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pCMV-HA-JMJD3
(Plasmid #24167)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24167 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    JMJD3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5400
  • Entrez Gene
    KDM6B (a.k.a. JMJD3, NEDCFSA)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains EAW90127.1 with an E433K mutation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-HA-JMJD3 was a gift from Kristian Helin (Addgene plasmid # 24167 ; http://n2t.net/addgene:24167 ; RRID:Addgene_24167)
  • For your References section:

    UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development. Agger K, Cloos PA, Christensen J, Pasini D, Rose S, Rappsilber J, Issaeva I, Canaani E, Salcini AE, Helin K. Nature. 2007 Oct 11. 449(7163):731-4. 10.1038/nature06145 PubMed 17713478