aa220-242 (E4F1 Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKJ0464
Plasmid#228716PurposeBacterial expression of T7 RNAP with E643G mutation; for protein production and purificationDepositorArticleInsertSUmo_T7pol_E643G
UseTagsExpressionBacterialMutationE643GPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28.His.3C.Tau2N4R
Plasmid#226396PurposeExpresses his-tagged, 3C-cleavable WT 2N4R tau for recombinant protein production in bacteriaDepositorInsertTau2N4R (MAPT synthetic, Human)
UseTagsHis.3CExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP-LBR(1-245)-GFP
Plasmid#222425PurposeTo express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cellsDepositorInsertLBR(1-245aa) (LBR Human)
UseTagsFKBP and GFPExpressionMammalianMutationTruncation (Includes aa 1-245)PromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1161
Plasmid#217964PurposeFor RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system (ThermoFisher).DepositorInsertHistone H3.1 (H3C1 Human)
UseTagseGFP, APEXExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1177
Plasmid#217961PurposeDual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.DepositorInsertHistone H3 (HIST2H3PS2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAND1-T2A-dTom-PGK-Neo
Plasmid#209838PurposeTomato reporter plasmid targeting the human HAND1 geneDepositorInsertHAND1-T2A-dTomato-PGK-Neo-HAND1 (HAND1 Human)
UseHuman targetingTagsExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHelper_AraC/pBad-Tnpase (camR)
Plasmid#213909PurposeArabinose-inducible himar1C9 transposase expression - cam resistanceDepositorInsertAraC/pBad ATc-inducible himar1C9 transposase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only