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pCMV5-hPPT1

Plasmid

#85815

Purpose

Expression of hPPT in mammalian cells

Depositor

Sandra Hofmann

Article

Lu et al Mol Genet Metab. 2010 Apr;99(4):374-8. d

Insert

human PPT1 (PPT1 Human)

Expression

Mammalian

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-2-EWSR1

Plasmid

#46384

Depositor

Heinz Gehring

Article

Pahlich et al Proteins. 2005 Oct 1;61(1):164-75.

Insert

EWSR1 (EWSR1 Human)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-Flpe:GFP

Plasmid

#13788

Purpose

Mammalian expression of enhanced form of Flp recombinase fused to GFP

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Flpe-GFP fusion protein

Use

Flp/frt

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pGTR

Plasmid

#63143

Purpose

PCR template to construct polycistronic tRNA-gRNA

Depositor

Yinong Yang

Article

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

gRNA-tRNA fusion

Use

CRISPR and Synthetic Biology

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

Plasmid

#60904

Purpose

The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAf-CRISPR-yA

Plasmid

#191015

Purpose

CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.

Depositor

Perng-Kuang Chang

Article

Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

Insert

yA

Use

CRISPR

Promoter

Aspergillus flavus U6

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn CaBLAM

Plasmid

#244227

Purpose

Bioluminescent reporter for calcium signaling in neurons

Depositor

Ute Hochgeschwender

Article

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Inserts

mNeonGreen
CaBLAM

Use

AAV

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_PKA-mEGFP

Plasmid

#233353

Purpose

CMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-H3-23

Plasmid

#55058

Purpose

Localization: Nucleus/Histones, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

H3 (H3C2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-HA-CAD

Plasmid

#46238

Purpose

For expression of HA and FLAG-tagged murine CAD in mammalian cells

Depositor

Brendan Manning

Article

Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

Insert

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-jRCaMP1a

Plasmid

#61562

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRCaMP1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-STAT1

Plasmid

#11987

Depositor

Steven Johnson

Insert

Signal transducer and activator of transcription 1 (STAT1 Human)

Tags

GFP

Expression

Mammalian

Mutation

A188T, Q718R

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-FLAG-hSF3B1-K700E

Plasmid

#82577

Purpose

Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

Depositor

Manoj Pillai

Article

Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

Insert

human SF3B1-K700E (SF3B1 Human)

Tags

FLAG peptide

Expression

Mammalian

Mutation

K700E

Promoter

CMV

Available Since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pQC-mCherryFP-GFP

Plasmid

#129102

Purpose

mCherryFP positive control

Depositor

Fangliang Zhang

Article

Birnbaum et al Cell Chem Biol. 2018 Aug 16;25(8):1038-1

Insert

mCherryFP

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAsp-AMA-gpdA-ptr

Plasmid

#191016

Purpose

CRISPR vector for cloning sgDNAs to target genes of several Aspergillus species (especially section Flavi)

Depositor

Perng-Kuang Chang

Article

Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

Type

Empty backbone

Use

Unspecified; Aspergillus

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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TRPML1-mCherry

Plasmid

#135189

Purpose

Expresses TRPML1 with C-terminus mCherry tag in mammalian cells.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TRPML1 (Mcoln1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.tgoGAP1

Plasmid

#78737

Purpose

Expression of Ca2+ sensor GAP1 in the trans Golgi apparatus

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

tgoGAP1

Tags

N terminal 81 amino acids of human galactosyltran…

Expression

Mammalian

Mutation

D117A/D119A/D163A in aequorin moiety

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pMXS-IRES-BLAST flag-CAD T456A S1406A S1859A

Plasmid

#169826

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported S6K, PKA, and Erk1/2 phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

T456A S1406A S1859A; TCCC -> AGTC silent mutat…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE2

Plasmid

#90336

Purpose

ATF6 - Unfolded protein response element (UPRE) gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

ATF6

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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SepRS(2)/pSertRNA(B4)/EF-Sep

Plasmid

#173897

Purpose

The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

Depositor

Jason W Chin

Article

Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

Inserts

SepRS(2)
pSertRNA
EF-Sep

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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