|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24167||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6600
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)5400
Entrez GeneKDM6B (a.k.a. JMJD3, NEDCFSA)
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer Bglob-intron-F (Common Sequencing Primers)
This plasmid contains EAW90127.1 with an E433K mutation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-HA-JMJD3 was a gift from Kristian Helin (Addgene plasmid # 24167 ; http://n2t.net/addgene:24167 ; RRID:Addgene_24167)
For your References section:UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development. Agger K, Cloos PA, Christensen J, Pasini D, Rose S, Rappsilber J, Issaeva I, Canaani E, Salcini AE, Helin K. Nature. 2007 Oct 11. 449(7163):731-4. 10.1038/nature06145 PubMed 17713478