PurposeExpression of Jmjd3 in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17440||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepCS2+ NLSMT
- Backbone size w/o insert (bp) 4400
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Alt namejumonji domain containing 3
SpeciesM. musculus (mouse)
Insert Size (bp)5000
Entrez GeneKdm6b (a.k.a. 1700064E03Rik, Jmjd3)
/ Fusion Proteins
- 6xMyc (N terminal on backbone)
- Flag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Full length mouse JMJD3 with a C-terminal FLAG was PCR-amplified from MGC clone BC075632 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2-Jmjd3-F was a gift from Kai Ge (Addgene plasmid # 17440 ; http://n2t.net/addgene:17440 ; RRID:Addgene_17440)
For your References section:Identification of JmjC domain-containing UTX and JMJD3 as histone H3 lysine 27 demethylases. Hong S, Cho YW, Yu LR, Yu H, Veenstra TD, Ge K. Proc Natl Acad Sci U S A. 2007 Nov 20. 104(47):18439-44. 10.1073/pnas.0707292104 PubMed 18003914