pET29a-YS14
(Plasmid #66890)

• Purpose: Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)
• Depositing Lab: Jung-Hyun Min
• Publication: Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2. doi: 10.1016/j.ab.2012.05.006. Epub 2012 May 19.

Backbone

• Vector backbone: pET29a
• Backbone size w/o insert (bp): 5371
• Total vector size (bp): 6983
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: His6-Thrombin-Xenopus laevis histones H2A
• Species: X. laevis (frog)
• Insert Size (bp): 498
• Promoter: T7
• Tags / Fusion Proteins:
  • His6 tag
  • Thrombin
  • H2A

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: TAATACGACTCACTATAGGG
• 3′ sequencing primer: TCGGCCAAGAGCAAGTGAGAATTC

Gene/Insert 2

• Gene/Insert name: Xenopus laevis histones H2B
• Species: X. laevis (frog)
• Insert Size (bp): 372
• Promoter: T7
• Tag / Fusion Protein:
  • H2B

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GAATTCAATAATTTTGTTTAACTT
• 3′ sequencing primer: GTCGACTTACTTGGCGCTGGTGTA

Gene/Insert 3

• Gene/Insert name: Xenopus laevis histones H3
• Species: X. laevis (frog)
• Insert Size (bp): 411
• Promoter: T7
• Tag / Fusion Protein:
  • H3

Cloning Information for Gene/Insert 3

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: GTCGACAATAATTTTGTTTAACTT
• 3′ sequencing primer: GCGGCCGCCTAAGCCCTCTCGCCTCG

Gene/Insert 4

• Gene/Insert name: Xenopus laevis histones H4-Thrombin
• Species: X. laevis (frog)
• Insert Size (bp): 363
• Promoter: T7
• Tags / Fusion Proteins:
  • H4
  • Thrombin

Cloning Information for Gene/Insert 4

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GCGGCCGCAATAATTTTGTTTAACTT
• 3′ sequencing primer: CTCGAGGCTGCCGCGCGGCACCAGG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: B. Bartholomew and K. Luger
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry