161,641 results

1. pINDUCER21-RORA

   Plasmid

   #51303

   Purpose

   Expression of RORA after doxycycline administration

   Depositor

   George Daley

   Article

   Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

   Insert

   RAR-related orphan receptor A (RORA Human)
   

   Use

   Lentiviral

   Tags

   n/a

   Expression

   Mutation

   full length ORF

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCVhygro-F-G9a

   Plasmid

   #41721

   Purpose

   Retroviral expression of human G9a in mammalian cells

   Depositor

   Kai Ge

   Article

   Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

   Insert

   G9a (EHMT2 Human)
   

   Use

   Retroviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. ADRB1-Tango

   Plasmid

   #66219

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ADRB1 (ADRB1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe-puro largeTcDNA

   Plasmid

   #14088

   Depositor

   William Hahn

   Article

   Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

   Insert

   Large T antigen (SV40gp6 simian virus)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-CaMKII-Jaws-KGC-tdTomato-ER2

   Plasmid

   #153534

   Purpose

   AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CaMKII promoter.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-KGC-tdTomato-ER2
   

   Use

   AAV

   Tags

   ER2, KGC, and tdTomato

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   CaMKII

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF3448

   Plasmid

   #144924

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TP63 (TP63 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 MYC-Sumo3Q89P

   Plasmid

   #48963

   Purpose

   Expresses MYC-Sumo3Q90P mutant in mammalian cells

   Depositor

   Guy Salvesen

   Article

   Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

   Insert

   SUMO3 (SUMO3 Human)
   

   Use

   Tags

   MYC (EQKLISEED)

   Expression

   Mammalian

   Mutation

   Mutated Q89P

   Promoter

   T7

   Available since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pTT5-hSDC1

   Plasmid

   #52326

   Purpose

   expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

   Depositor

   Gordon Laurie

   Article

   Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

   Insert

   SDC1 (SDC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R117Q mutation (R95Q according to numbering in Fi…

   Promoter

   CMV

   Available since

   Dec. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-eYFP

   Plasmid

   #104055

   Purpose

   An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV2, and AAV5

   Insert

   EYFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. EZ-Tet-pLKO-Blast

    Plasmid

    #85973

    Purpose

    Tet/Dox inducible shRNA lentivirus with blasticidin selection

    Depositor

    Cindy Miranti

    Article

    Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1 / TetO

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRc/RSV-m CBP-HA

    Plasmid

    #16701

    Depositor

    Richard Goodman

    Article

    Chrivia et al Nature. 1993 Oct 28. 365(6449):855-9.

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. T-2A-EGFP-PGK-Puro

    Plasmid

    #83344

    Purpose

    Donor vector to knocin EGFP into human Brachyury(T) locus

    Depositor

    James Thomson

    Article

    Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

    Insert

    Human Brachyury knockin homology arms (TBXT Human)
    

    Use

    Tags

    2A-EGFP

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc

    Plasmid

    #113899

    Purpose

    Mammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    TetR-HA-NLS-P2A-BSD-Myc
    

    Use

    Lentiviral

    Tags

    HA-Myc

    Expression

    Mutation

    Promoter

    CMV-MIE

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. 6 x DBE-luc

    Plasmid

    #153160

    Purpose

    Luciferase reporter plasmid for FOXTF

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    minP

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR-NLS-actin

    Plasmid

    #118380

    Purpose

    Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Donor-plasmid

    Tags

    Expression

    Mutation

    NLS

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBad-mKalama1

    Plasmid

    #14892

    Purpose

    Bacterial expression of mKalama1 blue fluorescent protein

    Depositor

    Robert Campbell

    Article

    Ai et al Biochemistry. 2007 May 22;46(20):5904-10

    Insert

    mKalama1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    The fluorescent protein was inserted between XhoI…

    Promoter

    Available since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

17. FoxO1-ADA-GFP

    Plasmid

    #35640

    Depositor

    Domenico Accili

    Article

    Qiang et al J Biol Chem. 2010 Aug 27;285(35):27396-4

    Insert

    FoxO1-ADA-GFP (Foxo1 Mouse)
    

    Use

    Tags

    Flag, GFP, and Myc

    Expression

    Mammalian

    Mutation

    T24A, S253D and S316A mutations

    Promoter

    CMV

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9(BB)-2A-tomato SOX2-sgRNA

    Plasmid

    #196190

    Purpose

    Editing human SOX2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    SOX2 (SOX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBI-p53 R175H/EGFP

    Plasmid

    #16544

    Depositor

    Bert Vogelstein

    Article

    Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R175H

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-ALK

    Plasmid

    #116712

    Purpose

    Lentiviral expression of ALK

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ALK (ALK Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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