pCIG3 (pCMV-IRES-GFP version 3)
(Plasmid #78264)

• Purpose: (Empty Backbone) Lentiviral empty cloning vector containing IRES and GFP
• Depositing Lab: Felicia Goodrum
• Publication: Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi: 10.1128/JVI.02711-14. Epub 2014 Oct 8.
• Citations: 21 Articles

Backbone

• Vector backbone: Derived from pCIG1
• Backbone manufacturer: Goodrum lab, Addgene plasmid 78265
• Backbone size (bp): 7624
• Vector type: Mammalian Expression, Lentiviral
• Promoter: CMV, lentiviral LTR
• Tag / Fusion Protein:
  • IRES-EGFP (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pCDH-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector differs from pCIG1 in the following manners:
- Different IRES (derived from Clontech pIRES-GFP), which is easier to mutagenize, PCR, and sanger sequence.
- Expanded MCS, derived from linker oligo cloning.
- No longer contains an unannotated second XbaI site. XbaI site in MCS can be used for cloning.