161,641 results

1. pcDNA3.1(+)-PKI-GFP

   Plasmid

   #118482

   Purpose

   GFP-tagged Protein Kinase A inhibitor peptide.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   PKI-GFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+) CircRNA Mini Vector

   Plasmid

   #60648

   Purpose

   Expression plasmid for expressing circular RNAs of a desired sequence

   Depositor

   Jeremy Wilusz

   Article

   Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

   Insert

   ZKSCAN1 (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

   Plasmid

   #217808

   Purpose

   Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   T7

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE puro K-Ras V12

   Plasmid

   #9052

   Purpose

   Retroviral expression of a constitutively active form of K-Ras

   Depositor

   William Hahn

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   G12V. Constitutively active.

   Promoter

   Available since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a T7pCONS TIR-1 sfGFP

   Plasmid

   #154463

   Purpose

   pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBT372

   Plasmid

   #125613

   Purpose

   Expresses evoCDA1-BE4max-NG in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   evoCDA1-BE4max-NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F23S A123V I195F

   Promoter

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB_rtTA_BsmBI

   Plasmid

   #126028

   Purpose

   PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Type

   Empty backbone

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Halo-MTS

   Plasmid

   #124315

   Purpose

   Expresses mitochondria specific HaloTag protein

   Depositor

   Jin Wang

   Article

   Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

   Insert

   Halo tag
   

   Use

   Tags

   Mito specific seq

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF1106

   Plasmid

   #143762

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   EPAS1 (EPAS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_5xUAS-BFP-WPRE_PGK-HygR-bGHpolyA

    Plasmid

    #216665

    Purpose

    Expresses Gal4 inducible BFP, with hygromycin resistance

    Depositor

    Leonardo Morsut

    Inserts

    BFP
    HygromycinR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Syn-CaM-V5-sTb(N)

    Plasmid

    #219783

    Purpose

    sTb(N) half of CaST for neuron expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CaM-V5-sTb(N)
    

    Use

    AAV

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HTR1A-Tango

    Plasmid

    #66404

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    HTR1A (HTR1A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-Con/Fon-mCherry

    Plasmid

    #137132

    Purpose

    Intersectional viral expression of mCherry in cells expressing both Cre AND Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. gCH27 (crCD55-4)

    Plasmid

    #217343

    Purpose

    crRNA targeting CD55

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    crCD55-4 (CD55 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.hSyn.iGluSnFR3.v857.IgK-NGR

    Plasmid

    #196227

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn iGluSnFR3 v857.NGR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and NGR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Syn-CD4-sTb(C)-M13-GFP

    Plasmid

    #219784

    Purpose

    sTb(C) half of CaST for neuron expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CD4-sTb(C)-M13-GFP
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA4-Vps34-Flag

    Plasmid

    #24398

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Vps34 (PIK3C3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. APEX2-GBP

    Plasmid

    #67651

    Purpose

    Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-GBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-iCre

    Plasmid

    #89573

    Purpose

    Expresses iCre under pCAG

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    iCre
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLT3-D835Y-MSCV-IRES-GFP

    Plasmid

    #184780

    Purpose

    Expresses FLT3 D835Y activating tyrosine kinase domain mutant in mammalian cells

    Depositor

    Timothy Ley

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Activating tyrosine kinase domain mutation (D835Y)

    Promoter

    MSCV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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