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179,803 results

Showing: 1681 - 1700 of 179803 results

  3. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Plasmid
    #179105
    Purpose
    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Publication
    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    179105-plasmid-map-sequence-id-421140

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  9. pLenti-sgRNA

    Plasmid
    #71409
    Purpose
    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI
    Depositor
    Eric Lander, David Sabatini
    Type
    Empty backbone
    Use
    Lentiviral
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71409-plasmid-map-sequence-id-322475

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  11. GPD-roGFP

    Plasmid
    #49436
    Purpose
    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells
    Depositor
    Paul Schumacker
    Publication
    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi
    Insert
    GPD-roGFP2
    Use
    Adenoviral
    Tags
    Glycerol phosphate dehydrogenase, 5' to roGF…
    Expression
    Mammalian
    Mutation
    C48S, Q80R, S147C, and Q204C in Clontech's G…
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    49436-plasmid-map-sequence-id-251833

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  12. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

    Plasmid
    #47868
    Purpose
    This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer, James Thomson
    Publication
    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.
    Inserts
    NmCas9
    U6pr-tracrRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a and U6
    Available since
    Sept. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47868-plasmid-map-sequence-id-68833

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  13. pAAV-FLEX-tdTomato

    Plasmid
    #28306
    Purpose
    Cre-dependent AAV-mediated expression of tdTomato under the CAG promoter
    Depositor
    Edward Boyden
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.S, AAV PHP.eB, and AAV Retrograde
    Insert
    tdTomato
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available since
    Sept. 7, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    28306-plasmid-map-sequence-id-520301

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Showing: 1681 - 1700 of 179803 results