163,545 results

1. pOG44_FLP_recombinase (pEHA1338)

   Plasmid

   #209087

   Purpose

   FLP recombinase for FRT-mediated integration

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Insert

   FLP recombinase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CL20_mEGFP_NUP98_KDM5A

   Plasmid

   #205872

   Purpose

   Express mEGFP-tagged fusion protein, NUP98_KDM5A from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   NUP98_KDM5A (NUP98, KDM5A Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTCF158 Human beta-2 microglobulin

   Plasmid

   #180457

   Purpose

   E. coli expression of class I MHC light chain

   Depositor

   John Altman

   Insert

   Human beta-2 microglobulin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. GW1-Peredox-mCitrine

   Plasmid

   #32386

   Depositor

   Gary Yellen

   Article

   Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

   Insert

   Peredox-mCitrine
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine

   Plasmid

   #50464

   Purpose

   Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promote

   Depositor

   Bryan Roth

   Insert

   hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCAS9-VP64_GFP

   Plasmid

   #61422

   Purpose

   Expresses dCAS9-VP64 activator with 2A GFP

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Concentrated Lentiviral Prep

   Insert

   dCAS9(D10A,H840A)-VP64_2A_GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and H840A in Cas9

   Promoter

   EF1A

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pfmh-hCry1

   Plasmid

   #25843

   Depositor

   Aziz Sancar

   Insert

   human Cryptochrome 1 (CRY1 Human)
   

   Use

   Tags

   flag, his, and myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 9, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pEF1α-Cre-2A-mScarlet

   Plasmid

   #207745

   Purpose

   Expression of the Cre recombinase linked to a mScarlet fluorescent marker

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   Cre-PGK-mScarlet
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pGal4BD-CRY2

   Plasmid

   #28243

   Purpose

   Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   Gal4BD

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+) Drp1

    Plasmid

    #34706

    Depositor

    David Chan

    Insert

    Drp1 (Dnm1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138150

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR1_BsmBI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    DR1 A>T

    Promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3-EF1a-i53

    Plasmid

    #216243

    Purpose

    Express i53 in mammalian cells as a HDR enhancer

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    i53
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDUAL CD81 (GFP)

    Plasmid

    #86980

    Purpose

    Lentiviral expression construct encoding CD81 and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    CD81 (CD81 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV/hPGK

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_crGE

    Plasmid

    #172933

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

    Depositor

    Arnold Boersma

    Article

    Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

    Insert

    CrowdingSensorGE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. iRFP-H2A

    Plasmid

    #158691

    Purpose

    This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    iRFP670
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCE059-SiT-Cas12a-[Activ]

    Plasmid

    #128136

    Purpose

    Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

    Depositor

    Randall Platt

    Article

    Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

    Insert

    AsCas12a-[Activ]-Triplex
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-Sec23A

    Plasmid

    #66609

    Purpose

    GFP fusion of Sec23A

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec23A (SEC23A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-NUP98

    Plasmid

    #229593

    Purpose

    Expresses EGFP-NUP98 fusion in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cterminal HA WDR59 pRK5

    Plasmid

    #46328

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    WDR59 (WDR59 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBABE-puro SV40 LT

    Plasmid

    #13970

    Depositor

    Thomas Roberts

    Article

    Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

    Insert

    SV40 LT (SV40gp6 )
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge